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Strain Name:
C57BL/6J-MtgxR5618Btlr/Mmmh
Stock Number:
043277-MU
Citation ID:
RRID:MMRRC_043277-MU
Other Names:
R5618 (G1)
Major Collection:

Strain Information

Atg7
Name: autophagy related 7
Synonyms: 1810013K23Rik, Apg7l
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74244
Homologene: 4662
Sema7a
Name: sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A
Synonyms: Semaphorin K1, CDw108, Semal, 2900057C09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20361
VEGA: 9
Homologene: 2678
Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81897
Homologene: 68126
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Exog
Name: exo/endonuclease G
Synonyms: ENGL-a, ENDOGL2, ENGL-B, Endogl1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208194
VEGA: 9
HGNC: HGNC:3347
Homologene: 3745
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 26,634,875 bp
  • C to T, chromosome 2 at 58,462,943 bp
  • C to A, chromosome 2 at 120,369,306 bp
  • A to T, chromosome 2 at 155,437,897 bp
  • C to T, chromosome 3 at 32,591,207 bp
  • C to T, chromosome 3 at 145,004,977 bp
  • A to C, chromosome 4 at 58,070,537 bp
  • C to T, chromosome 4 at 143,133,537 bp
  • A to T, chromosome 4 at 143,850,693 bp
  • A to T, chromosome 4 at 149,269,889 bp
  • A to G, chromosome 5 at 21,742,788 bp
  • A to T, chromosome 5 at 43,729,907 bp
  • A to T, chromosome 5 at 86,306,295 bp
  • G to A, chromosome 5 at 107,548,199 bp
  • A to T, chromosome 5 at 123,361,202 bp
  • A to G, chromosome 6 at 84,106,824 bp
  • A to G, chromosome 6 at 114,673,699 bp
  • A to T, chromosome 6 at 118,126,316 bp
  • A to G, chromosome 7 at 28,178,986 bp
  • T to A, chromosome 7 at 44,741,732 bp
  • A to G, chromosome 7 at 140,162,272 bp
  • A to T, chromosome 8 at 9,972,021 bp
  • A to T, chromosome 8 at 19,114,798 bp
  • A to G, chromosome 8 at 83,242,592 bp
  • T to C, chromosome 8 at 105,702,914 bp
  • A to T, chromosome 9 at 36,843,932 bp
  • C to T, chromosome 9 at 38,498,740 bp
  • A to T, chromosome 9 at 39,590,704 bp
  • A to T, chromosome 9 at 57,960,283 bp
  • A to T, chromosome 9 at 106,224,739 bp
  • G to T, chromosome 9 at 119,462,751 bp
  • T to C, chromosome 10 at 79,148,921 bp
  • T to C, chromosome 10 at 80,804,576 bp
  • T to C, chromosome 10 at 127,010,577 bp
  • A to T, chromosome 10 at 130,479,948 bp
  • T to A, chromosome 11 at 75,410,184 bp
  • T to G, chromosome 11 at 78,154,240 bp
  • T to G, chromosome 11 at 78,304,080 bp
  • C to T, chromosome 11 at 86,663,562 bp
  • G to A, chromosome 13 at 59,726,400 bp
  • T to A, chromosome 13 at 76,173,426 bp
  • T to A, chromosome 14 at 34,691,906 bp
  • T to C, chromosome 14 at 57,481,508 bp
  • A to G, chromosome 15 at 31,598,015 bp
  • C to A, chromosome 15 at 88,974,566 bp
  • A to G, chromosome 16 at 15,809,612 bp
  • A to G, chromosome 16 at 32,754,253 bp
  • A to G, chromosome 16 at 52,152,668 bp
  • G to A, chromosome 17 at 25,184,989 bp
  • T to A, chromosome 17 at 35,379,925 bp
  • A to T, chromosome 17 at 71,455,727 bp
  • A to T, chromosome 18 at 47,281,948 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5618 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043277-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.