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Strain Name:
C57BL/6J-MtgxR5634Btlr/Mmmh
Stock Number:
043285-MU
Citation ID:
RRID:MMRRC_043285-MU
Other Names:
R5634 (G1)
Major Collection:

Strain Information

Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72568
Homologene: 35252
Eif2ak4
Name: eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms: GCN2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27103
Homologene: 40891
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F32S1, D15F32S1h, D7H15F37S1, rjs, jdf2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Pias1
Name: protein inhibitor of activated STAT 1
Synonyms: GBP, 2900068C24Rik, Ddxbp1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56469
VEGA: 9
HGNC: HGNC:2752
Homologene: 22953
Aen
Name: apoptosis enhancing nuclease
Synonyms: 2700083B06Rik, Isg20l1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68048
Homologene: 23382
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 40,539,376 bp
  • C to A, chromosome 1 at 63,062,283 bp
  • C to T, chromosome 1 at 91,444,707 bp
  • T to C, chromosome 1 at 104,975,075 bp
  • T to A, chromosome 1 at 180,669,198 bp
  • A to C, chromosome 2 at 25,364,133 bp
  • G to T, chromosome 2 at 31,333,881 bp
  • A to G, chromosome 2 at 106,403,980 bp
  • A to G, chromosome 2 at 111,307,092 bp
  • A to T, chromosome 2 at 118,462,311 bp
  • A to G, chromosome 2 at 119,973,095 bp
  • A to T, chromosome 2 at 127,759,650 bp
  • A to T, chromosome 2 at 129,039,486 bp
  • T to A, chromosome 4 at 49,792,843 bp
  • T to C, chromosome 4 at 76,072,018 bp
  • A to G, chromosome 5 at 72,529,490 bp
  • A to T, chromosome 5 at 104,815,289 bp
  • T to C, chromosome 5 at 118,560,850 bp
  • G to A, chromosome 6 at 32,237,723 bp
  • A to G, chromosome 6 at 71,509,617 bp
  • A to G, chromosome 7 at 45,734,082 bp
  • G to A, chromosome 7 at 56,206,783 bp
  • A to T, chromosome 7 at 78,902,507 bp
  • A to C, chromosome 7 at 131,371,343 bp
  • T to C, chromosome 8 at 46,041,391 bp
  • A to T, chromosome 9 at 25,603,553 bp
  • T to C, chromosome 9 at 53,583,621 bp
  • T to C, chromosome 9 at 62,895,973 bp
  • G to A, chromosome 9 at 65,134,546 bp
  • T to A, chromosome 9 at 109,676,803 bp
  • T to A, chromosome 9 at 111,055,987 bp
  • T to A, chromosome 10 at 7,814,778 bp
  • C to A, chromosome 10 at 92,904,263 bp
  • T to C, chromosome 11 at 6,602,387 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • T to C, chromosome 11 at 102,310,324 bp
  • T to C, chromosome 11 at 103,542,014 bp
  • T to A, chromosome 11 at 105,336,214 bp
  • T to C, chromosome 11 at 106,330,004 bp
  • T to A, chromosome 11 at 108,412,049 bp
  • T to C, chromosome 11 at 113,851,714 bp
  • T to C, chromosome 11 at 118,052,926 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • T to A, chromosome 12 at 86,952,975 bp
  • T to A, chromosome 12 at 101,043,521 bp
  • T to C, chromosome 14 at 96,240,271 bp
  • T to A, chromosome 15 at 55,518,298 bp
  • T to A, chromosome 15 at 89,378,207 bp
  • C to A, chromosome 15 at 101,528,649 bp
  • C to T, chromosome 17 at 64,292,867 bp
  • A to G, chromosome 17 at 87,342,087 bp
  • C to A, chromosome 18 at 69,636,847 bp
  • A to G, chromosome 19 at 4,840,665 bp
  • A to G, chromosome 19 at 6,226,860 bp
  • GCTTCTT to GCTT, chromosome 19 at 38,113,925 bp
  • G to A, chromosome 19 at 40,072,414 bp
  • A to G, chromosome 19 at 42,740,485 bp
  • T to C, chromosome 19 at 47,733,871 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5634 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043285-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.