Strain Name:
C57BL/6J-MtgxR5528Btlr/Mmmh
Stock Number:
043086-MU
Citation ID:
RRID:MMRRC_043086-MU
Other Names:
R5528 (G1), C57BL/6J-MtgxR5528Btlr
Major Collection:

Gene Information

Faah
Name: fatty acid amide hydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14073
HGNC: HGNC:3553
Homologene: 68184
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: ARPP-21, 0710001E13Rik, R3hdm3, Tarpp, D9Bwg1012e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74100
Homologene: 32306
Il17rd
Name: interleukin 17 receptor D
Synonyms: 2810004A10Rik, Sef, Sef-S
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 171463
VEGA: 14
Homologene: 9717
Ddx1
Name: DEAD box helicase 1
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104721
VEGA: 12
HGNC: HGNC:2734
Homologene: 3627
Lta4h
Name: leukotriene A4 hydrolase
Synonyms: LTA4 hydrodase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16993
VEGA: 10
HGNC: HGNC:6710
Homologene: 6820
Eif2ak4
Name: eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms: GCN2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27103
Homologene: 40891
Mtf2
Name: metal response element binding transcription factor 2
Synonyms: Pcl2, M96, 9230112N11Rik, C76717
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17765
Homologene: 7207
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: GC-GAP, 3426406O18Rik, p200RhoGAP, Grit, PX-RICS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330914
Homologene: 8812
Myo9b
Name: myosin IXb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Tshr
Name: thyroid stimulating hormone receptor
Synonyms: pet, hyt, hypothroid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22095
Homologene: 315
Ap3s2
Name: adaptor-related protein complex 3, sigma 2 subunit
Synonyms: [s]3B, sigma 3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11778
HGNC: HGNC:571
Homologene: 100592
Gucy1a1
Name: guanylate cyclase 1, soluble, alpha 1
Synonyms: sGC-alpha1, 1200016O07Rik, Gucy1a3, alpha 1 sGC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 60596
HGNC: HGNC:4685
Homologene: 37360
Lama5
Name: laminin, alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16776
HGNC: HGNC:6485
Homologene: 4060
Lmo7
Name: LIM domain only 7
Synonyms: FBXO20, LOC380928
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380928
HGNC: HGNC:6646
Homologene: 83924
Wnt3a
Name: wingless-type MMTV integration site family, member 3A
Synonyms: Wnt-3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22416
Homologene: 22528
Ablim2
Name: actin-binding LIM protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231148
Homologene: 82366
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 319262
Homologene: 14127
Eif2a
Name: eukaryotic translation initiation factor 2A
Synonyms: D3Ertd194e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229317
HGNC: HGNC:3254
Homologene: 5969
Zkscan8
Name: zinc finger with KRAB and SCAN domains 8
Synonyms: Zfp192, 2510038J07Rik, LD5-1, D430019P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 93681
Homologene: 21268
Nsun3
Name: NOL1/NOP2/Sun domain family member 3
Synonyms: 6720484A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106338
Homologene: 65358
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: IDPm, Idh-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Clcn1
Name: chloride channel, voltage-sensitive 1
Synonyms: Clc1, NMF355, Clc-1, SMCC1, nmf355
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12723
HGNC: HGNC:2019
Homologene: 63
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: 6820443O06Rik, Nesp2g, nesprin-2, syne-2, Cpfl8, diminished cone electroretinogram, dice, D12Ertd777e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319565
Homologene: 56700
Celsr2
Name: cadherin, EGF LAG seven-pass G-type receptor 2
Synonyms: flamingo, Adgrc2, mfmi1, EGFL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 53883
HGNC: HGNC:3231
Homologene: 1078
Cpne8
Name: copine VIII
Synonyms: 1500031E20Rik, 1200003E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66871
Homologene: 12049
Ifit2
Name: interferon-induced protein with tetratricopeptide repeats 2
Synonyms: Ifi54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 15958
HGNC: HGNC:5409
Homologene: 1187
Spaca6
Name: sperm acrosome associated 6
Synonyms: Ncrna00085, B230206P06Rik, 4930546H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75202
Homologene: 53443
1110002E22Rik
Name: RIKEN cDNA 1110002E22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 102634333
Homologene: 131709
Atg9b
Name: autophagy related 9B
Synonyms: Apg9l2, eONE, LOC213948, Nos3as
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 213948
Homologene: 72638
Fam102a
Name: family with sequence similarity 102, member A
Synonyms: C230093N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 98952
Homologene: 51943
Trmt1l
Name: tRNA methyltransferase 1 like
Synonyms: 1190005F20Rik, Trm1-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98685
