Strain Name:
C57BL/6J-MtgxR5616Btlr/Mmmh
Stock Number:
043159-MU
Citation ID:
RRID:MMRRC_043159-MU
Other Names:
R5616 (G1), C57BL/6J-MtgxR5616Btlr
Major Collection:

Strain Information

Wnt3
Name: wingless-type MMTV integration site family, member 3
Synonyms: Wnt-3, Int-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22415
Homologene: 22527
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: alpha13.2, Cav3.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Pelp1
Name: proline, glutamic acid and leucine rich protein 1
Synonyms: 4930563C04Rik, MNAR
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75273
Homologene: 8664
Larp4b
Name: La ribonucleoprotein 4B
Synonyms: D13Wsu64e, Larp5, A130023E24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 217980
Homologene: 18195
Ptprg
Name: protein tyrosine phosphatase receptor type G
Synonyms: 5430405N12Rik, RPTPgamma
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Fkbp4
Name: FK506 binding protein 4
Synonyms: FKBP-52, FKBP52
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14228
HGNC: HGNC:3720
Homologene: 36085
Cenpf
Name: centromere protein F
Synonyms: 6530404A22Rik, Lek1, mitosin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108000
HGNC: HGNC:1857
Homologene: 22969
Dhx32
Name: DEAH-box helicase 32 (putative)
Synonyms: Ddx32, DEAH (Asp-Glu-Ala-His) box polypeptide 32
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101437
Homologene: 56798
Abcd3
Name: ATP-binding cassette, sub-family D member 3
Synonyms: PMP70, Pxmp1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19299
HGNC: HGNC:67
Homologene: 2140
Lrrc8a
Name: leucine rich repeat containing 8A VRAC subunit A
Synonyms: Lrrc8, ebo, SWELL1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241296
Homologene: 18617
Cul1
Name: cullin 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26965
HGNC: HGNC:2551
Homologene: 2663
Dcakd
Name: dephospho-CoA kinase domain containing
Synonyms: 3010024O21Rik, 6720485C15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68087
Homologene: 5652
Tsc22d2
Name: TSC22 domain family, member 2
Synonyms: 1810043J12Rik, 5530402M19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72033
Homologene: 8860
Nipal3
Name: NIPA-like domain containing 3
Synonyms: 9130020G22Rik, Npal3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74552
Homologene: 10722
Mplkipl1
Name: M-phase specific PLK1 intereacting protein like 1
Synonyms: Gm7102
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 633057
Nfkb2
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
Synonyms: p52, NF kappaB2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18034
VEGA: 19
HGNC: HGNC:7795
Homologene: 1873
Dppa4
Name: developmental pluripotency associated 4
Synonyms: 2410091M23Rik, ECAT15-1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 73693
VEGA: 16
Homologene: 130462
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alpha myosin, Myhc-a, alpha cardiac MHC, cardiomyopathy, hypertrophic 1, Myhca, A830009F23Rik, alpha-MHC, alphaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Klhl1
Name: kelch-like 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 93688
HGNC: HGNC:6352
Homologene: 56903
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240411
Srp72
Name: signal recognition particle 72
Synonyms: 72kDa, 5730576P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66661
Homologene: 38254
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: EG640524, Spnb5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 640524
Homologene: 41150
Cct6b
Name: chaperonin containing TCP1 subunit 6B
Synonyms: CCTzeta-2, Cctz-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12467
HGNC: HGNC:1621
Homologene: 55978
Vmn2r59
Name: vomeronasal 2, receptor 59
Synonyms: EG628444
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 628444
Homologene: 129683
Matn3
Name: matrilin 3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17182
HGNC: HGNC:6909
Homologene: 1785
Rtl1
Name: retrotransposon Gaglike 1
Synonyms: Mart1, Mor1, Mar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 353326
VEGA: 12
Homologene: 120261
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cav2.3, Cchra1, alpha1E
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
B3glct
Name: beta-3-glucosyltransferase
Synonyms: LOC381694, B3galtl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381694
