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Strain Name:
C57BL/6J-MtgxR5589Btlr/Mmmh
Stock Number:
043142-MU
Citation ID:
RRID:MMRRC_043142-MU
Other Names:
R5589 (G1), C57BL/6J-MtgxR5589Btlr
Major Collection:

Strain Information

Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Smyd1
Name: SET and MYND domain containing 1
Synonyms: Bop, 4632404M21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12180
Homologene: 7645
Plcd3
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72469
HGNC: HGNC:9061
Homologene: 14858
Mtmr14
Name: myotubularin related protein 14
Synonyms: 1110061O04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 97287
Homologene: 11203
Serpina9
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 9
Synonyms: Centerin, 2310014L03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71907
Homologene: 23633
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Ppa2
Name: pyrophosphatase (inorganic) 2
Synonyms: Sid6306, 1110013G13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74776
Homologene: 134244
Mib1
Name: MIB E3 ubiquitin protein ligase 1
Synonyms: Mib, E430019M12Rik, skeletrophin, mind bomb-1, mindbomb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225164
Homologene: 10810
Arid1a
Name: AT-rich interaction domain 1A
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 93760
Homologene: 21216
Smchd1
Name: SMC hinge domain containing 1
Synonyms: 4931400A14Rik, MommeD1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74355
Homologene: 23665
Unc13d
Name: unc-13 homolog D
Synonyms: Munc13-4, 2610108D09Rik, Jinx
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70450
Homologene: 26714
Ift80
Name: intraflagellar transport 80
Synonyms: 4921524P20Rik, Wdr56
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68259
Homologene: 12253
Zbtb40
Name: zinc finger and BTB domain containing 40
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230848
Homologene: 8912
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Cntnap1
Name: contactin associated protein-like 1
Synonyms: p190, Caspr, Nrxn4, NCP1, paranodin, shm
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 53321
HGNC: HGNC:8011
Homologene: 2693
Uba6
Name: ubiquitin-like modifier activating enzyme 6
Synonyms: 5730469D23Rik, Ube1l2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231380
Homologene: 10080
Ccnd3
Name: cyclin D3
Synonyms: 9230106B05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12445
HGNC: HGNC:1585
Homologene: 20419
Sec22b
Name: SEC22 homolog B, vesicle trafficking protein
Synonyms: ERS-24, 4930564D15Rik, Sec22l1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20333
Homologene: 3597
Zfp74
Name: zinc finger protein 74
Synonyms: Zfp66, KRAB8, 2810054M15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72723
Homologene: 51861
Gm14496
Name: predicted gene 14496
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 672125
Homologene: 129606
Cckbr
Name: cholecystokinin B receptor
Synonyms: CCK-B/gastrin receptor, CCK2/gastrin, CCK2R, CCKR-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12426
HGNC: HGNC:1571
Homologene: 7258
Lsg1
Name: large 60S subunit nuclear export GTPase 1
Synonyms: D16Bwg1547e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224092
Homologene: 5917
Brca2
Name: breast cancer 2, early onset
Synonyms: RAB163, Fancd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12190
HGNC: HGNC:1101
Homologene: 41
Serinc1
Name: serine incorporator 1
Synonyms: TMS-2, 1500011D18Rik, Tde1l, Tde2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 56442
Homologene: 41334
Rrbp1
Name: ribosome binding protein 1
Synonyms: mRRp0, ES/130, p180, mRRp1.8, mRRp2, mRRp5.4, mRRp10, mRRp16.8, mRRp15b, mRRp15a, mRRp41, mRRp47, 1700087N07Rik, 5730465C04Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 81910
Homologene: 68138
Zbtb11
Name: zinc finger and BTB domain containing 11
Synonyms: ZNF-U69274, 9230110G02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 271377
Homologene: 8678
L1td1
Name: LINE-1 type transposase domain containing 1
Synonyms: ECAT11
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381591
Homologene: 135709
Atp2b2
Name: ATPase, Ca++ transporting, plasma membrane 2
Synonyms: PMCA2, D6Abb2e, wms, jog, Tmy, Gena300
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11941
HGNC: HGNC:815
Homologene: 56150
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 408066
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240411
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Pcsk6
Name: proprotein convertase subtilisin/kexin type 6
Synonyms: PACE4, SPC4, b2b2830Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18553
