Strain Name:
C57BL/6J-MtgxR5589Btlr/Mmmh
Stock Number:
043142-MU
Citation ID:
RRID:MMRRC_043142-MU
Other Names:
R5589 (G1), C57BL/6J-MtgxR5589Btlr
Major Collection:

Gene Information

Thbs4
Name: thrombospondin 4
Synonyms: TSP4, TSP-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 21828
Homologene: 20691
Adcy10
Name: adenylate cyclase 10
Synonyms: sAC, Sacy, soluble adenylyl cyclase, 4930431D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 271639
Homologene: 10188
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DOXNPH, DNAPDcs, DNA-PK, slip, dxnph, XRCC7, DNA-PKcs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Smyd1
Name: SET and MYND domain containing 1
Synonyms: 4632404M21Rik, Bop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12180
Homologene: 7645
Plcd3
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72469
HGNC: HGNC:9061
Homologene: 14858
Mtmr14
Name: myotubularin related protein 14
Synonyms: 1110061O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 97287
Homologene: 11203
Serpina9
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 9
Synonyms: 2310014L03Rik, Centerin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71907
Homologene: 23633
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Ppa2
Name: pyrophosphatase (inorganic) 2
Synonyms: 1110013G13Rik, Sid6306
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74776
Homologene: 134244
Mib1
Name: mindbomb E3 ubiquitin protein ligase 1
Synonyms: skeletrophin, E430019M12Rik, mind bomb-1, Mib
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225164
Homologene: 10810
Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: Osa1, 1110030E03Rik, BAF250a, Smarcf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 93760
Homologene: 21216
Smchd1
Name: SMC hinge domain containing 1
Synonyms: MommeD1, 4931400A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74355
Homologene: 23665
Unc13d
Name: unc-13 homolog D
Synonyms: Munc13-4, Jinx, 2610108D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70450
Homologene: 26714
Ift80
Name: intraflagellar transport 80
Synonyms: Wdr56, 4921524P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68259
Homologene: 12253
Zbtb40
Name: zinc finger and BTB domain containing 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230848
Homologene: 8912
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Cntnap1
Name: contactin associated protein-like 1
Synonyms: shm, Nrxn4, Caspr, p190, NCP1, paranodin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53321
HGNC: HGNC:8011
Homologene: 2693
Uba6
Name: ubiquitin-like modifier activating enzyme 6
Synonyms: Ube1l2, 5730469D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231380
Homologene: 10080
Ccnd3
Name: cyclin D3
Synonyms: 9230106B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12445
HGNC: HGNC:1585
Homologene: 20419
Sec22b
Name: SEC22 homolog B, vesicle trafficking protein
Synonyms: 4930564D15Rik, ERS-24, Sec22l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20333
Homologene: 3597
Zfp74
Name: zinc finger protein 74
Synonyms: Zfp66, KRAB8, 2810054M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72723
Homologene: 51861
Gm14496
Name: predicted gene 14496
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 672125
Homologene: 129606
Cckbr
Name: cholecystokinin B receptor
Synonyms: CCK2/gastrin, CCK2R, CCK-B/gastrin receptor, CCKR-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12426
HGNC: HGNC:1571
Homologene: 7258
Lsg1
Name: large 60S subunit nuclear export GTPase 1
Synonyms: D16Bwg1547e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224092
Homologene: 5917
Brca2
Name: breast cancer 2, early onset
Synonyms: Fancd1, RAB163
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12190
HGNC: HGNC:1101
Homologene: 41
Serinc1
Name: serine incorporator 1
Synonyms: Tde1l, 1500011D18Rik, Tde2, TMS-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56442
Homologene: 41334
Rrbp1
Name: ribosome binding protein 1
Synonyms: 1700087N07Rik, mRRp10, 5730465C04Rik, p180, mRRp5.4, mRRp2, mRRp1.8, mRRp47, mRRp41, mRRp16.8, mRRp15b, mRRp0, ES/130, mRRp15a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 81910
Homologene: 68138
Zbtb11
Name: zinc finger and BTB domain containing 11
Synonyms: ZNF-U69274, 9230110G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 271377
Homologene: 8678
L1td1
Name: LINE-1 type transposase domain containing 1
Synonyms: ECAT11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381591
Homologene: 135709
Atp2b2
Name: ATPase, Ca++ transporting, plasma membrane 2
Synonyms: jog, wms, D6Abb2e, Tmy, Gena300, PMCA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11941
HGNC: HGNC:815
Homologene: 56150
Ttn
Name: titin
