Strain Name:
C57BL/6J-MtgxR5544Btlr/Mmmh
Stock Number:
043102-MU
Citation ID:
RRID:MMRRC_043102-MU
Other Names:
R5544 (G1), C57BL/6J-MtgxR5544Btlr
Major Collection:

Gene Information

Rps6ka1
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: Rsk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20111
Homologene: 55703
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: Bruce, D630005A10Rik, A430040A19Rik, apollon, A430032G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12211
Homologene: 7248
Brd2
Name: bromodomain containing 2
Synonyms: D17H6S113E, Fsrg1, Ring3, Frg-1, Rnf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14312
HGNC: HGNC:1103
Homologene: 74540
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Ap4m1
Name: adaptor-related protein complex AP-4, mu 1
Synonyms: 4930443L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11781
HGNC: HGNC:574
Homologene: 3467
Dock7
Name: dedicator of cytokinesis 7
Synonyms: LOC242555, m, 3110056M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67299
Homologene: 23566
Bcan
Name: brevican
Synonyms: Cspg7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12032
Homologene: 7244
Sidt2
Name: SID1 transmembrane family, member 2
Synonyms: CGI-40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214597
Homologene: 22943
Mal
Name: myelin and lymphocyte protein, T cell differentiation protein
Synonyms: VIP17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17153
HGNC: HGNC:6817
Homologene: 7827
Neto2
Name: neuropilin (NRP) and tolloid (TLL)-like 2
Synonyms: 5530601C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74513
Homologene: 32387
Cdk2
Name: cyclin-dependent kinase 2
Synonyms: A630093N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12566
VEGA: 10
HGNC: HGNC:1771
Homologene: 74409
Arid3b
Name: AT rich interactive domain 3B (BRIGHT-like)
Synonyms: Bdp, Dri2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56380
Homologene: 4721
Ttn
Name: titin
Synonyms: D830007G01Rik, L56, 1100001C23Rik, mdm, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, shru, connectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Nprl2
Name: NPR2 like, GATOR1 complex subunit
Synonyms: 2810446G01Rik, NPR2L, NPRL2, Tusc4, G21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56032
Homologene: 4771
Ptprh
Name: protein tyrosine phosphatase, receptor type, H
Synonyms: SAP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545902
HGNC: HGNC:9672
Homologene: 37693
Fbxl13
Name: F-box and leucine-rich repeat protein 13
Synonyms: 4921539K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320118
Homologene: 27057
Pcdhb13
Name: protocadherin beta 13
Synonyms: PcdhbM, Pcdbh6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93884
HGNC: HGNC:8691
Homologene: 10338
Vmn1r231
Name: vomeronasal 1 receptor 231
Synonyms: V1re7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171230
Hecw2
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Synonyms: A730039N16Rik, Nedl2, D030049F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329152
Homologene: 66192
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53419
Homologene: 4804
Sel1l3
Name: sel-1 suppressor of lin-12-like 3 (C. elegans)
Synonyms: 2310045A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231238
Homologene: 9054
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268527
Homologene: 8780
Thsd7a
Name: thrombospondin, type I, domain containing 7A
Synonyms: LOC330267
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330267
Homologene: 46582
Kdr
Name: kinase insert domain protein receptor
Synonyms: VEGF receptor-2, vascular endothelial growth factor receptor- 2, Flk1, Flk-1, VEGFR2, VEGFR-2, orv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16542
HGNC: HGNC:6307
Homologene: 55639
Spata20
Name: spermatogenesis associated 20
Synonyms: Tisp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217116
Homologene: 11264
Retreg2
