Strain Name:
C57BL/6J-MtgxR5563Btlr/Mmmh
Stock Number:
043120-MU
Citation ID:
RRID:MMRRC_043120-MU
Other Names:
R5563 (G1), C57BL/6J-MtgxR5563Btlr
Major Collection:

Gene Information

Plin2
Name: perilipin 2
Synonyms: Adrp, adipophilin, ADPH, Adfp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11520
HGNC: HGNC:248
Homologene: 872
Prtg
Name: protogenin
Synonyms: A230098A12Rik, Igdcc5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235472
Homologene: 54453
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Topbp1
Name: topoisomerase (DNA) II binding protein 1
Synonyms: D430026L04Rik, 2810429C13Rik, 1110031N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235559
Homologene: 38262
Mars1
Name: methionine-tRNA synthetase 1
Synonyms: MetRS, methionine tRNA ligase, methionyl-tRNA synthetase, Mars
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216443
HGNC: HGNC:6898
Homologene: 90878
Jcad
Name: junctional cadherin 5 associated
Synonyms: 9430020K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240185
VEGA: 18
Homologene: 19370
Gprc5c
Name: G protein-coupled receptor, family C, group 5, member C
Synonyms: 1110028I06Rik, 3200002M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70355
Homologene: 11099
Npat
Name: nuclear protein in the AT region
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244879
HGNC: HGNC:7896
Homologene: 1888
Vps4b
Name: vacuolar protein sorting 4B
Synonyms: Skd1, 8030489C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20479
Homologene: 37976
Rhpn2
Name: rhophilin, Rho GTPase binding protein 2
Synonyms: 1300002E07Rik, D7Ertd784e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 52428
Homologene: 12407
Prkaa1
Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
Synonyms: C130083N04Rik, AMPKalpha1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105787
VEGA: 15
HGNC: HGNC:9376
Homologene: 49590
Fnip1
Name: folliculin interacting protein 1
Synonyms: A730024A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216742
Homologene: 28173
Atp7b
Name: ATPase, Cu++ transporting, beta polypeptide
Synonyms: WND, Wilson protein, Atp7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11979
HGNC: HGNC:870
Homologene: 20063
Uba5
Name: ubiquitin-like modifier activating enzyme 5
Synonyms: 5730525G14Rik, Ube1dc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66663
Homologene: 11738
Mical2
Name: microtubule associated monooxygenase, calponin and LIM domain containing 2
Synonyms: 5330438E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 320878
Homologene: 8760
Vmn2r13
Name: vomeronasal 2, receptor 13
Synonyms: Gm4867
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231589
Homologene: 129606
Sp7
Name: Sp7 transcription factor 7
Synonyms: Osx, 6430578P22Rik, osterix
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 170574
VEGA: 15
Homologene: 15607
Actn3
Name: actinin alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 11474
HGNC: HGNC:165
Homologene: 862
Psd4
Name: pleckstrin and Sec7 domain containing 4
Synonyms: SEC7 homolog, EFA6B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 215632
Homologene: 8261
Fmo5
Name: flavin containing monooxygenase 5
Synonyms: 5033418D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14263
HGNC: HGNC:3773
Homologene: 68185
Parp6
Name: poly (ADP-ribose) polymerase family, member 6
Synonyms: 2310028P13Rik, 1700119G14Rik, C030013N01Rik, 3110038K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67287
VEGA: 9
Homologene: 10627
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Fktn
Name: fukutin
Synonyms: Fukutin, Fcmd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 246179
HGNC: HGNC:3622
Homologene: 31402
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620697
Homologene: 115466
Mpp4
Name: membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4)
Synonyms: DLG6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227157
Homologene: 23296
Akp3
Name: alkaline phosphatase 3, intestine, not Mn requiring
Synonyms: IAP, Akp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11648
Homologene: 134333
Aldh5a1
Name: aldhehyde dehydrogenase family 5, subfamily A1
Synonyms: D630032B01Rik, OTTMUSG00000000613, 6330403E24Rik, SSADH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 214579
HGNC: HGNC:408
Homologene: 840
Abraxas1
Name: BRCA1 A complex subunit
Synonyms: 5630400M01Rik, 3830405G04Rik, Ccdc98, Fam175a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70681
Homologene: 16376
Mfsd4b2
Name: major facilitator superfamily domain containing 4B2
Synonyms: 2010001E11Rik, Mfsd4b2-ps, Mfsd4b2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72045
Homologene: 129931
Ighv3-1
Name: immunoglobulin heavy variable 3-1
Synonyms: Gm16610
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 780803
Gm20997
Name: predicted gene, 20997
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
1700037H04Rik
Name: RIKEN cDNA 1700037H04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67326
Homologene: 41660
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 59,124,629 bp
  • T to C, chromosome 1 at 87,125,924 bp
  • C to T, chromosome 1 at 104,947,357 bp
  • T to C, chromosome 1 at 106,779,948 bp
  • A to G, chromosome 2 at 24,394,885 bp
  • T to C, chromosome 2 at 131,147,246 bp
  • C to A, chromosome 3 at 97,638,891 bp
  • T to A, chromosome 4 at 53,761,327 bp
  • T to C, chromosome 4 at 86,662,104 bp
  • T to C, chromosome 4 at 143,134,630 bp
  • C to T, chromosome 5 at 100,812,174 bp
  • T to A, chromosome 5 at 109,173,980 bp
  • T to C, chromosome 6 at 130,503,554 bp
  • T to A, chromosome 7 at 35,371,227 bp
  • T to C, chromosome 7 at 85,222,075 bp
  • A to G, chromosome 7 at 112,314,978 bp
  • G to A, chromosome 8 at 22,028,714 bp
  • A to G, chromosome 9 at 53,563,127 bp
  • G to A, chromosome 9 at 59,628,673 bp
  • G to A, chromosome 9 at 72,856,898 bp
  • T to C, chromosome 9 at 103,311,513 bp
  • G to T, chromosome 9 at 104,049,247 bp
  • C to T, chromosome 10 at 39,922,042 bp
  • T to C, chromosome 10 at 127,308,661 bp
  • A to G, chromosome 11 at 54,504,862 bp
  • G to T, chromosome 11 at 114,864,267 bp
  • T to A, chromosome 12 at 113,964,586 bp
  • C to A, chromosome 13 at 24,918,626 bp
  • A to G, chromosome 15 at 5,169,956 bp
  • T to C, chromosome 15 at 102,359,320 bp
  • A to G, chromosome 18 at 4,673,944 bp
  • T to C, chromosome 19 at 4,872,316 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5563 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043120-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.