Synonyms: 2310036G12Rik, mdm, 1100001C23Rik, shru, D830007G01Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, L56, connectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408066
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: sba, 1700096C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240411
Lama3
Name: laminin, alpha 3
Synonyms: [a]3B, nicein, 150kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Pcsk6
Name: proprotein convertase subtilisin/kexin type 6
Synonyms: PACE4, b2b2830Clo, SPC4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18553
HGNC: HGNC:8569
Homologene: 20569
Haus5
Name: HAUS augmin-like complex, subunit 5
Synonyms: 2310022K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71909
Homologene: 18969
Nlrp4b
Name: NLR family, pyrin domain containing 4B
Synonyms: Nalp-gamma, Nalp4b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210045
Homologene: 65242
Vmn2r5
Name: vomeronasal 2, receptor 5
Synonyms: EG667060
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667060
Myo1c
Name: myosin IC
Synonyms: myr2, mm1beta, C80397, myosin-Ibeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17913
HGNC: HGNC:7597
Homologene: 32046
Cdh24
Name: cadherin-like 24
Synonyms: cadherin 14-like, EY-cadherin, ENSMUSG00000022188, 1700040A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239096
VEGA: 14
Homologene: 56951
Clec2e
Name: C-type lectin domain family 2, member e
Synonyms: Clra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232409
Homologene: 131164
Kctd16
Name: potassium channel tetramerisation domain containing 16
Synonyms: 4930434H12Rik, LOC383347, 2900055J20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 73001
VEGA: 18
Homologene: 44779
Usp13
Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
Synonyms: ISOT3, IsoT-3, 2700071E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72607
Homologene: 68372
Qrich2
Name: glutamine rich 2
Synonyms: LOC217341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217341
Homologene: 12951
Kif26b
Name: kinesin family member 26B
Synonyms: D230039L06Rik, N-11 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269152
Homologene: 18623
Vmn1r215
Name: vomeronasal 1 receptor 215
Synonyms: V1ri2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171253
Homologene: 110880
Olfr191
Name: olfactory receptor 191
Synonyms: MOR183-5P, GA_x54KRFPKG5P-55314632-55313703
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258035
Homologene: 128162
Spam1
Name: sperm adhesion molecule 1
Synonyms: Ph-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20690
Homologene: 7547
Mmp8
Name: matrix metallopeptidase 8
Synonyms: Collagenase-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17394
VEGA: 9
HGNC: HGNC:7175
Homologene: 22482
Ddx58
Name: DEAD/H box helicase 58
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58, 6430573D20Rik, RIG-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230073
Homologene: 32215
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228432
Homologene: 57147
Olfr1272
Name: olfactory receptor 1272
Synonyms: MOR227-3, GA_x6K02T2Q125-51636504-51635578
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258982
HGNC: HGNC:8290
Homologene: 133649
Neu3
Name: neuraminidase 3
Synonyms: membrane sialidase, ganglioside sialidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 50877
HGNC: HGNC:7760
Homologene: 4847
Ccdc68
Name: coiled-coil domain containing 68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 381175
Homologene: 11880
Olfr1154
Name: olfactory receptor 1154
Synonyms: GA_x6K02T2Q125-49403456-49402524, MOR173-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258641
Homologene: 74192
Gmnc
Name: geminin coiled-coil domain containing
Synonyms: Gm606, LOC239789, LOC385639
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239789
VEGA: 16
Homologene: 79940
Sostdc1
Name: sclerostin domain containing 1
Synonyms: Wise, ectodin, Sostl, USAG-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66042
VEGA: 12
Homologene: 9154
Olfr1095
Name: olfactory receptor 1095
Synonyms: MOR179-1, GA_x6K02T2Q125-48336843-48335917
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258725
Homologene: 28042
Gm9774
Name: predicted pseudogene 9774
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100043022
Dmgdh
Name: dimethylglycine dehydrogenase precursor
Synonyms: 1200014D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74129
Homologene: 8372
Tex47
Name: testis expressed 47
Synonyms: 4921511H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70920
Homologene: 41723
Olfr798
Name: olfactory receptor 798
Synonyms: GA_x6K02T2PULF-11304679-11303744, MOR108-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258549
Homologene: 133881
Olfr1282
Name: olfactory receptor 1282
