Strain Name:
C57BL/6J-MtgxR5533Btlr/Mmmh
Stock Number:
043091-MU
Citation ID:
RRID:MMRRC_043091-MU
Other Names:
R5533 (G1), C57BL/6J-MtgxR5533Btlr
Major Collection:

Gene Information

Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: A230020K14Rik, 5730405M06Rik, Rxrip13, N-CoR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Itga8
Name: integrin alpha 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19213
Homologene: 10304
Foxa3
Name: forkhead box A3
Synonyms: Hnf-3g, Tcf3g, Tcf-3g, Hnf3g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 15377
HGNC: HGNC:5023
Homologene: 3308
Sox10
Name: SRY (sex determining region Y)-box 10
Synonyms: gt, Sox21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 20665
VEGA: 15
Homologene: 5055
Nub1
Name: negative regulator of ubiquitin-like proteins 1
Synonyms: BS4, NY-REN-18, 6330412F12Rik, 4931404D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 53312
Homologene: 41108
Nfib
Name: nuclear factor I/B
Synonyms: E030026I10Rik, 6720429L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 18028
HGNC: HGNC:7785
Homologene: 4087
Srpk1
Name: serine/arginine-rich protein specific kinase 1
Synonyms: SR protein-specific kinase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20815
Homologene: 110962
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 27223
Homologene: 4137
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Phr1, C130061D10Rik, Pam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 105689
Homologene: 9005
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Mhdadsk9, Dsk9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Exoc6
Name: exocyst complex component 6
Synonyms: Sec15l1, Sec15, msec15, 4833405E05Rik, hbd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107371
VEGA: 19
Homologene: 41305
Akap12
Name: A kinase (PRKA) anchor protein (gravin) 12
Synonyms: Srcs5, SSeCKS, Tsga12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 83397
VEGA: 10
HGNC: HGNC:370
Homologene: 3740
Arfgef1
Name: ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Synonyms: D130059B05Rik, BIG1, ARFGEP1, P200, D730028O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 211673
Homologene: 4687
Zfp985
Name: zinc finger protein 985
Synonyms: Gm13154
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433804
Homologene: 133076
Xpo7
Name: exportin 7
Synonyms: Ranbp16, 4930506C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 65246
VEGA: 14
Homologene: 22857
Pfas
Name: phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Synonyms: Sofa, 4432409B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237823
HGNC: HGNC:8863
Homologene: 5970
Akap7
Name: A kinase (PRKA) anchor protein 7
Synonyms: Akap18, AKAP15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 432442
HGNC: HGNC:377
Homologene: 49463
Glt1d1
Name: glycosyltransferase 1 domain containing 1
Synonyms: 5730455A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 319804
Homologene: 16965
Casc4
Name: cancer susceptibility candidate 4
Synonyms: D130060C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 319996
Homologene: 16310
Lcorl
Name: ligand dependent nuclear receptor corepressor-like
Synonyms: Mlr1, A830039H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 209707
Homologene: 82325
Ltbr
Name: lymphotoxin B receptor
Synonyms: Ltar, LT beta-R, TNF-R-III, TNFRrp, TNFCR, TNFR2-RP, LTbetaR, Tnfbr, Tnfrsf3, LT-beta receptor, TNF receptor-related protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17000
HGNC: HGNC:6718
Homologene: 1753
Abca3
Name: ATP-binding cassette, sub-family A (ABC1), member 3
Synonyms: ABC-C, Abc3, 1810036E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 27410
HGNC: HGNC:33
Homologene: 37437
Foxp2
Name: forkhead box P2
Synonyms: D0Kist7, 2810043D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 114142
Homologene: 134404
Mocos
Name: molybdenum cofactor sulfurase
Synonyms: 1110018O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 68591
VEGA: 18
Homologene: 9931
Snx13
Name: sorting nexin 13
Synonyms: RGS-PX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217463