HGNC: HGNC:8569
Homologene: 20569
Haus5
Name: HAUS augmin-like complex, subunit 5
Synonyms: 2310022K01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71909
Homologene: 18969
Nlrp4b
Name: NLR family, pyrin domain containing 4B
Synonyms: Nalp-gamma, Nalp4b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210045
Homologene: 65242
Vmn2r5
Name: vomeronasal 2, receptor 5
Synonyms: EG667060
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 667060
Myo1c
Name: myosin IC
Synonyms: myosin-Ibeta, myr2, C80397, mm1beta
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17913
HGNC: HGNC:7597
Homologene: 32046
Cdh24
Name: cadherin-like 24
Synonyms: cadherin 14-like, EY-cadherin, 1700040A22Rik, ENSMUSG00000022188
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239096
VEGA: 14
Homologene: 56951
Clec2e
Name: C-type lectin domain family 2, member e
Synonyms: Clra
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232409
Homologene: 131164
Kctd16
Name: potassium channel tetramerisation domain containing 16
Synonyms: 4930434H12Rik, LOC383347, 2900055J20Rik, C030017B01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 383348
VEGA: 18
Homologene: 44779
Usp13
Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
Synonyms: IsoT-3, ISOT3, 2700071E21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72607
Homologene: 68372
Qrich2
Name: glutamine rich 2
Synonyms: LOC217341
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217341
Homologene: 12951
Kif26b
Name: kinesin family member 26B
Synonyms: N-11 kinesin, D230039L06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269152
Homologene: 18623
Vmn1r215
Name: vomeronasal 1 receptor 215
Synonyms: V1ri2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171253
Homologene: 110880
Or5h23
Name: olfactory receptor family 5 subfamily H member 23
Synonyms: GA_x54KRFPKG5P-55314632-55313703, MOR183-5P, Olfr191
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258035
Homologene: 128162
Spam1
Name: sperm adhesion molecule 1
Synonyms: Ph-20
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20690
Homologene: 7547
Mmp8
Name: matrix metallopeptidase 8
Synonyms: Collagenase-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17394
VEGA: 9
HGNC: HGNC:7175
Homologene: 22482
Rigi
Name: RNA sensor RIG-I
Synonyms: 6430573D20Rik, RIG-I, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58, Ddx58
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230073
Homologene: 32215
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228432
Homologene: 57147
Or4b1b
Name: olfactory receptor family 4 subfamily B member 1B
Synonyms: GA_x6K02T2Q125-51636504-51635578, MOR227-3, Olfr1272
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258982
HGNC: HGNC:8290
Homologene: 133649
Neu3
Name: neuraminidase 3
Synonyms: membrane sialidase, ganglioside sialidase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50877
HGNC: HGNC:7760
Homologene: 4847
Ccdc68
Name: coiled-coil domain containing 68
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 381175
Homologene: 11880
Or9m1
Name: olfactory receptor family 9 subfamily M member 1
Synonyms: GA_x6K02T2Q125-49403456-49402524, MOR173-2, Olfr1154
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258641
Homologene: 74192
Gmnc
Name: geminin coiled-coil domain containing
Synonyms: LOC239789, LOC385639, Gm606
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239789
VEGA: 16
Homologene: 79940
Sostdc1
Name: sclerostin domain containing 1
Synonyms: Sostl, ectodin, USAG-1, Wise
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66042
VEGA: 12
Homologene: 9154
Or5t15
Name: olfactory receptor family 5 subfamily T member 15
Synonyms: GA_x6K02T2Q125-48336843-48335917, MOR179-1, Olfr1095
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258725
Homologene: 28042
Adrm1b
Name: adhesion regulating molecule 1B
Synonyms: Gm9774
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100043022
Dmgdh
Name: dimethylglycine dehydrogenase precursor
Synonyms: 1200014D15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 74129
Homologene: 8372
Tex47
Name: testis expressed 47
Synonyms: 4921511H03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70920
Homologene: 41723
Or6c66
Name: olfactory receptor family 6 subfamily C member 66
Synonyms: GA_x6K02T2PULF-11304679-11303744, MOR108-1, Olfr798
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258549
Homologene: 133881
Or4k38
Name: olfactory receptor family 4 subfamily K member 38
Synonyms: GA_x6K02T2Q125-72387537-72386620, MOR248-16, MOR248-2, Olfr1557, Olfr1282
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258909
Homologene: 73992
