Strain Name:
C57BL/6J-MtgxR5584Btlr/Mmmh
Stock Number:
043138-MU
Citation ID:
RRID:MMRRC_043138-MU
Other Names:
R5584 (G1), C57BL/6J-MtgxR5584Btlr
Major Collection:

Gene Information

Epb41l4a
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: Epb4.1l4a, NBL4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13824
VEGA: 18
Homologene: 8398
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, trabeculin alpha, Aclp7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67414
Homologene: 11481
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, D230004K18Rik, Gp330, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Nsfl1c
Name: NSFL1 (p97) cofactor (p47)
Synonyms: p47
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 386649
Homologene: 41114
Etaa1
Name: Ewing tumor-associated antigen 1
Synonyms: 5730466H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68145
Homologene: 10369
Triobp
Name: TRIO and F-actin binding protein
Synonyms: EST478828, Tara, Mus EST 478828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110253
Homologene: 5104
Eprs
Name: glutamyl-prolyl-tRNA synthetase
Synonyms: Qprs, 3010002K18Rik, 2410081F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 107508
HGNC: HGNC:3418
Homologene: 5870
Ezh2
Name: enhancer of zeste 2 polycomb repressive complex 2 subunit
Synonyms: Enx-1, Enx1h, KMT6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14056
HGNC: HGNC:3527
Homologene: 37926
Nav2
Name: neuron navigator 2
Synonyms: Rainb1, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, POMFIL2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78286
Homologene: 52330
Trim27
Name: tripartite motif-containing 27
Synonyms: Rfp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19720
HGNC: HGNC:9975
Homologene: 111010
Ptprc
Name: protein tyrosine phosphatase, receptor type, C
Synonyms: T200, Lyt-4, CD45, Ly-5, B220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Pdlim1
Name: PDZ and LIM domain 1 (elfin)
Synonyms: mClim1, CLP36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 54132
HGNC: HGNC:2067
Homologene: 9643
Mbip
Name: MAP3K12 binding inhibitory protein 1
Synonyms: 4933408E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217588
VEGA: 12
Homologene: 9576
Ppip5k2
Name: diphosphoinositol pentakisphosphate kinase 2
Synonyms: Cfap160, Hisppd1, Vip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227399
Homologene: 49409
Mis18bp1
Name: MIS18 binding protein 1
Synonyms: C79407
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217653
Homologene: 10147
Znrf3
Name: zinc and ring finger 3
Synonyms: LOC382477
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 407821
Homologene: 46592
Srcap
Name: Snf2-related CREBBP activator protein
Synonyms: B930091H02Rik, F630004O05Rik, D030022P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043597
Homologene: 38213
Mir709
Name: microRNA 709
Synonyms: MIR 709, Mirn709, mmu-mir-709
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 735271
VEGA: 8
Zfpm2
Name: zinc finger protein, multitype 2
Synonyms: FOG-2, FOG2, B330005D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22762
VEGA: 15
Homologene: 8008
Fstl5
Name: follistatin-like 5
Synonyms: 9130207J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213262
Homologene: 10584
Ushbp1
Name: USH1 protein network component harmonin binding protein 1
Synonyms: MCC2, 2210404N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234395
Homologene: 12914
Sis
Name: sucrase isomaltase (alpha-glucosidase)
Synonyms: Si-s, sucrase-isomaltase, 2010204N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69983
Homologene: 37424
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Gpr98, Mgr1, Mass1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Klra13-ps
Name: killer cell lectin-like receptor subfamily A, member 13, pseudogene
Synonyms: Ly49M
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16631
Rbm46
Name: RNA binding motif protein 46
Synonyms: LOC329687, ENSMUSG00000033882
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 633285
Homologene: 17016
Sema3d
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Synonyms: 4631426B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108151
Homologene: 23664
Hivep1
Name: human immunodeficiency virus type I enhancer binding protein 1
Synonyms: alphaA-CRYBP1, Cryabp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110521
VEGA: 13
HGNC: HGNC:4920
Homologene: 1596
Flnc
Name: filamin C, gamma
Synonyms: actin binding protein 280, Fln2, 1110055E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Slc6a20a
