Strain Name:
C57BL/6J-MtgxR5727Btlr/Mmmh
Stock Number:
043190-MU
Citation ID:
RRID:MMRRC_043190-MU
Other Names:
R5727 (G1), C57BL/6J-MtgxR5727Btlr
Major Collection:

Gene Information

Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Ptprf
Name: protein tyrosine phosphatase, receptor type, F
Synonyms: RPTP-LAR, LAR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Phf12
Name: PHD finger protein 12
Synonyms: 2410142K10Rik, PF1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 268448
Homologene: 10841
Cdyl2
Name: chromodomain protein, Y chromosome-like 2
Synonyms: 1700029M19Rik, 4930453I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 75796
Homologene: 41779
Mthfd1l
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms: 2410004L15Rik, Fthfsdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 270685
Homologene: 56706
Ube2o
Name: ubiquitin-conjugating enzyme E2O
Synonyms: B230113M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 217342
Homologene: 11113
Ttc39b
Name: tetratricopeptide repeat domain 39B
Synonyms: 1810054D07Rik, 9130422G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69863
Homologene: 25228
Gnb1
Name: guanine nucleotide binding protein (G protein), beta 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14688
HGNC: HGNC:4396
Homologene: 55532
Snx5
Name: sorting nexin 5
Synonyms: D2Ertd52e, 1810032P22Rik, 0910001N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 69178
Homologene: 40944
Mdm2
Name: transformed mouse 3T3 cell double minute 2
Synonyms: 1700007J15Rik, Mdm-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 17246
HGNC: HGNC:6973
Homologene: 1793
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 72745
Homologene: 14519
Nkiras2
Name: NFKB inhibitor interacting Ras-like protein 2
Synonyms: 2410003M04Rik, 4930527H08Rik, KBRAS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 71966
Homologene: 9738
Actr8
Name: ARP8 actin-related protein 8
Synonyms: ARP8, 5730542K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 56249
VEGA: 14
Homologene: 11286
Ehmt2
Name: euchromatic histone lysine N-methyltransferase 2
Synonyms: G9a, NG36, D17Ertd710e, Bat8, KMT1C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 110147
Homologene: 48460
Hyls1
Name: HYLS1, centriolar and ciliogenesis associated
Synonyms: 3010015K02Rik, hydrolethalus syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 76832
Homologene: 82283
Sptb
Name: spectrin beta, erythrocytic
Synonyms: spectrin R, brain erythroid spectrin (235E), LOC383567, Spnb-1, Spnb1, D330027P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20741
Homologene: 295
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72205
Homologene: 8125
Phactr4
Name: phosphatase and actin regulator 4
Synonyms: C330013F19Rik, 3110001B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100169
Homologene: 41537
Vwa3b
Name: von Willebrand factor A domain containing 3B
Synonyms: A230074B11Rik, 4921511C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70853
Homologene: 27053
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1289Clo, b2b1727Clo, b2b598Clo, lrd, b2b1203Clo, b2b1279Clo, avc4, Dnahc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Oc90
Name: otoconin 90
Synonyms: PLA2L, Ocn-95, Pla2ll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 18256
HGNC: HGNC:8100
Homologene: 19021
Gm1966
Name: predicted gene 1966
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434223
Homologene: 32708
Cfap44
Name: cilia and flagella associated protein 44
Synonyms: 6330444M21Rik, D16Ertd642e, Wdr52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 212517
Homologene: 75085
Vmn2r2
Name: vomeronasal 2, receptor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 100125586
Homologene: 129753
Ppfia4
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Synonyms: Gm3812, Liprin-alpha4, 1110008G13Rik, LOC100042382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 68507
HGNC: HGNC:9248
Homologene: 66200
Cdhr2
Name: cadherin-related family member 2
Synonyms: Pcdh24, LOC268663
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 268663
Homologene: 134510
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: 1110004P15Rik, 2310047C17Rik, DY6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Micall1
Name: microtubule associated monooxygenase, calponin and LIM domain containing -like 1
Synonyms: D15Mit260, D15N2e, Mus EST 820961
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 27008
VEGA: 15
Homologene: 24911
Gm10845
Name: predicted gene 10845
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 100038734
VEGA: 14
Parp9
Name: poly (ADP-ribose) polymerase family, member 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 80285
Homologene: 12720
Olfr1277
Name: olfactory receptor 1277
