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Strain Name:
C57BL/6J-MtgxR5639Btlr/Mmmh
Stock Number:
043288-MU
Citation ID:
RRID:MMRRC_043288-MU
Other Names:
R5639 (G1)
Major Collection:

Strain Information

Trmo
Name: tRNA methyltransferase O
Synonyms: 5830415F09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74753
Homologene: 32316
Ccn1
Name: cellular communication network factor 1
Synonyms: CCN1, Cyr61
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16007
HGNC: HGNC:2654
Homologene: 1194
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Drd5
Name: dopamine receptor D5
Synonyms: DRD1b, Drd-5, Gpcr1, Drd1b, D5R
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13492
HGNC: HGNC:3026
Homologene: 20216
Armc8
Name: armadillo repeat containing 8
Synonyms: HSPC056, 1200015K23Rik, Gid5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74125
Homologene: 9121
Epc2
Name: enhancer of polycomb homolog 2
Synonyms: D2Ertd694e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227867
Homologene: 32274
Tep1
Name: telomerase associated protein 1
Synonyms: Tp1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21745
VEGA: 14
Homologene: 5157
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 46,739,668 bp
  • T to A, chromosome 1 at 105,589,315 bp
  • C to T, chromosome 2 at 29,990,993 bp
  • A to G, chromosome 2 at 49,451,891 bp
  • T to C, chromosome 2 at 89,590,725 bp
  • T to C, chromosome 2 at 165,353,009 bp
  • C to T, chromosome 3 at 27,426,153 bp
  • G to T, chromosome 3 at 100,056,166 bp
  • A to C, chromosome 3 at 145,648,697 bp
  • T to C, chromosome 4 at 46,382,073 bp
  • T to A, chromosome 4 at 49,530,153 bp
  • C to A, chromosome 4 at 111,950,193 bp
  • A to G, chromosome 4 at 139,452,648 bp
  • A to T, chromosome 4 at 139,659,796 bp
  • T to A, chromosome 5 at 38,319,835 bp
  • G to A, chromosome 5 at 109,092,800 bp
  • T to A, chromosome 5 at 116,591,359 bp
  • T to C, chromosome 6 at 118,539,724 bp
  • C to A, chromosome 7 at 4,394,131 bp
  • A to G, chromosome 7 at 19,092,600 bp
  • T to C, chromosome 7 at 25,383,325 bp
  • G to A, chromosome 7 at 26,457,030 bp
  • T to C, chromosome 7 at 34,353,510 bp
  • T to A, chromosome 7 at 45,702,621 bp
  • A to G, chromosome 7 at 102,803,993 bp
  • A to C, chromosome 7 at 120,790,143 bp
  • T to G, chromosome 8 at 71,889,991 bp
  • T to C, chromosome 8 at 83,541,344 bp
  • T to C, chromosome 8 at 111,043,234 bp
  • A to T, chromosome 9 at 31,054,052 bp
  • T to C, chromosome 9 at 37,546,048 bp
  • A to T, chromosome 9 at 57,611,866 bp
  • T to A, chromosome 9 at 65,329,830 bp
  • A to T, chromosome 9 at 92,205,511 bp
  • A to G, chromosome 9 at 99,496,149 bp
  • T to C, chromosome 9 at 123,100,345 bp
  • A to G, chromosome 10 at 41,811,271 bp
  • A to T, chromosome 10 at 70,940,520 bp
  • A to G, chromosome 10 at 127,593,839 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • A to G, chromosome 12 at 17,278,593 bp
  • A to T, chromosome 12 at 85,747,476 bp
  • A to C, chromosome 12 at 103,171,572 bp
  • T to C, chromosome 13 at 77,024,783 bp
  • A to G, chromosome 14 at 50,853,605 bp
  • T to C, chromosome 14 at 115,092,747 bp
  • T to A, chromosome 14 at 121,275,382 bp
  • T to A, chromosome 15 at 65,997,750 bp
  • T to G, chromosome 15 at 84,214,128 bp
  • T to A, chromosome 15 at 85,882,698 bp
  • C to G, chromosome 15 at 86,030,767 bp
  • T to C, chromosome 15 at 99,947,050 bp
  • T to G, chromosome 15 at 101,756,760 bp
  • T to C, chromosome 18 at 32,265,714 bp
  • T to C, chromosome 19 at 5,088,750 bp
  • C to A, chromosome 19 at 20,623,422 bp
  • A to G, chromosome 19 at 45,776,999 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5639 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043288-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.