Strain Name:
C57BL/6J-MtgxR5549Btlr/Mmmh
Stock Number:
043106-MU
Citation ID:
RRID:MMRRC_043106-MU
Other Names:
R5549 (G1), C57BL/6J-MtgxR5549Btlr
Major Collection:

Gene Information

Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: D530039E11Rik, 6030405M08Rik, 4921505C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: LCMT-1, Lcmt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 30949
Homologene: 41123
Map3k8
Name: mitogen-activated protein kinase kinase kinase 8
Synonyms: c-COT, Cot, Tpl2, Cot/Tpl2, Tpl-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 26410
HGNC: HGNC:6860
Homologene: 3812
Diaph3
Name: diaphanous related formin 3
Synonyms: Drf3, 4930417P13Rik, Diap3, mDia2, p134MDia2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Ankrd11
Name: ankyrin repeat domain 11
Synonyms: Yod, 3010027A04Rik, 9530048I21Rik, 2410104C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 77087
Homologene: 69134
Sipa1l3
Name: signal-induced proliferation-associated 1 like 3
Synonyms: 2610511M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74206
Homologene: 77938
Nr2c1
Name: nuclear receptor subfamily 2, group C, member 1
Synonyms: TR2, Tr2-11, 4831444H07Rik, Eenr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22025
HGNC: HGNC:7971
Homologene: 55731
Ift122
Name: intraflagellar transport 122
Synonyms: C86139, sopb, Wdr10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 81896
Homologene: 12819
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Fabp3
Name: fatty acid binding protein 3, muscle and heart
Synonyms: Fabph4, Mdgi, Fabph1, Fabp3, H-FABP, Fabph-4, Fabph-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14077
HGNC: HGNC:3557
Homologene: 68379
Mobp
Name: myelin-associated oligodendrocytic basic protein
Synonyms: MOBP155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17433
HGNC: HGNC:7189
Homologene: 32040
Arhgap23
Name: Rho GTPase activating protein 23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 58996
Homologene: 104145
Sox2
Name: SRY (sex determining region Y)-box 2
Synonyms: lcc, ysb, Sox-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20674
Homologene: 68298
Zfp369
Name: zinc finger protein 369
Synonyms: NRIF2, B930030B22Rik, D230020H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 170936
Homologene: 129707
Fgf22
Name: fibroblast growth factor 22
Synonyms: 2210414E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67112
VEGA: 10
HGNC: HGNC:3679
Homologene: 10770
Nfkb2
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
Synonyms: NF kappaB2, p52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18034
VEGA: 19
HGNC: HGNC:7795
Homologene: 1873
Slc25a47
Name: solute carrier family 25, member 47
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104910
VEGA: 12
Homologene: 64441
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, Polydom, 1110021D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64817
Homologene: 23386
Zfp791
Name: zinc finger protein 791
Synonyms: EG244556
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244556
Homologene: 27420
Gm2962
Name: predicted pseudogene 2962
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100504052
Ccr5
Name: chemokine (C-C motif) receptor 5
Synonyms: CD195, Cmkbr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12774
HGNC: HGNC:1606
Homologene: 37325
C5ar2
Name: complement component 5a receptor 2
Synonyms: E030029A11Rik, C5L2, Gpr77
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 319430
HGNC: HGNC:4527
Homologene: 49549
Zfp941
Name: zinc finger protein 941
Synonyms: BC066028
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 407812
Oplah
Name: 5-oxoprolinase (ATP-hydrolysing)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75475
HGNC: HGNC:8149
Homologene: 90938
Zkscan3
Name: zinc finger with KRAB and SCAN domains 3
Synonyms: Skz1, Zfp307, Zfp306, 2810435N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72739
Homologene: 130732
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Mprip
Name: myosin phosphatase Rho interacting protein
Synonyms: Rhoip3, Gm34094, p116Rip, p116 Rho interacting protein, RIP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26936
Homologene: 9034
Grik3
Name: glutamate receptor, ionotropic, kainate 3
Synonyms: Glur7, Glur-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14807
HGNC: HGNC:4581
Homologene: 73901
Hecw2
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Synonyms: D030049F17Rik, Nedl2, A730039N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329152
Homologene: 66192
Mcemp1
Name: mast cell expressed membrane protein 1
Synonyms: 1810033B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69189
Homologene: 52162
Flnc
Name: filamin C, gamma
Synonyms: actin binding protein 280, 1110055E19Rik, Fln2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Atf6b
Name: activating transcription factor 6 beta
Synonyms: Crebl1, ATF6beta, Creb-rp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12915
HGNC: HGNC:2349
Homologene: 31238
4933427D14Rik
Name: RIKEN cDNA 4933427D14 gene
Synonyms: Gm43951
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74477
Homologene: 8874
Klhdc7b
Name: kelch domain containing 7B
Synonyms: EG546648
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 546648
Homologene: 34707
Axdnd1
Name: axonemal dynein light chain domain containing 1
Synonyms: 9430070O13Rik, LOC381304
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77352
Homologene: 52328
3425401B19Rik
Name: RIKEN cDNA 3425401B19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100504518
