Strain Name:
C57BL/6J-MtgxR5648Btlr/Mmmh
Stock Number:
043169-MU
Citation ID:
RRID:MMRRC_043169-MU
Other Names:
R5648 (G1), C57BL/6J-MtgxR5648Btlr
Major Collection:

Gene Information

Nefh
Name: neurofilament, heavy polypeptide
Synonyms: NF-H, NF200, NEFH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380684
HGNC: HGNC:7737
Homologene: 40755
Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Esco2
Name: establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms: D030072L07Rik, 2410004I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71988
Homologene: 12432
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Rps6kb1
Name: ribosomal protein S6 kinase, polypeptide 1
Synonyms: p70/85s6k, p70s6k, S6K1, 2610318I15Rik, p70S6K1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72508
Homologene: 81703
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Dnmt3b
Name: DNA methyltransferase 3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13436
HGNC: HGNC:2979
Homologene: 56000
Fancl
Name: Fanconi anemia, complementation group L
Synonyms: 2010322C19Rik, gcd, Pog, Phf9, B230118H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67030
Homologene: 9987
Thoc1
Name: THO complex 1
Synonyms: 3110002N20Rik, NMP-84
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225160
VEGA: 18
Homologene: 38012
Slc12a2
Name: solute carrier family 12, member 2
Synonyms: sodium/potassium/chloride cotransporters, mBSC2, Nkcc1, sy-ns
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20496
VEGA: 18
Homologene: 20283
Cald1
Name: caldesmon 1
Synonyms: 4833423D12Rik, C920027I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 109624
HGNC: HGNC:1441
Homologene: 137254
Ccser2
Name: coiled-coil serine rich 2
Synonyms: 1700012P13Rik, 2900054P12Rik, Gcap14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72972
Homologene: 10367
Ticam1
Name: toll-like receptor adaptor molecule 1
Synonyms: TICAM-1, Trif
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106759
Homologene: 8605
Klk8
Name: kallikrein related-peptidase 8
Synonyms: BSP1, Prss19, Nrpn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259277
HGNC: HGNC:6369
Homologene: 21410
Ncr1
Name: natural cytotoxicity triggering receptor 1
Synonyms: NKp46, MAR1 (mouse activating receptor 1), Ly94
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17086
HGNC: HGNC:6731
Homologene: 3551
Gm9847
Name: predicted pseudogene 9847
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100043256
VEGA: 12
Pcdhb18
Name: protocadherin beta 18
Synonyms: Pcdhb9, PcdhbR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93889
Homologene: 137649
Akap2
Name: A kinase (PRKA) anchor protein 2
Synonyms: B230340M18Rik, AKAP-KL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Gnao1
Name: guanine nucleotide binding protein, alpha O
Synonyms: Go alpha, alphaO, Galphao
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14681
HGNC: HGNC:4389
Homologene: 39203
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Ttll7
Name: tubulin tyrosine ligase-like family, member 7
Synonyms: 1110049N09Rik, 4921517B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70892
Homologene: 41578
Wdr93
Name: WD repeat domain 93
Synonyms: EG626359
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 626359
Homologene: 56878
Vmn1r78
Name: vomeronasal 1 receptor 78
Synonyms: V1rg7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171242
Homologene: 74316
Olfr1253
Name: olfactory receptor 1253
Synonyms: GA_x6K02T2Q125-51193814-51192857, MOR231-19P, MOR231-19P, MOR231-18, Olfr1253-ps1, Olfr1559-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258370
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Rhobtb2
Name: Rho-related BTB domain containing 2
Synonyms: Dbc2, E130206H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 246710
VEGA: 14
Homologene: 22873
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: 6530405F15Rik, CMF608, Adlican2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242050
Homologene: 18712
Ggt6
Name: gamma-glutamyltransferase 6
Synonyms: 9030405D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71522
Homologene: 51918
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225638
Homologene: 50475
Map3k6
Name: mitogen-activated protein kinase kinase kinase 6