Name: solute carrier family 6 (neurotransmitter transporter), member 20A
Synonyms: Xtrp3s1, A730081N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102680
Homologene: 10625
Efcab14
Name: EF-hand calcium binding domain 14
Synonyms: 4732418C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230648
Homologene: 8858
Lrrc49
Name: leucine rich repeat containing 49
Synonyms: D430025H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102747
Homologene: 9782
Nrg3
Name: neuregulin 3
Synonyms: ska
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18183
HGNC: HGNC:7999
Homologene: 32051
Cntn5
Name: contactin 5
Synonyms: 6720426O10Rik, A830025P08Rik, NB-2, LOC244683
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244682
HGNC: HGNC:2175
Homologene: 28447
Rabggta
Name: Rab geranylgeranyl transferase, a subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56187
VEGA: 14
HGNC: HGNC:9795
Homologene: 32443
Nlrp4e
Name: NLR family, pyrin domain containing 4E
Synonyms: 4930406H16Rik, Nalp4e, Nalp-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 446099
Homologene: 75315
Akr1c19
Name: aldo-keto reductase family 1, member C19
Synonyms: 1810010N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432720
Homologene: 134145
Thnsl1
Name: threonine synthase-like 1 (bacterial)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208967
Homologene: 32609
Plppr3
Name: phospholipid phosphatase related 3
Synonyms: Lppr3, BC005764
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216152
Homologene: 11769
Ltb4r1
Name: leukotriene B4 receptor 1
Synonyms: mBLTR, BLTR, BLT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16995
HGNC: HGNC:6713
Homologene: 22477
Gcsam
Name: germinal center associated, signaling and motility
Synonyms: M17, Gcet2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 14525
Homologene: 49157
Glrx
Name: glutaredoxin
Synonyms: D13Wsu156e, TTase, Grx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 93692
HGNC: HGNC:4330
Homologene: 37566
Unc119
Name: unc-119 lipid binding chaperone
Synonyms: Unc119h, Rtg4, HRG4, UNC119, MRG4, Rg4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22248
Homologene: 3778
Pcdha11
Name: protocadherin alpha 11
Synonyms: A830022B16Rik, Crnr7, Cnr7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12942
HGNC: HGNC:8665
Homologene: 75095
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 97,750,641 bp
  • A to T, chromosome 1 at 138,112,122 bp
  • T to C, chromosome 1 at 185,407,089 bp
  • C to T, chromosome 2 at 21,213,412 bp
  • T to C, chromosome 2 at 69,451,288 bp
  • A to G, chromosome 2 at 151,503,044 bp
  • A to G, chromosome 3 at 32,538,273 bp
  • C to G, chromosome 3 at 72,910,415 bp
  • T to A, chromosome 3 at 76,322,267 bp
  • C to A, chromosome 3 at 82,864,158 bp
  • T to C, chromosome 4 at 115,764,597 bp
  • T to C, chromosome 4 at 123,564,556 bp
  • T to C, chromosome 5 at 12,566,008 bp
  • T to C, chromosome 5 at 12,570,987 bp
  • A to G, chromosome 6 at 29,446,628 bp
  • A to T, chromosome 6 at 47,532,016 bp
  • A to G, chromosome 6 at 130,301,236 bp
  • T to G, chromosome 7 at 23,321,177 bp
  • G to T, chromosome 7 at 48,964,450 bp
  • T to A, chromosome 7 at 127,528,479 bp
  • T to C, chromosome 8 at 71,390,979 bp
  • G to A, chromosome 8 at 84,088,077 bp
  • T to C, chromosome 9 at 9,661,452 bp
  • T to A, chromosome 9 at 60,685,090 bp
  • A to G, chromosome 9 at 123,640,688 bp
  • T to C, chromosome 10 at 79,866,452 bp
  • T to C, chromosome 11 at 5,286,218 bp
  • A to T, chromosome 11 at 17,947,406 bp
  • A to G, chromosome 11 at 78,348,570 bp
  • A to G, chromosome 12 at 56,335,862 bp
  • A to T, chromosome 12 at 65,154,776 bp
  • A to T, chromosome 13 at 4,243,032 bp
  • T to A, chromosome 13 at 21,192,549 bp
  • T to A, chromosome 13 at 42,160,117 bp
  • T to A, chromosome 13 at 75,847,222 bp
  • C to T, chromosome 13 at 81,405,267 bp
  • CCCGCCGCCGCCGCCGCCGC to CCCGCCGCCGCCGCCGC, chromosome 14 at 39,472,697 bp
  • T to C, chromosome 14 at 55,720,832 bp
  • T to A, chromosome 14 at 55,767,387 bp
  • A to T, chromosome 15 at 41,102,537 bp
  • G to A, chromosome 15 at 78,968,132 bp
  • A to C, chromosome 16 at 45,619,863 bp
  • G to T, chromosome 18 at 33,854,271 bp
  • C to A, chromosome 18 at 37,006,765 bp
  • C to A, chromosome 19 at 40,223,113 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5584 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043138-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.