Strain Name:
C57BL/6J-MtgxR5545Btlr/Mmmh
Stock Number:
043103-MU
Citation ID:
RRID:MMRRC_043103-MU
Other Names:
R5545 (G1), C57BL/6J-MtgxR5545Btlr
Major Collection:

Gene Information

Ptprz1
Name: protein tyrosine phosphatase, receptor type Z, polypeptide 1
Synonyms: PTPzeta, PTPbeta, Rptpbeta, Ptpz, phosphacan, DSD-1-PG, RPTPz, Ptprz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Arhgef18
Name: rho/rac guanine nucleotide exchange factor (GEF) 18
Synonyms: A430078G23Rik, D030053O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319493
Homologene: 32254
Ube3a
Name: ubiquitin protein ligase E3A
Synonyms: Hpve6a, A130086L21Rik, 5830462N02Rik, E6-AP ubiquitin protein ligase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22215
Homologene: 7988
Tbr1
Name: T-box brain gene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21375
Homologene: 4807
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268902
Homologene: 43188
Prox1
Name: prospero homeobox 1
Synonyms: A230003G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19130
HGNC: HGNC:9459
Homologene: 2069
Ifnar2
Name: interferon (alpha and beta) receptor 2
Synonyms: Ifnar-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 15976
HGNC: HGNC:5433
Homologene: 49242
Ralbp1
Name: ralA binding protein 1
Synonyms: RLIP76, Rip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19765
VEGA: 17
HGNC: HGNC:9841
Homologene: 4940
Sorl1
Name: sortilin-related receptor, LDLR class A repeats-containing
Synonyms: mSorLA, LR11, 2900010L19Rik, Sorla
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20660
Homologene: 2336
Cyp20a1
Name: cytochrome P450, family 20, subfamily a, polypeptide 1
Synonyms: A930011N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77951
Homologene: 18584
Nbeal1
Name: neurobeachin like 1
Synonyms: A530083I02Rik, A530050O19Rik, 2310076G13Rik, ALS2CR17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269198
Homologene: 16453
Cdh6
Name: cadherin 6
Synonyms: K-cadherin, cad6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12563
VEGA: 15
HGNC: HGNC:1765
Homologene: 21027
Cngb1
Name: cyclic nucleotide gated channel beta 1
Synonyms: Cngb1b, BC016201, Cngb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 333329
HGNC: HGNC:2151
Homologene: 136420
Kcnd2
Name: potassium voltage-gated channel, Shal-related family, member 2
Synonyms: Kv4.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16508
HGNC: HGNC:6238
Homologene: 40828
Ttn
Name: titin
Synonyms: mdm, 2310036G12Rik, D830007G01Rik, shru, L56, 2310074I15Rik, 2310057K23Rik, connectin, D330041I19Rik, 1100001C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Ptpn13
Name: protein tyrosine phosphatase, non-receptor type 13
Synonyms: PTP-BL, PTPL1, Ptpri
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19249
HGNC: HGNC:9646
Homologene: 7909
Zc3h7a
Name: zinc finger CCCH type containing 7 A
Synonyms: Zc3h7, A430104C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106205
Homologene: 8563
Wdr90
Name: WD repeat domain 90
Synonyms: 3230401M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106618
Homologene: 27066
Nfatc2ip
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 interacting protein
Synonyms: NIP45, D7Ertd304e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18020
Homologene: 7862
Herc6
Name: hect domain and RLD 6
Synonyms: 2510038N07Rik, 4930427L17Rik, 1700121D12Rik, CEB1, Herc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67138
Homologene: 70768
2410004B18Rik
Name: RIKEN cDNA 2410004B18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66421
Homologene: 11968
Olfr267
Name: olfactory receptor 267
Synonyms: MOR262-1, GA_x6K02T2N78B-1272842-1273783
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 258922
HGNC: HGNC:8264
Homologene: 17436
Olfr1394
Name: olfactory receptor 1394
Synonyms: GA_x6K02T2QP88-6274566-6273628, MOR280-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258273
Homologene: 138305
Plin1
Name: perilipin 1
Synonyms: perilipin B, Peri, perilipin A, 6030432J05Rik, Plin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 103968
HGNC: HGNC:9076
Homologene: 2001
Akr1c19
Name: aldo-keto reductase family 1, member C19
Synonyms: 1810010N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432720
Homologene: 134145
Tmem229b
Name: transmembrane protein 229B
Synonyms: 6330442E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268567
Homologene: 18700
Acot5
Name: acyl-CoA thioesterase 5
Synonyms: PTE-Ic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217698
Homologene: 77351
Rsl1d1
Name: ribosomal L1 domain containing 1
Synonyms: 2410005K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66409
Homologene: 41052
Plekhg2
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 2
Synonyms: Clg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101497
Homologene: 16341
Scrn3
Name: secernin 3
Synonyms: 4833415E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74616
Homologene: 11601
Pate10
Name: prostate and testis expressed 10
Synonyms: Gm17677
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100312949
Homologene: 130079
Nlrp5-ps
Name: NLR family, pyrin domain containing 5, pseudogene
Synonyms: Gm18756
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100417675
Efhd1os
Name: EF hand domain containing 1, opposite strand
Synonyms: Gm16535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 60,283,983 bp
  • T to C, chromosome 1 at 60,376,082 bp
  • A to G, chromosome 1 at 87,264,614 bp
  • T to A, chromosome 1 at 190,147,142 bp
  • T to G, chromosome 2 at 61,807,376 bp
  • T to A, chromosome 2 at 73,335,781 bp
  • T to C, chromosome 2 at 76,764,376 bp
  • T to C, chromosome 3 at 145,939,098 bp
  • T to G, chromosome 4 at 58,785,585 bp
  • A to G, chromosome 5 at 103,561,964 bp
  • A to G, chromosome 6 at 21,217,019 bp
  • C to A, chromosome 6 at 23,050,545 bp
  • A to T, chromosome 6 at 57,658,007 bp
  • G to A, chromosome 7 at 14,580,975 bp
  • T to C, chromosome 7 at 28,362,461 bp
  • C to T, chromosome 7 at 59,272,024 bp
  • T to C, chromosome 7 at 79,726,509 bp
  • T to A, chromosome 7 at 126,390,470 bp
  • C to T, chromosome 8 at 3,453,127 bp
  • A to G, chromosome 8 at 95,252,173 bp
  • A to G, chromosome 9 at 35,741,644 bp
  • T to A, chromosome 9 at 41,991,625 bp
  • G to A, chromosome 11 at 49,160,626 bp
  • A to G, chromosome 12 at 78,964,809 bp
  • G to A, chromosome 12 at 84,069,606 bp
  • A to T, chromosome 13 at 4,242,595 bp
  • T to C, chromosome 15 at 13,041,149 bp
  • T to C, chromosome 16 at 11,148,451 bp
  • A to G, chromosome 16 at 11,199,650 bp
  • A to C, chromosome 16 at 73,961,747 bp
  • G to A, chromosome 16 at 91,385,025 bp
  • C to T, chromosome 17 at 25,845,856 bp
  • C to T, chromosome 17 at 65,850,104 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5545 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043103-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.