VEGA: 12
Homologene: 41011
Nav3
Name: neuron navigator 3
Synonyms: 9630020C08Rik, Pomfil1p, 4732483H20Rik, unc53H3, steerin 3, POMFIL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 260315
Homologene: 56688
Ptprh
Name: protein tyrosine phosphatase, receptor type, H
Synonyms: SAP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 545902
HGNC: HGNC:9672
Homologene: 37693
Slc26a6
Name: solute carrier family 26, member 6
Synonyms: Pat1, CFEX, B930010B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 171429
Homologene: 99903
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: sj, DHPR alpha1s, muscle dysgenesis, fmd, Cchl1a3, mdg, Cav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
Nebl
Name: nebulette
Synonyms: A630080F05Rik, 1200007O21Rik, Lnebl, D830029A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 74103
Homologene: 128431
Slc1a4
Name: solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
Synonyms: ASCT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 55963
Homologene: 20655
Agap2
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Synonyms: Centg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216439
VEGA: 10
Homologene: 86815
Slc6a19
Name: solute carrier family 6 (neurotransmitter transporter), member 19
Synonyms: B0AT1, 4632401C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 74338
Homologene: 52819
Pkd1l2
Name: polycystic kidney disease 1 like 2
Synonyms: 1700126L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 76645
Homologene: 124481
Vstm2a
Name: V-set and transmembrane domain containing 2A
Synonyms: Vstm2, 9330184N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 211739
Homologene: 17123
Foxn1
Name: forkhead box N1
Synonyms: whn, Hfh11, D11Bhm185e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 15218
Homologene: 2664
Olfr498
Name: olfactory receptor 498
Synonyms: GA_x6K02T2PBJ9-10795522-10796514, MOR204-36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258304
HGNC: null
Homologene: 133600
Zfp286
Name: zinc finger protein 286
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192651
Homologene: 56892
Fkbpl
Name: FK506 binding protein-like
Synonyms: WAF-1/CIP1 stabilizing protein 39, DIR1, Ppiase-X, NG7, WISp39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 56299
Homologene: 10529
Gm597
Name: predicted gene 597
Synonyms: LOC210962
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 210962
HGNC: null
Homologene: null
Tas2r122
Name: taste receptor, type 2, member 122
Synonyms: mGR22, T2R22, Tas2r22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 630845
HGNC: null
Homologene: 105455
Fndc1
Name: fibronectin type III domain containing 1
Synonyms: 1110027O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 68655
Homologene: null
Stk36
Name: serine/threonine kinase 36
Synonyms: Fused, 1700112N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269209
Homologene: 49432
Fbxw19
Name: F-box and WD-40 domain protein 19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235612
Homologene: 110776
Sergef
Name: secretion regulating guanine nucleotide exchange factor
Synonyms: DelGEF, Gnefr, Gef
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 27414
Homologene: 40812
Erich6b
Name: glutamate rich 6B
Synonyms: Gm46471, 4930564B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 108168181
VEGA: 14
Homologene: 67175
Nyap1
Name: neuronal tyrosine-phosphorylated phosphoinositide 3-kinase adaptor 1
Synonyms: Nyap1, 6430598A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 243300
Homologene: 18320
Tlr12
Name: toll-like receptor 12
Synonyms: LOC384059, Tlr11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 384059
Homologene: 135964
Pde2a
Name: phosphodiesterase 2A, cGMP-stimulated
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 207728
HGNC: HGNC:8777
Homologene: 1952
Glra1
Name: glycine receptor, alpha 1 subunit
Synonyms: oscillator, nmf11, ot, B230397M16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14654
HGNC: HGNC:4326
Homologene: 20083
Olfr1029
Name: olfactory receptor 1029