Synonyms: GA_x6K02T2Q125-72321818-72320907, MOR248-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258391
Homologene: 105176
Kif21b
Name: kinesin family member 21B
Synonyms: 2610511N21Rik, N-5 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16565
Homologene: 56868
Sorcs2
Name: sortilin-related VPS10 domain containing receptor 2
Synonyms: VPS10 domain receptor protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 81840
Homologene: 56899
Olfr350
Name: olfactory receptor 350
Synonyms: GA_x6K02T2NLDC-33544602-33545540, MOR136-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258620
HGNC: HGNC:8211
Homologene: 64897
Cpxm1
Name: carboxypeptidase X 1 (M14 family)
Synonyms: Cpx-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56264
Homologene: 10485
Stom
Name: stomatin
Synonyms: stomatin, Epb7.2, protein 7.2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13830
HGNC: HGNC:3383
Homologene: 81681
Wscd2
Name: WSC domain containing 2
Synonyms: C530024P05Rik, 4933413A10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 320916
Homologene: 35372
Pex13
Name: peroxisomal biogenesis factor 13
Synonyms: 2610008O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 72129
HGNC: HGNC:8855
Homologene: 1967
Olfr748
Name: olfactory receptor 748
Synonyms: MOR106-9P, GA_x6K02T2PMLR-6454789-6455712
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258113
Homologene: 134081
Rragc
Name: Ras-related GTP binding C
Synonyms: Gtr2, YGR163W, TIB929
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 54170
Homologene: 39141
Zfp414
Name: zinc finger protein 414
Synonyms: 0610030H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328801
Homologene: 12264
Olfr12
Name: olfactory receptor 12
Synonyms: MOR208-5, GA_x6K02T2R7CC-81134096-81133095
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 257890
Homologene: 114777
Slc35g3
Name: solute carrier family 35, member G3
Synonyms: Amac1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 56293
Homologene: 89413
Gm8122
Name: predicted gene 8122
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
Ier5l
Name: immediate early response 5-like
Synonyms: 2610524G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 72500
Homologene: 69468
Olfr1181
Name: olfactory receptor 1181
Synonyms: MOR225-9P, GA_x6K02T2Q125-49911636-49910701
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258060
Homologene: 86666
Gm6124
Name: predicted gene 6124
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 620018
Homologene: 45597
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 37,135,519 bp
  • A to G, chromosome 1 at 92,620,178 bp
  • A to T, chromosome 1 at 134,324,077 bp
  • A to G, chromosome 1 at 136,170,009 bp
  • A to G, chromosome 2 at 30,473,159 bp
  • A to T, chromosome 2 at 35,315,915 bp
  • T to C, chromosome 2 at 36,850,532 bp
  • T to A, chromosome 2 at 88,423,447 bp
  • T to A, chromosome 2 at 111,269,852 bp
  • G to A, chromosome 2 at 130,390,963 bp
  • T to C, chromosome 2 at 144,260,754 bp
  • T to C, chromosome 3 at 64,117,187 bp
  • G to A, chromosome 4 at 83,230,503 bp
  • A to G, chromosome 4 at 118,212,401 bp
  • T to C, chromosome 4 at 123,920,035 bp
  • A to G, chromosome 4 at 132,367,991 bp
  • A to G, chromosome 4 at 155,555,102 bp
  • G to A, chromosome 5 at 36,031,286 bp
  • T to C, chromosome 5 at 113,577,350 bp
  • A to G, chromosome 5 at 150,991,538 bp
  • C to T, chromosome 7 at 19,190,760 bp
  • A to G, chromosome 7 at 39,223,004 bp
  • T to C, chromosome 7 at 106,603,562 bp
  • T to A, chromosome 8 at 116,583,168 bp
  • G to A, chromosome 9 at 35,561,184 bp
  • T to G, chromosome 10 at 4,103,302 bp
  • A to G, chromosome 10 at 117,702,307 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to G, chromosome 11 at 23,655,705 bp
  • A to G, chromosome 11 at 69,760,454 bp
  • A to G, chromosome 11 at 78,023,544 bp
  • A to G, chromosome 11 at 100,625,027 bp
  • A to T, chromosome 11 at 116,539,670 bp
  • G to A, chromosome 12 at 76,623,114 bp
  • A to T, chromosome 12 at 118,127,106 bp
  • G to A, chromosome 13 at 54,724,308 bp
  • T to A, chromosome 13 at 84,286,790 bp
  • C to A, chromosome 14 at 29,990,881 bp
  • T to G, chromosome 14 at 43,234,020 bp
  • C to A, chromosome 14 at 50,710,360 bp
  • T to A, chromosome 14 at 79,863,330 bp
  • T to G, chromosome 15 at 65,881,539 bp
  • T to A, chromosome 15 at 79,130,478 bp
  • G to T, chromosome 16 at 35,964,097 bp
  • T to G, chromosome 16 at 44,435,442 bp
  • A to T, chromosome 17 at 33,631,213 bp
  • A to T, chromosome 17 at 34,906,032 bp
  • A to G, chromosome 17 at 67,815,224 bp
  • G to T, chromosome 19 at 9,016,747 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5727 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043190-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.