Strain Name:
C57BL/6J-MtgxR5532Btlr/Mmmh
Stock Number:
043090-MU
Citation ID:
RRID:MMRRC_043090-MU
Other Names:
R5532 (G1), C57BL/6J-MtgxR5532Btlr
Major Collection:

Gene Information

Xrcc1
Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Synonyms: Xrcc-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22594
Homologene: 31368
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 78757
VEGA: 15
Homologene: 34317
Gatad2a
Name: GATA zinc finger domain containing 2A
Synonyms: 1110066C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234366
Homologene: 9766
Syce2
Name: synaptonemal complex central element protein 2
Synonyms: 1700013H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 71846
Homologene: 12414
Cenpv
Name: centromere protein V
Synonyms: Prr6, 3110013H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 73139
Homologene: 42965
Adsl
Name: adenylosuccinate lyase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 11564
HGNC: HGNC:291
Homologene: 12
Map4
Name: microtubule-associated protein 4
Synonyms: Mtap4, MAP 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Ano6
Name: anoctamin 6
Synonyms: F730003B03Rik, 2900059G15Rik, Tmem16f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 105722
VEGA: 15
Homologene: 27888
Ufd1
Name: ubiquitin recognition factor in ER-associated degradation 1
Synonyms: Ufd1, Ufd1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 22230
Homologene: 39090
Rsf1
Name: remodeling and spacing factor 1
Synonyms: XAP8, p325, 4832420A03Rik, C030033M12Rik, Hbxap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233532
Homologene: 41142
Nfrkb
Name: nuclear factor related to kappa B binding protein
Synonyms: A530090G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235134
HGNC: HGNC:7802
Homologene: 4492
Strada
Name: STE20-related kinase adaptor alpha
Synonyms: 2610019A05Rik, 6030402H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 72149
Homologene: 12448
Ubtf
Name: upstream binding transcription factor, RNA polymerase I
Synonyms: UBF, UBF1, A930005G04Rik, Tcfubf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 21429
Homologene: 7970
Gja8
Name: gap junction protein, alpha 8
Synonyms: Lop10, connexin 50, Cx50, Cnx50, Aey5, alpha 8 connexin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 14616
HGNC: HGNC:4281
Homologene: 3857
Zfp277
Name: zinc finger protein 277
Synonyms: NIRF4, 2410017E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 246196
VEGA: 12
Homologene: 11087
Map2
Name: microtubule-associated protein 2
Synonyms: repro4, Mtap2, G1-397-34, MAP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17756
HGNC: HGNC:6839
Homologene: 1779
2410141K09Rik
Name: RIKEN cDNA 2410141K09 gene
Synonyms: Gt(pU21)35Imeg, Gt(Ayu21)35Imeg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 76803
Homologene: 136362
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Fat2
Name: FAT atypical cadherin 2
Synonyms: Fath2, mKIAA0811, LOC245827, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Galns
Name: galactosamine (N-acetyl)-6-sulfate sulfatase
Synonyms: N-acetylgalactosamine-6-sulfate sulfatase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 50917
HGNC: HGNC:4122
Homologene: 55468
Il12rb2
Name: interleukin 12 receptor, beta 2
Synonyms: IL-12RB2, Ifnm, A930027I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 16162
HGNC: HGNC:5972
Homologene: 1197
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mgr1, Mass1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 110789
Homologene: 19815
Ccdc146
Name: coiled-coil domain containing 146
Synonyms: 4930528G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 75172
Homologene: 67159
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: BC048546, Ovos2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232400
Homologene: 45969
Vmn2r74
Name: vomeronasal 2, receptor 74
Synonyms: EG546980
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 546980
Homologene: 115466
Nudt12
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 12
Synonyms: 0610016O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 67993
Homologene: 44188
Vmn2r98
Name: vomeronasal 2, receptor 98
Synonyms: EG224552
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224552
Homologene: 115024
Npc1l1
Name: NPC1 like intracellular cholesterol transporter 1
Synonyms: Niemann-Pick disease, type C1, 9130221N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237636
HGNC: HGNC:7898
Homologene: 56585
AW551984
Name: expressed sequence AW551984
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244810
Olfr308
Name: olfactory receptor 308
Synonyms: GA_x6K02T2NHDJ-9786435-9787361, MOR104-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258614
