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Strain Name:
C57BL/6J-MtgxR5533Btlr/Mmmh
Stock Number:
043091-MU
Citation ID:
RRID:MMRRC_043091-MU
Other Names:
R5533 (G1), C57BL/6J-MtgxR5533Btlr
Major Collection:

Strain Information

Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19213
Homologene: 10304
Foxa3
Name: forkhead box A3
Synonyms: Tcf-3g, Hnf3g, Hnf-3g, Tcf3g
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15377
HGNC: HGNC:5023
Homologene: 3308
Sox10
Name: SRY (sex determining region Y)-box 10
Synonyms: Sox21, gt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20665
VEGA: 15
Homologene: 5055
Nub1
Name: negative regulator of ubiquitin-like proteins 1
Synonyms: BS4, NY-REN-18, 4931404D21Rik, 6330412F12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53312
Homologene: 41108
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,199,727 bp
  • T to A, chromosome 1 at 28,778,082 bp
  • T to C, chromosome 1 at 44,773,281 bp
  • A to G, chromosome 1 at 74,626,591 bp
  • T to A, chromosome 1 at 136,098,375 bp
  • A to G, chromosome 2 at 12,160,350 bp
  • G to T, chromosome 2 at 17,393,268 bp
  • G to A, chromosome 2 at 19,447,158 bp
  • A to G, chromosome 2 at 85,975,453 bp
  • A to G, chromosome 2 at 121,207,746 bp
  • AGATGGTGATGGTG to AGATGGTG, chromosome 2 at 121,925,697 bp
  • T to A, chromosome 3 at 59,602,575 bp
  • A to G, chromosome 4 at 82,359,767 bp
  • A to G, chromosome 4 at 128,615,863 bp
  • A to T, chromosome 4 at 147,582,983 bp
  • A to T, chromosome 5 at 24,702,381 bp
  • T to C, chromosome 5 at 45,733,877 bp
  • A to T, chromosome 5 at 127,691,031 bp
  • C to T, chromosome 5 at 137,735,464 bp
  • C to T, chromosome 6 at 15,197,120 bp
  • G to A, chromosome 6 at 84,186,471 bp
  • G to A, chromosome 6 at 125,312,794 bp
  • T to A, chromosome 6 at 132,711,430 bp
  • T to C, chromosome 7 at 4,549,505 bp
  • G to T, chromosome 7 at 19,015,015 bp
  • T to A, chromosome 7 at 46,614,776 bp
  • T to A, chromosome 7 at 101,505,980 bp
  • T to C, chromosome 7 at 108,466,262 bp
  • T to C, chromosome 7 at 142,377,333 bp
  • T to C, chromosome 8 at 117,068,116 bp
  • A to T, chromosome 9 at 69,450,650 bp
  • G to A, chromosome 9 at 108,857,956 bp
  • A to T, chromosome 9 at 109,486,065 bp
  • T to A, chromosome 10 at 4,357,405 bp
  • C to T, chromosome 10 at 25,283,982 bp
  • A to C, chromosome 10 at 109,883,678 bp
  • A to G, chromosome 10 at 127,083,042 bp
  • C to A, chromosome 11 at 16,263,125 bp
  • T to C, chromosome 11 at 20,304,417 bp
  • T to C, chromosome 11 at 55,532,382 bp
  • T to C, chromosome 11 at 62,343,011 bp
  • T to C, chromosome 11 at 62,780,970 bp
  • T to C, chromosome 11 at 68,991,470 bp
  • A to G, chromosome 11 at 78,365,966 bp
  • T to G, chromosome 11 at 79,445,789 bp
  • G to A, chromosome 12 at 35,123,026 bp
  • G to A, chromosome 13 at 73,685,829 bp
  • T to A, chromosome 14 at 53,738,717 bp
  • A to T, chromosome 14 at 70,693,967 bp
  • A to T, chromosome 14 at 75,658,834 bp
  • G to T, chromosome 14 at 103,282,645 bp
  • T to C, chromosome 15 at 76,294,557 bp
  • G to A, chromosome 15 at 79,156,302 bp
  • A to G, chromosome 16 at 88,874,108 bp
  • A to G, chromosome 17 at 7,772,776 bp
  • C to A, chromosome 17 at 24,397,633 bp
  • T to C, chromosome 17 at 28,602,759 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • T to C, chromosome 18 at 24,674,300 bp
  • T to A, chromosome 19 at 34,233,916 bp
  • A to C, chromosome 19 at 37,593,770 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5533 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043091-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.