Synonyms: Olfr1557, GA_x6K02T2Q125-72387537-72386620, MOR248-16, MOR248-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258909
Homologene: 73992
Prl3d1
Name: prolactin family 3, subfamily d, member 1
Synonyms: Csh1, prolactin-like 2, mPL-I, Pl-1, PL-Ia, Pl1, placental lactogen 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18775
Homologene: 137215
1700093K21Rik
Name: RIKEN cDNA 1700093K21 gene
Synonyms: b2b3025Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67358
Homologene: 49849
Gpt2
Name: glutamic pyruvate transaminase (alanine aminotransferase) 2
Synonyms: ALT2, 4631422C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108682
Homologene: 68832
Alg1
Name: asparagine-linked glycosylation 1 (beta-1,4-mannosyltransferase)
Synonyms: HMT1, HMAT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208211
Homologene: 5387
Klra4
Name: killer cell lectin-like receptor, subfamily A, member 4
Synonyms: Chok, ly49r129, Ly49d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16635
Homologene: 110821
Tshb
Name: thyroid stimulating hormone, beta subunit
Synonyms: thyrotropin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 22094
Homologene: 463
Cldn11
Name: claudin 11
Synonyms: oligodendrocyte-specific protein, Otm, Osp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18417
HGNC: HGNC:8514
Homologene: 4093
Lyzl4
Name: lysozyme-like 4
Synonyms: 1810009N24Rik, LYC4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69032
Homologene: 23604
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 165,539,800 bp
  • T to C, chromosome 1 at 178,916,299 bp
  • T to C, chromosome 2 at 76,768,976 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to G, chromosome 2 at 86,850,774 bp
  • T to C, chromosome 2 at 87,903,347 bp
  • C to T, chromosome 2 at 90,281,969 bp
  • A to T, chromosome 2 at 110,884,995 bp
  • T to C, chromosome 2 at 111,335,505 bp
  • T to C, chromosome 2 at 143,989,966 bp
  • C to A, chromosome 2 at 181,995,881 bp
  • A to G, chromosome 3 at 31,150,246 bp
  • T to C, chromosome 3 at 32,837,858 bp
  • T to C, chromosome 3 at 64,504,076 bp
  • T to C, chromosome 3 at 68,930,900 bp
  • T to A, chromosome 3 at 92,428,805 bp
  • T to A, chromosome 3 at 97,907,217 bp
  • A to T, chromosome 3 at 102,778,162 bp
  • C to A, chromosome 3 at 133,339,205 bp
  • T to C, chromosome 4 at 40,227,985 bp
  • C to A, chromosome 4 at 98,738,104 bp
  • T to C, chromosome 4 at 133,694,769 bp
  • T to C, chromosome 4 at 136,995,283 bp
  • T to C, chromosome 5 at 7,304,834 bp
  • G to A, chromosome 5 at 86,122,429 bp
  • T to A, chromosome 5 at 150,557,132 bp
  • C to T, chromosome 6 at 24,796,110 bp
  • C to T, chromosome 6 at 71,262,180 bp
  • T to C, chromosome 6 at 113,261,282 bp
  • T to C, chromosome 6 at 113,774,439 bp
  • T to A, chromosome 6 at 129,098,428 bp
  • G to T, chromosome 6 at 130,062,154 bp
  • G to A, chromosome 7 at 10,715,585 bp
  • A to T, chromosome 7 at 29,934,565 bp
  • A to G, chromosome 7 at 30,659,072 bp
  • T to C, chromosome 7 at 65,929,185 bp
  • G to T, chromosome 7 at 99,823,429 bp
  • T to C, chromosome 7 at 105,434,525 bp
  • A to G, chromosome 7 at 116,417,658 bp
  • A to G, chromosome 8 at 85,493,111 bp
  • T to C, chromosome 9 at 7,566,274 bp
  • C to T, chromosome 9 at 59,895,244 bp
  • G to A, chromosome 9 at 121,584,403 bp
  • C to A, chromosome 10 at 57,523,166 bp
  • T to G, chromosome 10 at 129,625,450 bp
  • T to A, chromosome 11 at 23,518,066 bp
  • A to G, chromosome 11 at 75,657,588 bp
  • A to G, chromosome 11 at 101,185,118 bp
  • T to C, chromosome 11 at 103,077,803 bp
  • A to T, chromosome 11 at 116,069,753 bp
  • C to T, chromosome 11 at 116,441,408 bp
  • C to T, chromosome 12 at 36,317,247 bp
  • G to T, chromosome 12 at 104,001,469 bp
  • A to C, chromosome 13 at 23,076,019 bp
  • G to T, chromosome 13 at 23,076,020 bp
  • T to A, chromosome 13 at 27,094,944 bp
  • T to C, chromosome 13 at 92,776,074 bp
  • T to C, chromosome 13 at 93,677,157 bp
  • A to G, chromosome 13 at 113,317,950 bp
  • G to T, chromosome 14 at 54,637,375 bp
  • T to A, chromosome 16 at 5,235,222 bp
  • C to A, chromosome 16 at 15,706,791 bp
  • T to A, chromosome 16 at 26,962,964 bp
  • T to C, chromosome 16 at 30,581,001 bp
  • T to A, chromosome 16 at 56,007,268 bp
  • A to T, chromosome 16 at 59,085,971 bp
  • G to A, chromosome 17 at 47,598,619 bp
  • A to G, chromosome 17 at 71,440,961 bp
  • A to G, chromosome 18 at 10,794,488 bp
  • C to T, chromosome 18 at 12,472,220 bp
  • C to A, chromosome 18 at 40,259,008 bp
  • G to A, chromosome 18 at 69,946,506 bp
  • T to C, chromosome 18 at 77,342,055 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5589 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043142-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.