Synonyms: GA_x6K02T2Q125-47454152-47455126, MOR198-1P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258154
Homologene: 73972
Stambpl1
Name: STAM binding protein like 1
Synonyms: 1700095N21Rik, 8230401J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 76630
Homologene: 23253
Gulp1
Name: GULP, engulfment adaptor PTB domain containing 1
Synonyms: 3110030A04Rik, GULP, Ced6, 5730529O06Rik, CED-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70676
Homologene: 9467
Smpd5
Name: sphingomyelin phosphodiesterase 5
Synonyms: Gm10345
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 100503915
Homologene: 132205
Gm5709
Name: predicted gene 5709
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 435732
HGNC: null
Homologene: 33954
Krtap19-2
Name: keratin associated protein 19-2
Synonyms: Krtap16-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 170651
VEGA: 16
HGNC: null
Homologene: null
Ctsd
Name: cathepsin D
Synonyms: CD, CatD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 13033
HGNC: HGNC:2529
Homologene: 55616
Gm4978
Name: predicted gene 4978
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244913
VEGA: 9
HGNC: null
Homologene: null
Trav8-2
Name: T-cell receptor alpha variable 8-2
Synonyms: Gm14031
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 193587
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,199,727 bp
  • T to A, chromosome 1 at 28,778,082 bp
  • T to C, chromosome 1 at 44,773,281 bp
  • A to G, chromosome 1 at 74,626,591 bp
  • T to A, chromosome 1 at 136,098,375 bp
  • A to G, chromosome 2 at 12,160,350 bp
  • G to T, chromosome 2 at 17,393,268 bp
  • G to A, chromosome 2 at 19,447,158 bp
  • A to G, chromosome 2 at 85,975,453 bp
  • A to G, chromosome 2 at 121,207,746 bp
  • AGATGGTGATGGTG to AGATGGTG, chromosome 2 at 121,925,697 bp
  • T to A, chromosome 3 at 59,602,575 bp
  • A to G, chromosome 4 at 82,359,767 bp
  • A to G, chromosome 4 at 128,615,863 bp
  • A to T, chromosome 4 at 147,582,983 bp
  • A to T, chromosome 5 at 24,702,381 bp
  • T to C, chromosome 5 at 45,733,877 bp
  • A to T, chromosome 5 at 127,691,031 bp
  • C to T, chromosome 5 at 137,735,464 bp
  • C to T, chromosome 6 at 15,197,120 bp
  • G to A, chromosome 6 at 84,186,471 bp
  • G to A, chromosome 6 at 125,312,794 bp
  • T to A, chromosome 6 at 132,711,430 bp
  • T to C, chromosome 7 at 4,549,505 bp
  • G to T, chromosome 7 at 19,015,015 bp
  • T to A, chromosome 7 at 46,614,776 bp
  • T to A, chromosome 7 at 101,505,980 bp
  • T to C, chromosome 7 at 108,466,262 bp
  • T to C, chromosome 7 at 142,377,333 bp
  • T to C, chromosome 8 at 117,068,116 bp
  • A to T, chromosome 9 at 69,450,650 bp
  • G to A, chromosome 9 at 108,857,956 bp
  • A to T, chromosome 9 at 109,486,065 bp
  • T to A, chromosome 10 at 4,357,405 bp
  • C to T, chromosome 10 at 25,283,982 bp
  • A to C, chromosome 10 at 109,883,678 bp
  • A to G, chromosome 10 at 127,083,042 bp
  • C to A, chromosome 11 at 16,263,125 bp
  • T to C, chromosome 11 at 20,304,417 bp
  • T to C, chromosome 11 at 55,532,382 bp
  • T to C, chromosome 11 at 62,343,011 bp
  • T to C, chromosome 11 at 62,780,970 bp
  • T to C, chromosome 11 at 68,991,470 bp
  • A to G, chromosome 11 at 78,365,966 bp
  • T to G, chromosome 11 at 79,445,789 bp
  • G to A, chromosome 12 at 35,123,026 bp
  • G to A, chromosome 13 at 73,685,829 bp
  • T to A, chromosome 14 at 53,738,717 bp
  • A to T, chromosome 14 at 70,693,967 bp
  • A to T, chromosome 14 at 75,658,834 bp
  • G to T, chromosome 14 at 103,282,645 bp
  • T to C, chromosome 15 at 76,294,557 bp
  • G to A, chromosome 15 at 79,156,302 bp
  • A to G, chromosome 16 at 88,874,108 bp
  • A to G, chromosome 17 at 7,772,776 bp
  • C to A, chromosome 17 at 24,397,633 bp
  • T to C, chromosome 17 at 28,602,759 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • T to C, chromosome 18 at 24,674,300 bp
  • T to A, chromosome 19 at 34,233,916 bp
  • A to C, chromosome 19 at 37,593,770 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5533 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043091-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.