Name: reticulophagy regulator family member 2
Synonyms: MGC47289, Fam134a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227298
Homologene: 11507
Cyp17a1
Name: cytochrome P450, family 17, subfamily a, polypeptide 1
Synonyms: steroid 17-alpha hydroxylase, Cyp17, p450c17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13074
HGNC: HGNC:2593
Homologene: 73875
C9
Name: complement component 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12279
HGNC: HGNC:1358
Homologene: 74406
Lamc2
Name: laminin, gamma 2
Synonyms: nicein, 100kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16782
HGNC: HGNC:6493
Homologene: 4062
Pcdhb17
Name: protocadherin beta 17
Synonyms: Pcdhb16, PcdhbQ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93888
Homologene: 81881
Slc16a11
Name: solute carrier family 16 (monocarboxylic acid transporters), member 11
Synonyms: MNCb-2717
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216867
Homologene: 27409
Adamts8
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8
Synonyms: METH-2, METH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 30806
HGNC: HGNC:224
Homologene: 5108
Map4k2
Name: mitogen-activated protein kinase kinase kinase kinase 2
Synonyms: Rab8ip, BL44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 26412
HGNC: HGNC:6864
Homologene: 3370
Rptn
Name: repetin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20129
Homologene: 84780
Wdr81
Name: WD repeat domain 81
Synonyms: shakey 5, nur5, MGC32441
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192652
Homologene: 13983
R3hdml
Name: R3H domain containing-like
Synonyms: OTTMUSG00000001070
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100043899
Homologene: 45608
Morn4
Name: MORN repeat containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226123
VEGA: 19
Homologene: 16635
Nlrp5-ps
Name: NLR family, pyrin domain containing 5, pseudogene
Synonyms: Gm18756
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100417675
Gm7347
Name: predicted gene 7347
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 664804
Homologene: 69402
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 53,942,659 bp
  • T to G, chromosome 1 at 75,144,689 bp
  • T to C, chromosome 1 at 153,124,053 bp
  • T to C, chromosome 2 at 76,726,538 bp
  • T to A, chromosome 2 at 127,635,017 bp
  • A to G, chromosome 2 at 163,498,422 bp
  • A to G, chromosome 3 at 87,993,053 bp
  • A to G, chromosome 3 at 93,398,473 bp
  • T to C, chromosome 4 at 98,967,257 bp
  • A to G, chromosome 4 at 133,872,015 bp
  • A to G, chromosome 5 at 21,524,491 bp
  • T to A, chromosome 5 at 26,055,018 bp
  • A to T, chromosome 5 at 53,200,302 bp
  • A to T, chromosome 5 at 72,305,014 bp
  • G to A, chromosome 5 at 75,960,743 bp
  • A to G, chromosome 5 at 138,178,370 bp
  • G to T, chromosome 6 at 12,379,471 bp
  • C to A, chromosome 7 at 4,580,910 bp
  • G to A, chromosome 7 at 14,580,975 bp
  • A to G, chromosome 8 at 85,647,877 bp
  • G to A, chromosome 9 at 30,952,703 bp
  • A to T, chromosome 9 at 45,944,455 bp
  • T to A, chromosome 9 at 57,798,097 bp
  • T to C, chromosome 9 at 107,544,609 bp
  • A to C, chromosome 10 at 128,699,139 bp
  • T to A, chromosome 11 at 70,215,000 bp
  • T to C, chromosome 11 at 75,441,797 bp
  • T to A, chromosome 11 at 94,482,875 bp
  • C to A, chromosome 12 at 16,673,796 bp
  • T to G, chromosome 12 at 40,834,702 bp
  • T to A, chromosome 15 at 6,497,027 bp
  • A to G, chromosome 17 at 20,890,578 bp
  • A to T, chromosome 17 at 34,113,334 bp
  • A to C, chromosome 17 at 74,670,374 bp
  • T to C, chromosome 18 at 37,443,520 bp
  • T to A, chromosome 18 at 37,487,421 bp
  • A to C, chromosome 19 at 6,345,914 bp
  • T to C, chromosome 19 at 42,076,247 bp
  • A to G, chromosome 19 at 46,672,654 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5544 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043102-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.