Prl3d1
Name: prolactin family 3, subfamily d, member 1
Synonyms: Pl-1, Pl1, prolactin-like 2, PL-Ia, Csh1, mPL-I, placental lactogen 1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18775
Homologene: 137215
1700093K21Rik
Name: RIKEN cDNA 1700093K21 gene
Synonyms: b2b3025Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67358
Homologene: 49849
Gpt2
Name: glutamic pyruvate transaminase (alanine aminotransferase) 2
Synonyms: ALT2, 4631422C05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108682
Homologene: 68832
Alg1
Name: ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase
Synonyms: HMT1, HMAT1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208211
Homologene: 5387
Klra4
Name: killer cell lectin-like receptor, subfamily A, member 4
Synonyms: Chok, Ly49d, ly49r129
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16635
Homologene: 110821
Tshb
Name: thyroid stimulating hormone, beta subunit
Synonyms: thyrotropin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 22094
Homologene: 463
Cldn11
Name: claudin 11
Synonyms: oligodendrocyte-specific protein, Otm, Osp
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18417
HGNC: HGNC:8514
Homologene: 4093
Lyzl4
Name: lysozyme-like 4
Synonyms: LYC4, 1810009N24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69032
Homologene: 23604
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 165,539,800 bp
  • T to C, chromosome 1 at 178,916,299 bp
  • T to C, chromosome 2 at 76,768,976 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to G, chromosome 2 at 86,850,774 bp
  • T to C, chromosome 2 at 87,903,347 bp
  • C to T, chromosome 2 at 90,281,969 bp
  • A to T, chromosome 2 at 110,884,995 bp
  • T to C, chromosome 2 at 111,335,505 bp
  • T to C, chromosome 2 at 143,989,966 bp
  • C to A, chromosome 2 at 181,995,881 bp
  • A to G, chromosome 3 at 31,150,246 bp
  • T to C, chromosome 3 at 32,837,858 bp
  • T to C, chromosome 3 at 64,504,076 bp
  • T to C, chromosome 3 at 68,930,900 bp
  • T to A, chromosome 3 at 92,428,805 bp
  • T to A, chromosome 3 at 97,907,217 bp
  • A to T, chromosome 3 at 102,778,162 bp
  • C to A, chromosome 3 at 133,339,205 bp
  • T to C, chromosome 4 at 40,227,985 bp
  • C to A, chromosome 4 at 98,738,104 bp
  • T to C, chromosome 4 at 133,694,769 bp
  • T to C, chromosome 4 at 136,995,283 bp
  • T to C, chromosome 5 at 7,304,834 bp
  • G to A, chromosome 5 at 86,122,429 bp
  • T to A, chromosome 5 at 150,557,132 bp
  • C to T, chromosome 6 at 24,796,110 bp
  • C to T, chromosome 6 at 71,262,180 bp
  • T to C, chromosome 6 at 113,261,282 bp
  • T to C, chromosome 6 at 113,774,439 bp
  • T to A, chromosome 6 at 129,098,428 bp
  • G to T, chromosome 6 at 130,062,154 bp
  • G to A, chromosome 7 at 10,715,585 bp
  • A to T, chromosome 7 at 29,934,565 bp
  • A to G, chromosome 7 at 30,659,072 bp
  • T to C, chromosome 7 at 65,929,185 bp
  • G to T, chromosome 7 at 99,823,429 bp
  • T to C, chromosome 7 at 105,434,525 bp
  • A to G, chromosome 7 at 116,417,658 bp
  • A to G, chromosome 8 at 85,493,111 bp
  • T to C, chromosome 9 at 7,566,274 bp
  • C to T, chromosome 9 at 59,895,244 bp
  • G to A, chromosome 9 at 121,584,403 bp
  • C to A, chromosome 10 at 57,523,166 bp
  • T to G, chromosome 10 at 129,625,450 bp
  • T to A, chromosome 11 at 23,518,066 bp
  • A to G, chromosome 11 at 75,657,588 bp
  • A to G, chromosome 11 at 101,185,118 bp
  • T to C, chromosome 11 at 103,077,803 bp
  • A to T, chromosome 11 at 116,069,753 bp
  • C to T, chromosome 11 at 116,441,408 bp
  • C to T, chromosome 12 at 36,317,247 bp
  • G to T, chromosome 12 at 104,001,469 bp
  • A to C, chromosome 13 at 23,076,019 bp
  • G to T, chromosome 13 at 23,076,020 bp
  • T to A, chromosome 13 at 27,094,944 bp
  • T to C, chromosome 13 at 92,776,074 bp
  • T to C, chromosome 13 at 93,677,157 bp
  • A to G, chromosome 13 at 113,317,950 bp
  • G to T, chromosome 14 at 54,637,375 bp
  • T to A, chromosome 16 at 5,235,222 bp
  • C to A, chromosome 16 at 15,706,791 bp
  • T to A, chromosome 16 at 26,962,964 bp
  • T to C, chromosome 16 at 30,581,001 bp
  • T to A, chromosome 16 at 56,007,268 bp
  • A to T, chromosome 16 at 59,085,971 bp
  • G to A, chromosome 17 at 47,598,619 bp
  • A to G, chromosome 17 at 71,440,961 bp
  • A to G, chromosome 18 at 10,794,488 bp
  • C to T, chromosome 18 at 12,472,220 bp
  • C to A, chromosome 18 at 40,259,008 bp
  • G to A, chromosome 18 at 69,946,506 bp
  • T to C, chromosome 18 at 77,342,055 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5589 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043142-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.