Strain Name:
C57BL/6J-MtgxR5850Btlr/Mmmh
Stock Number:
043226-MU
Citation ID:
RRID:MMRRC_043226-MU
Other Names:
R5850 (G1), C57BL/6J-MtgxR5850Btlr
Major Collection:

Gene Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, Acf7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 11426
Homologene: 136191
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 78757
VEGA: 15
Homologene: 34317
Kdsr
Name: 3-ketodihydrosphingosine reductase
Synonyms: Fvt1, 6330410P18Rik, 9430079B08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70750
HGNC: HGNC:4021
Homologene: 1539
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 77987
VEGA: 10
Homologene: 4973
Smyd3
Name: SET and MYND domain containing 3
Synonyms: Zmynd1, 2410008A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 69726
Homologene: 41491
Usp15
Name: ubiquitin specific peptidase 15
Synonyms: E430033I05Rik, 4921514G19Rik, Gcap18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 14479
VEGA: 10
Homologene: 101542
Zc3h14
Name: zinc finger CCCH type containing 14
Synonyms: 1700016A15Rik, 2700069A02Rik, 1010001P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 75553
VEGA: 12
Homologene: 32605
Atf7ip
Name: activating transcription factor 7 interacting protein
Synonyms: ATFa-associated Modulator, 2610204M12Rik, Mcaf1, AM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 54343
Homologene: 10051
Os9
Name: amplified in osteosarcoma
Synonyms: 4632413K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 216440
VEGA: 10
Homologene: 31409
Cnn3
Name: calponin 3, acidic
Synonyms: 1600014M03Rik, Calpo3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 71994
HGNC: HGNC:2157
Homologene: 37533
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: RPA194, 194kDa, Rpo1-4, 2900087K15Rik, 3010014K16Rik, mRPA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 20019
Homologene: 7033
Slc19a2
Name: solute carrier family 19 (thiamine transporter), member 2
Synonyms: THTR1, TRMA, DDA1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 116914
Homologene: 38258
Wdr45b
Name: WD repeat domain 45B
Synonyms: Wdr45l, D16Bwg0193e, 0610008N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 66840
Homologene: 6028
Ccdc141
Name: coiled-coil domain containing 141
Synonyms: 2610301F02Rik, CAMDI, ENSMUSG00000075261
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 545428
Homologene: 52149
Nmnat1
Name: nicotinamide nucleotide adenylyltransferase 1
Synonyms: 2610529L11Rik, D4Cole1e, nmnat, 5730441G13Rik, nicotinamide mononucleotide adenylyl transferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 66454
Homologene: 39074
Ganab
Name: alpha glucosidase 2 alpha neutral subunit
Synonyms: G2an, GluII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 14376
VEGA: 19
HGNC: HGNC:4138
Homologene: 5426
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: CC1, Min
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Ext2
Name: exostoses (multiple) 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 14043
HGNC: HGNC:3513
Homologene: 345
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225115
Homologene: 25090
Dnajc12
Name: DnaJ heat shock protein family (Hsp40) member C12
Synonyms: Jdp1, mJDP1, J domain protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 30045
Homologene: 8492
Ptgs2
Name: prostaglandin-endoperoxide synthase 2
Synonyms: PGHS-2, cyclooxygenase-2, Pghs2, PHS-2, COX2, Tis10, prostaglandin G/H synthase, cyclooxygenase 2, Cox-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 19225
HGNC: HGNC:9605
Homologene: 31000
Prf1
Name: perforin 1 (pore forming protein)
Synonyms: perforin, Pfp, Pfn, Prf-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 18646
VEGA: 10
HGNC: HGNC:9360
Homologene: 3698
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Hmgcll1
Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208982
Homologene: 27863
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: D12Ertd777e, Nesp2g, nesprin-2, syne-2, dice, Cpfl8, 6820443O06Rik, diminished cone electroretinogram
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 319565
Homologene: 56700
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 434341
Homologene: 88935
Abhd14a
Name: abhydrolase domain containing 14A
Synonyms: 1110013B16Rik, Dorz1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 68644
Homologene: 22889
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Fmnl1
Name: formin-like 1
Synonyms: formin-related gene in leukocytes, 8030453N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 57778
HGNC: HGNC:1212