Homologene: 12801
Pik3r5
Name: phosphoinositide-3-kinase regulatory subunit 5
Synonyms: Foap2, p101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320207
Homologene: 8627
Cdh4
Name: cadherin 4
Synonyms: R-Cadh, R-cadherin, Rcad
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12561
HGNC: HGNC:1763
Homologene: 48044
Cstdc7
Name: cystatin domain containing 7
Synonyms: Gm5689
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 435561
VEGA: 18
C9orf72
Name: C9orf72, member of C9orf72-SMCR8 complex
Synonyms: 3110043O21Rik, Dennd9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73205
Homologene: 10137
Phgdh
Name: 3-phosphoglycerate dehydrogenase
Synonyms: 3-PGDH, SERA, A10, PGAD, 3PGDH, PGD, PGDH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 236539
HGNC: HGNC:8923
Homologene: 39318
Klf11
Name: Kruppel-like factor 11
Synonyms: Tieg2, D12Ertd427e, Tieg2b, Tieg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 194655
Homologene: 2668
Ccdc33
Name: coiled-coil domain containing 33
Synonyms: LOC382077, 4930535E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382077
Homologene: 19641
Kcnq3
Name: potassium voltage-gated channel, subfamily Q, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110862
HGNC: HGNC:6297
Homologene: 20949
Hook3
Name: hook microtubule tethering protein 3
Synonyms: 5830454D03Rik, E330005F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320191
Homologene: 13022
Nlrp4e
Name: NLR family, pyrin domain containing 4E
Synonyms: 4930406H16Rik, Nalp4e, Nalp-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 446099
Homologene: 75315
Pde6b
Name: phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Synonyms: Pdeb, rd1, rd, rd10, r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18587
HGNC: HGNC:8786
Homologene: 237
4930519G04Rik
Name: RIKEN cDNA 4930519G04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67593
Homologene: 87032
Mkrn3
Name: makorin, ring finger protein, 3
Synonyms: Zfp127, D7H15S9-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22652
HGNC: HGNC:7114
Homologene: 4143
Vmn2r23
Name: vomeronasal 2, receptor 23
Synonyms: EG435916
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435916
Homologene: 135826
Ms4a19
Name: membrane-spanning 4-domains, subfamily A, member 19
Synonyms: 1700025F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 69416
Homologene: 137380
Esp15
Name: exocrine gland secreted peptide 15
Synonyms: Gm9041
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 668200
VEGA: 17
Gm7358
Name: predicted gene 7358
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 664831
Homologene: 133217
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 151,454,995 bp
  • G to A, chromosome 2 at 32,566,327 bp
  • G to A, chromosome 2 at 118,427,938 bp
  • T to A, chromosome 2 at 179,893,995 bp
  • T to A, chromosome 2 at 180,194,563 bp
  • G to T, chromosome 3 at 58,548,512 bp
  • A to T, chromosome 3 at 82,109,073 bp
  • T to C, chromosome 3 at 98,328,339 bp
  • A to T, chromosome 3 at 108,413,294 bp
  • T to C, chromosome 3 at 138,066,499 bp
  • T to C, chromosome 4 at 35,213,556 bp
  • G to T, chromosome 4 at 116,005,065 bp
  • T to C, chromosome 5 at 24,386,028 bp
  • C to T, chromosome 5 at 35,856,166 bp
  • T to A, chromosome 5 at 108,094,157 bp
  • A to G, chromosome 5 at 108,423,558 bp
  • T to G, chromosome 5 at 114,874,354 bp
  • C to A, chromosome 6 at 42,300,341 bp
  • A to G, chromosome 6 at 123,713,002 bp
  • A to C, chromosome 7 at 23,336,891 bp
  • T to C, chromosome 7 at 62,418,987 bp
  • A to T, chromosome 7 at 79,880,486 bp
  • AGGGCCAGGG to AGG, chromosome 7 at 80,098,341 bp
  • G to A, chromosome 7 at 105,703,209 bp
  • T to C, chromosome 8 at 26,072,293 bp
  • A to G, chromosome 8 at 71,323,274 bp
  • T to A, chromosome 9 at 32,247,076 bp
  • T to C, chromosome 9 at 58,028,795 bp
  • C to T, chromosome 9 at 112,149,353 bp
  • T to C, chromosome 10 at 93,471,874 bp
  • T to C, chromosome 11 at 59,275,280 bp
  • T to C, chromosome 11 at 68,495,977 bp
  • A to G, chromosome 12 at 13,229,294 bp
  • A to T, chromosome 12 at 24,654,930 bp
  • G to T, chromosome 12 at 75,890,455 bp
  • A to G, chromosome 12 at 91,537,193 bp
  • A to G, chromosome 13 at 21,520,725 bp
  • T to C, chromosome 14 at 27,088,067 bp
  • A to G, chromosome 14 at 101,902,086 bp
  • T to A, chromosome 15 at 66,025,178 bp
  • C to T, chromosome 15 at 90,619,690 bp
  • A to G, chromosome 16 at 62,735,326 bp
  • C to A, chromosome 17 at 14,058,364 bp
  • A to C, chromosome 17 at 17,831,082 bp
  • A to T, chromosome 17 at 39,644,749 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • A to G, chromosome 18 at 42,173,662 bp
  • T to C, chromosome 19 at 11,141,635 bp
  • T to A, chromosome 19 at 34,573,537 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5528 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043086-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.