Homologene: 14978
Mtrf1
Name: mitochondrial translational release factor 1
Synonyms: A830062K05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211253
VEGA: 14
HGNC: HGNC:7469
Homologene: 20903
Trim41
Name: tripartite motif-containing 41
Synonyms: RINCK
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211007
Homologene: 14140
Fam98b
Name: family with sequence similarity 98, member B
Synonyms: 2610510H03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68215
Homologene: 72241
Syne3
Name: spectrin repeat containing, nuclear envelope family member 3
Synonyms: nesprin-3beta, nesprin-3alpha, nesprin-3, 4831426I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 212073
VEGA: 12
Homologene: 17625
Mios
Name: meiosis regulator for oocyte development
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 252875
Homologene: 10371
Tgm2
Name: transglutaminase 2, C polypeptide
Synonyms: tissue transglutaminase, protein-glutamine gamma-glutamyltransferase, G[a]h, TG C, tTGas, tTG, TG2, TGase2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21817
Homologene: 3391
Pcdhb20
Name: protocadherin beta 20
Synonyms: Pcdhb14, PcdhbT
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93891
HGNC: HGNC:8685
Homologene: 134303
Gpr33
Name: G protein-coupled receptor 33
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14762
HGNC: HGNC:4489
Homologene: 7345
Disp1
Name: dispatched RND transporter family member 1
Synonyms: DispA, 1190008H24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68897
Homologene: 14133
Vmn1r42
Name: vomeronasal 1 receptor 42
Synonyms: V1ra6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113848
Homologene: 130651
Faap20
Name: Fanconi anemia core complex associated protein 20
Synonyms: 2610002J02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67513
Homologene: 18793
Gm38393
Name: predicted gene, 38393
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Gpr31b
Name: G protein-coupled receptor 31, D17Leh66b region
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 436440
VEGA: 17
HGNC: HGNC:4486
Homologene: 48337
RP23-371A16.4
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Gm26752
Name: predicted gene, 26752
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 102631579
VEGA: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 154,442,194 bp
  • T to A, chromosome 1 at 183,088,349 bp
  • A to G, chromosome 1 at 189,657,286 bp
  • T to C, chromosome 2 at 30,255,354 bp
  • A to G, chromosome 2 at 117,267,786 bp
  • G to A, chromosome 2 at 120,046,484 bp
  • A to G, chromosome 2 at 158,128,720 bp
  • G to T, chromosome 3 at 58,417,162 bp
  • A to T, chromosome 3 at 121,772,360 bp
  • T to C, chromosome 4 at 135,452,404 bp
  • C to T, chromosome 4 at 155,256,248 bp
  • T to G, chromosome 5 at 76,987,934 bp
  • C to A, chromosome 5 at 123,111,839 bp
  • T to A, chromosome 5 at 149,729,934 bp
  • C to T, chromosome 6 at 8,226,822 bp
  • G to A, chromosome 6 at 47,523,788 bp
  • T to C, chromosome 6 at 89,845,102 bp
  • A to T, chromosome 6 at 128,433,554 bp
  • A to G, chromosome 7 at 42,058,767 bp
  • C to T, chromosome 7 at 59,999,385 bp
  • T to C, chromosome 7 at 133,721,228 bp
  • G to A, chromosome 10 at 3,366,957 bp
  • GCCTAGGCGCCCA to G, chromosome 11 at 48,807,365 bp
  • G to A, chromosome 11 at 70,394,862 bp
  • A to T, chromosome 11 at 82,741,349 bp
  • A to G, chromosome 11 at 102,995,005 bp
  • T to C, chromosome 11 at 103,812,770 bp
  • A to G, chromosome 12 at 8,948,195 bp
  • T to C, chromosome 12 at 52,023,594 bp
  • T to C, chromosome 12 at 104,955,678 bp
  • C to T, chromosome 12 at 109,592,739 bp
  • T to A, chromosome 13 at 9,158,659 bp
  • A to G, chromosome 14 at 12,122,120 bp
  • T to C, chromosome 14 at 54,956,581 bp
  • T to C, chromosome 14 at 79,401,445 bp
  • A to G, chromosome 14 at 96,518,293 bp
  • T to A, chromosome 16 at 48,291,030 bp
  • A to G, chromosome 17 at 13,051,944 bp
  • C to A, chromosome 17 at 25,377,667 bp
  • A to G, chromosome 18 at 37,504,532 bp
  • T to C, chromosome 18 at 77,404,951 bp
  • G to T, chromosome 19 at 46,307,567 bp
  • C to T, chromosome 19 at 61,175,926 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5616 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043159-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.