Homologene: 135620
Apbb1
Name: amyloid beta (A4) precursor protein-binding, family B, member 1
Synonyms: Rir, Fe65
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11785
HGNC: HGNC:581
Homologene: 898
Aox4
Name: aldehyde oxidase 4
Synonyms: 2310003G12Rik, AOH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 71872
Homologene: 70273
4930553M12Rik
Name: RIKEN cDNA 4930553M12 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
Oxa1l
Name: oxidase assembly 1-like
Synonyms: 1810020M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 69089
HGNC: HGNC:8526
Homologene: 31281
Smco1
Name: single-pass membrane protein with coiled-coil domains 1
Synonyms: 2310010M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 69576
Homologene: 18842
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 639774
Homologene: 106613
Ecm1
Name: extracellular matrix protein 1
Synonyms: p85
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 13601
HGNC: HGNC:3153
Homologene: 3260
Ergic2
Name: ERGIC and golgi 2
Synonyms: 4930572C01Rik, 1200009B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 67456
Homologene: 6574
Dlgap1
Name: DLG associated protein 1
Synonyms: SAPAP1, Sapap1, D17Bwg0511e, Gkap, 4933422O14Rik, GKAP/SAPAP, DAP-1 beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224997
HGNC: HGNC:2905
Homologene: 31258
Apold1
Name: apolipoprotein L domain containing 1
Synonyms: LOC381823
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 381823
Homologene: 12781
Padi1
Name: peptidyl arginine deiminase, type I
Synonyms: Pdi1, Pad type 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 18599
Homologene: 7881
Bcl2l15
Name: BCLl2-like 15
Synonyms: LOC229672, Bfk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229672
Homologene: 19060
Tpm2
Name: tropomyosin 2, beta
Synonyms: Tpm-2, Trop-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 22004
Homologene: 134309
Ubap1l
Name: ubiquitin-associated protein 1-like
Synonyms: LOC208080, Gm514
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208080
VEGA: 9
Homologene: 19477
Zfp703
Name: zinc finger protein 703
Synonyms: Zeppo1, Csmn1, End2, 1110032O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 353310
Homologene: 81391
Nme9
Name: NME/NM23 family member 9
Synonyms: Txndc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 623534
Homologene: 52255
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,255,621 bp
  • A to T, chromosome 1 at 106,755,442 bp
  • A to G, chromosome 1 at 150,105,376 bp
  • A to G, chromosome 1 at 164,263,456 bp
  • G to A, chromosome 1 at 179,043,855 bp
  • T to A, chromosome 2 at 77,029,403 bp
  • A to T, chromosome 2 at 93,813,659 bp
  • T to C, chromosome 3 at 95,734,230 bp
  • T to A, chromosome 3 at 103,836,116 bp
  • T to C, chromosome 3 at 121,451,928 bp
  • T to C, chromosome 4 at 43,523,296 bp
  • T to C, chromosome 4 at 88,868,359 bp
  • C to A, chromosome 4 at 111,950,193 bp
  • T to C, chromosome 4 at 123,507,306 bp
  • A to G, chromosome 4 at 140,814,830 bp
  • G to A, chromosome 4 at 149,469,667 bp
  • T to A, chromosome 6 at 71,926,683 bp
  • T to C, chromosome 6 at 134,984,095 bp
  • T to C, chromosome 6 at 136,566,787 bp
  • C to A, chromosome 6 at 148,183,107 bp
  • GCCAGGGCC to GC, chromosome 7 at 80,098,344 bp
  • A to G, chromosome 7 at 105,567,583 bp
  • C to T, chromosome 8 at 26,979,205 bp
  • A to G, chromosome 8 at 94,521,047 bp
  • A to G, chromosome 9 at 65,373,763 bp
  • T to C, chromosome 9 at 76,074,757 bp
  • G to A, chromosome 9 at 99,469,679 bp
  • A to C, chromosome 9 at 106,440,349 bp
  • A to T, chromosome 9 at 108,114,950 bp
  • T to C, chromosome 10 at 50,710,953 bp
  • G to T, chromosome 10 at 61,300,193 bp
  • A to G, chromosome 10 at 63,407,187 bp
  • A to G, chromosome 10 at 123,124,512 bp
  • TTCCTCCTCCTCCTCCTCCTC to TTCCTCCTCCTCCTCCTC, chromosome 10 at 127,098,479 bp
  • A to G, chromosome 11 at 103,195,285 bp
  • C to A, chromosome 11 at 109,977,813 bp
  • A to G, chromosome 11 at 121,331,097 bp
  • A to G, chromosome 12 at 76,097,975 bp
  • A to G, chromosome 12 at 98,779,155 bp
  • T to G, chromosome 14 at 54,367,664 bp
  • G to A, chromosome 15 at 6,794,006 bp
  • A to T, chromosome 16 at 32,273,856 bp
  • G to A, chromosome 17 at 70,787,092 bp
  • T to A, chromosome 18 at 5,098,900 bp
  • T to C, chromosome 18 at 34,318,063 bp
  • C to T, chromosome 19 at 8,911,707 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5850 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043226-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.