Homologene: 27225
Olfr1099
Name: olfactory receptor 1099
Synonyms: MOR206-3, GA_x6K02T2Q125-48446067-48445129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258764
Homologene: 107003
Spsb4
Name: splA/ryanodine receptor domain and SOCS box containing 4
Synonyms: Ssb4, D030068E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 211949
Homologene: 25315
Kcnq3
Name: potassium voltage-gated channel, subfamily Q, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 110862
HGNC: HGNC:6297
Homologene: 20949
Mtnr1b
Name: melatonin receptor 1B
Synonyms: Mt2, Mel1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244701
HGNC: HGNC:7464
Homologene: 4350
Olfr889
Name: olfactory receptor 889
Synonyms: MOR162-2, GA_x6K02T2PVTD-31795028-31795957
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258475
Homologene: 138311
Plekhg6
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 6
Synonyms: LOC213522
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 213522
Homologene: 32395
Mmp28
Name: matrix metallopeptidase 28 (epilysin)
Synonyms: epilysin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 118453
Homologene: 41559
Gm13119
Name: predicted gene 13119
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433779
Homologene: 83161
Gm4951
Name: predicted gene 4951
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 240327
Arhgap18
Name: Rho GTPase activating protein 18
Synonyms: 4833419J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 73910
Homologene: 14135
Pabpc4l
Name: poly(A) binding protein, cytoplasmic 4-like
Synonyms: C330050A14Rik, EG241989
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 241989
Homologene: 66336
Ifi209
Name: interferon activated gene 209
Synonyms: Pyhin-1, Pyhin1, Ifix
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 236312
HGNC: HGNC:5395
Homologene: 115929
Klk1b16
Name: kallikrein 1-related peptidase b16
Synonyms: mGk-16, Klk16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16615
HGNC: HGNC:6364
Homologene: 68141
Tex36
Name: testis expressed 36
Synonyms: 4930404H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 73808
Homologene: 67014
Teddm2
Name: transmembrane epididymal family member 2
Synonyms: e9-2, 5830403L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240817
Homologene: 65346
Olfr919
Name: olfactory receptor 919
Synonyms: MOR171-23, GA_x6K02T2PVTD-32400678-32399743
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258432
VEGA: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 66,414,620 bp
  • A to T, chromosome 1 at 153,850,384 bp
  • T to C, chromosome 1 at 173,638,976 bp
  • T to A, chromosome 2 at 86,958,580 bp
  • T to C, chromosome 3 at 46,446,609 bp
  • T to C, chromosome 3 at 96,920,332 bp
  • A to T, chromosome 4 at 144,363,491 bp
  • A to G, chromosome 5 at 21,305,331 bp
  • G to A, chromosome 6 at 67,292,262 bp
  • T to C, chromosome 6 at 125,372,551 bp
  • T to A, chromosome 6 at 128,553,330 bp
  • T to A, chromosome 7 at 24,567,928 bp
  • T to C, chromosome 7 at 44,141,526 bp
  • C to T, chromosome 7 at 85,951,989 bp
  • A to C, chromosome 7 at 86,321,671 bp
  • G to A, chromosome 7 at 97,680,695 bp
  • A to T, chromosome 7 at 133,601,983 bp
  • G to A, chromosome 8 at 69,916,420 bp
  • A to T, chromosome 8 at 84,883,502 bp
  • T to C, chromosome 8 at 122,585,026 bp
  • A to G, chromosome 9 at 15,862,914 bp
  • C to T, chromosome 9 at 31,397,779 bp
  • C to T, chromosome 9 at 38,116,627 bp
  • T to C, chromosome 9 at 38,697,647 bp
  • T to C, chromosome 9 at 39,597,185 bp
  • C to T, chromosome 9 at 96,995,574 bp
  • T to A, chromosome 9 at 110,034,678 bp
  • T to A, chromosome 10 at 26,846,108 bp
  • A to G, chromosome 11 at 6,224,245 bp
  • T to A, chromosome 11 at 55,262,337 bp
  • G to A, chromosome 11 at 62,527,572 bp
  • T to C, chromosome 11 at 83,442,858 bp
  • A to G, chromosome 11 at 102,308,959 bp
  • G to T, chromosome 11 at 106,171,017 bp
  • A to C, chromosome 12 at 40,335,309 bp
  • A to T, chromosome 13 at 66,431,681 bp
  • A to T, chromosome 13 at 81,543,433 bp
  • A to G, chromosome 15 at 6,789,565 bp
  • A to T, chromosome 15 at 65,997,773 bp
  • C to G, chromosome 15 at 80,963,909 bp
  • C to T, chromosome 15 at 95,962,241 bp
  • T to G, chromosome 16 at 18,817,930 bp
  • T to C, chromosome 16 at 32,753,614 bp
  • T to C, chromosome 17 at 19,067,383 bp
  • A to T, chromosome 17 at 59,003,550 bp
  • A to G, chromosome 18 at 60,246,070 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5532 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043090-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.