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Strain Name:
C57BL/6J-MtgxR5564Btlr/Mmmh
Stock Number:
043121-MU
Citation ID:
RRID:MMRRC_043121-MU
Other Names:
R5564 (G1), C57BL/6J-MtgxR5564Btlr
Major Collection:

Strain Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, Kitlg, blz
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Usp38
Name: ubiquitin specific peptidase 38
Synonyms: 4631402N15Rik, 4833420O05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74841
Homologene: 12367
Kpna2
Name: karyopherin subunit alpha 2
Synonyms: pendulin, m-importin, m-importin-alpha-P1, Rch1, 2410044B12Rik, Importin alpha, importin alpha 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16647
HGNC: HGNC:6395
Homologene: 1708
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,134,187 bp
  • A to T, chromosome 1 at 24,195,355 bp
  • G to T, chromosome 1 at 36,345,964 bp
  • C to A, chromosome 1 at 95,499,032 bp
  • T to C, chromosome 1 at 156,063,561 bp
  • C to T, chromosome 2 at 31,152,363 bp
  • T to A, chromosome 2 at 104,609,002 bp
  • A to T, chromosome 3 at 20,242,143 bp
  • T to C, chromosome 3 at 59,752,092 bp
  • A to G, chromosome 3 at 88,726,150 bp
  • A to G, chromosome 3 at 101,864,589 bp
  • C to A, chromosome 3 at 107,194,229 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • T to C, chromosome 4 at 112,988,965 bp
  • C to A, chromosome 4 at 120,468,378 bp
  • A to G, chromosome 4 at 123,526,745 bp
  • T to A, chromosome 4 at 130,053,358 bp
  • T to C, chromosome 5 at 16,312,519 bp
  • T to A, chromosome 5 at 34,420,855 bp
  • T to C, chromosome 5 at 105,014,889 bp
  • T to C, chromosome 5 at 114,389,075 bp
  • T to A, chromosome 5 at 118,742,040 bp
  • T to A, chromosome 6 at 82,981,817 bp
  • A to T, chromosome 6 at 88,013,650 bp
  • G to A, chromosome 6 at 119,948,891 bp
  • C to T, chromosome 7 at 59,995,534 bp
  • T to C, chromosome 7 at 102,670,226 bp
  • A to G, chromosome 7 at 118,189,819 bp
  • C to A, chromosome 7 at 119,971,466 bp
  • C to A, chromosome 7 at 141,356,621 bp
  • C to T, chromosome 8 at 72,571,023 bp
  • T to C, chromosome 8 at 80,985,088 bp
  • A to G, chromosome 8 at 99,030,866 bp
  • A to G, chromosome 9 at 103,334,078 bp
  • T to C, chromosome 9 at 113,812,768 bp
  • G to T, chromosome 10 at 79,978,608 bp
  • A to T, chromosome 10 at 100,080,024 bp
  • A to G, chromosome 11 at 4,148,765 bp
  • C to T, chromosome 11 at 49,957,107 bp
  • T to G, chromosome 11 at 53,478,763 bp
  • C to T, chromosome 11 at 58,831,213 bp
  • T to G, chromosome 11 at 78,191,632 bp
  • T to A, chromosome 11 at 78,201,873 bp
  • T to C, chromosome 11 at 94,430,486 bp
  • T to A, chromosome 11 at 102,538,285 bp
  • C to T, chromosome 11 at 102,800,203 bp
  • T to C, chromosome 11 at 106,990,745 bp
  • G to T, chromosome 11 at 109,934,581 bp
  • G to T, chromosome 11 at 116,849,039 bp
  • T to C, chromosome 12 at 16,824,705 bp
  • G to T, chromosome 12 at 33,048,986 bp
  • G to A, chromosome 12 at 35,124,472 bp
  • T to C, chromosome 12 at 40,124,569 bp
  • T to C, chromosome 12 at 81,419,574 bp
  • A to G, chromosome 12 at 112,963,532 bp
  • T to C, chromosome 13 at 13,995,229 bp
  • A to G, chromosome 17 at 32,775,868 bp
  • T to C, chromosome 17 at 48,317,020 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5564 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043121-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.