Synonyms: Ask2, MEKK6, MAPKKK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53608
HGNC: HGNC:6858
Homologene: 3435
Abca2
Name: ATP-binding cassette, sub-family A (ABC1), member 2
Synonyms: Abc2, D2H0S1474E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11305
HGNC: HGNC:32
Homologene: 55590
Zfp983
Name: zinc finger protein 983
Synonyms: 3110052M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 73229
VEGA: 17
Itih3
Name: inter-alpha trypsin inhibitor, heavy chain 3
Synonyms: Intin3, Itih-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16426
HGNC: HGNC:6168
Homologene: 1669
Plekhd1
Name: pleckstrin homology domain containing, family D (with coiled-coil domains) member 1
Synonyms: 3830431G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217682
Homologene: 15090
Hyou1
Name: hypoxia up-regulated 1
Synonyms: CBP-140, Orp150, 140 kDa, Grp170, Cab140
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12282
Homologene: 4658
Tmem117
Name: transmembrane protein 117
Synonyms: B930062P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320709
VEGA: 15
Homologene: 12992
Hyal6
Name: hyaluronoglucosaminidase 6
Synonyms: 4932701A20Rik, Hyal-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74409
HGNC: HGNC:5324
Homologene: 78028
Zhx3
Name: zinc fingers and homeoboxes 3
Synonyms: 4932418O04Rik, 1810059C13Rik, 9530010N21Rik, Tix1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320799
Homologene: 9000
Ubqln3
Name: ubiquilin 3
Synonyms: 4933400K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244178
Homologene: 9700
Ubd
Name: ubiquitin D
Synonyms: FAT10, Diubiquitin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 24108
Homologene: 4665
Slc4a1ap
Name: solute carrier family 4 (anion exchanger), member 1, adaptor protein
Synonyms: kanadaptin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20534
Homologene: 7543
Gm10392
Name: predicted gene 10392
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Gvin1
Name: GTPase, very large interferon inducible 1
Synonyms: VLIG-1, 9830104F22Rik, VLIG, 9130002C22Rik, Iigs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74558
Homologene: 32708
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 20,558,626 bp
  • T to C, chromosome 1 at 77,398,525 bp
  • T to C, chromosome 2 at 25,436,498 bp
  • A to C, chromosome 2 at 89,752,073 bp
  • G to A, chromosome 2 at 153,677,198 bp
  • A to T, chromosome 2 at 160,781,961 bp
  • G to C, chromosome 3 at 59,328,153 bp
  • A to G, chromosome 3 at 146,961,710 bp
  • T to C, chromosome 4 at 57,854,848 bp
  • A to C, chromosome 4 at 133,243,335 bp
  • A to G, chromosome 5 at 21,998,572 bp
  • A to G, chromosome 5 at 31,550,785 bp
  • T to C, chromosome 6 at 24,734,236 bp
  • G to T, chromosome 6 at 34,762,332 bp
  • T to A, chromosome 7 at 4,344,520 bp
  • G to A, chromosome 7 at 12,152,766 bp
  • T to C, chromosome 7 at 43,798,644 bp
  • T to C, chromosome 7 at 79,777,226 bp
  • CCAGGGC to CC, chromosome 7 at 80,098,339 bp
  • A to G, chromosome 7 at 104,140,910 bp
  • A to T, chromosome 7 at 106,163,399 bp
  • G to A, chromosome 7 at 134,746,954 bp
  • A to T, chromosome 8 at 93,949,442 bp
  • T to A, chromosome 9 at 44,385,249 bp
  • T to C, chromosome 10 at 62,616,270 bp
  • A to T, chromosome 11 at 4,945,233 bp
  • T to C, chromosome 11 at 26,422,459 bp
  • A to T, chromosome 11 at 65,927,755 bp
  • T to C, chromosome 11 at 72,435,716 bp
  • A to T, chromosome 11 at 77,517,480 bp
  • T to C, chromosome 11 at 86,512,871 bp
  • A to G, chromosome 12 at 14,495,129 bp
  • T to C, chromosome 12 at 80,720,588 bp
  • T to C, chromosome 14 at 30,921,418 bp
  • A to T, chromosome 14 at 36,878,945 bp
  • A to T, chromosome 14 at 65,831,192 bp
  • G to T, chromosome 14 at 69,797,144 bp
  • T to A, chromosome 14 at 103,291,342 bp
  • T to G, chromosome 15 at 95,094,772 bp
  • T to A, chromosome 17 at 21,659,031 bp
  • T to A, chromosome 17 at 37,195,454 bp
  • TCACACA to TCACA, chromosome 17 at 56,270,629 bp
  • A to G, chromosome 18 at 9,962,390 bp
  • G to A, chromosome 18 at 37,490,484 bp
  • G to A, chromosome 18 at 57,896,310 bp
  • A to G, chromosome 18 at 65,349,917 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5648 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043169-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.