VEGA: 14
Homologene: 54908
Slc24a3
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 3
Synonyms: NCKX3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94249
Homologene: 41390
Meioc
Name: meiosis specific with coiled-coil domain
Synonyms: LOC268491, Gm1564, LOC380729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268491
Homologene: 19559
Parp14
Name: poly (ADP-ribose) polymerase family, member 14
Synonyms: CoaSt6, 1600029O10Rik, collaborator of Stat6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 547253
Homologene: 19697
Bmp8b
Name: bone morphogenetic protein 8b
Synonyms: Op3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12164
HGNC: HGNC:1075
Homologene: 130625
Kcna7
Name: potassium voltage-gated channel, shaker-related subfamily, member 7
Synonyms: Kv1.7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16495
HGNC: HGNC:6226
Homologene: 7791
Odr4
Name: odr4 GPCR localization factor homolog
Synonyms: BC003331, 1810053E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226499
Homologene: 41202
Cyp2d12
Name: cytochrome P450, family 2, subfamily d, polypeptide 12
Synonyms: 9030605E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 380997
VEGA: 15
Homologene: 86099
Zfp526
Name: zinc finger protein 526
Synonyms: D030024H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210172
Homologene: 18447
Rpe
Name: ribulose-5-phosphate-3-epimerase
Synonyms: 5730518J08Rik, 2810429B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66646
Homologene: 12005
Ly6g6f
Name: lymphocyte antigen 6 complex, locus G6F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 433099
Homologene: 10938
Mterf3
Name: mitochondrial transcription termination factor 3
Synonyms: 2410017I18Rik, Mterfd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66410
Homologene: 9310
Adgrg7
Name: adhesion G protein-coupled receptor G7
Synonyms: 9130020O16Rik, Gpr128
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239853
Homologene: 13115
Olfr541
Name: olfactory receptor 541
Synonyms: MOR253-3, GA_x6K02T2PBJ9-42850324-42851256
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258964
Homologene: 138322
Acad8
Name: acyl-Coenzyme A dehydrogenase family, member 8
Synonyms: 2310016C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66948
HGNC: HGNC:87
Homologene: 8662
Zfp513
Name: zinc finger protein 513
Synonyms: D430028M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 101023
Homologene: 16955
Prxl2a
Name: peroxiredoxin like 2A
Synonyms: Adiporedoxin, Adrx, 5730469M10Rik, Fam213a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70564
Homologene: 12356
Gm20427
Name: predicted gene 20427
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Gm7609
Name: predicted pseudogene 7609
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 665378
Homologene: 86179
Igkv4-63
Name: immunoglobulin kappa variable 4-63
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 628571
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 53,925,691 bp
  • G to A, chromosome 1 at 66,716,004 bp
  • G to A, chromosome 1 at 85,213,374 bp
  • A to G, chromosome 1 at 150,372,158 bp
  • A to G, chromosome 1 at 156,398,534 bp
  • A to G, chromosome 1 at 170,925,502 bp
  • A to T, chromosome 2 at 79,256,267 bp
  • C to A, chromosome 2 at 145,606,864 bp
  • C to G, chromosome 3 at 34,650,993 bp
  • T to C, chromosome 4 at 58,057,954 bp
  • T to C, chromosome 4 at 123,124,485 bp
  • G to A, chromosome 4 at 125,686,045 bp
  • A to G, chromosome 4 at 130,315,225 bp
  • A to T, chromosome 5 at 31,200,603 bp
  • C to T, chromosome 5 at 124,826,888 bp
  • G to A, chromosome 6 at 18,227,954 bp
  • G to A, chromosome 6 at 29,453,691 bp
  • T to C, chromosome 6 at 69,378,132 bp
  • T to G, chromosome 6 at 115,892,022 bp
  • A to G, chromosome 7 at 16,236,943 bp
  • T to C, chromosome 7 at 25,225,684 bp
  • G to A, chromosome 7 at 29,354,131 bp
  • A to T, chromosome 7 at 45,406,639 bp
  • G to A, chromosome 7 at 123,428,107 bp
  • A to G, chromosome 7 at 140,704,799 bp
  • A to C, chromosome 7 at 140,808,108 bp
  • A to G, chromosome 8 at 3,668,340 bp
  • C to T, chromosome 8 at 85,110,206 bp
  • A to G, chromosome 8 at 122,890,378 bp
  • C to T, chromosome 9 at 26,985,551 bp
  • T to G, chromosome 9 at 120,167,810 bp
  • C to A, chromosome 9 at 124,125,371 bp
  • G to T, chromosome 10 at 79,756,862 bp
  • A to G, chromosome 10 at 94,167,696 bp
  • A to G, chromosome 11 at 59,760,818 bp
  • A to G, chromosome 11 at 72,168,761 bp
  • A to G, chromosome 11 at 97,466,568 bp
  • G to A, chromosome 11 at 102,666,350 bp
  • T to C, chromosome 12 at 108,856,217 bp
  • A to G, chromosome 13 at 21,394,063 bp
  • A to T, chromosome 13 at 65,297,380 bp
  • C to A, chromosome 13 at 66,928,257 bp
  • T to C, chromosome 14 at 32,663,036 bp
  • T to C, chromosome 14 at 41,004,056 bp
  • T to G, chromosome 14 at 86,978,670 bp
  • C to T, chromosome 15 at 6,786,910 bp
  • G to C, chromosome 15 at 48,185,357 bp
  • G to A, chromosome 15 at 76,298,266 bp
  • A to G, chromosome 15 at 82,556,297 bp
  • A to G, chromosome 15 at 89,387,359 bp
  • C to T, chromosome 16 at 35,841,135 bp
  • T to G, chromosome 16 at 56,750,427 bp
  • A to T, chromosome 17 at 34,027,326 bp
  • A to G, chromosome 17 at 34,651,683 bp
  • A to G, chromosome 17 at 35,083,357 bp
  • C to T, chromosome 18 at 4,340,762 bp
  • G to T, chromosome 19 at 46,307,567 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5549 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043106-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.