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Strain Name:
C57BL/6J-MtgxR5652Btlr/Mmmh
Stock Number:
043298-MU
Citation ID:
RRID:MMRRC_043298-MU
Other Names:
R5652 (G1)
Major Collection:

Strain Information

Pip5k1a
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
Synonyms: Pipk5a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18720
HGNC: HGNC:8994
Homologene: 93492
Sema7a
Name: sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A
Synonyms: Semaphorin K1, CDw108, Semal, 2900057C09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20361
VEGA: 9
Homologene: 2678
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20230
Homologene: 2232
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Cmklr2
Name: chemerin chemokine-like receptor 2
Synonyms: Gpr1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241070
HGNC: HGNC:4463
Homologene: 21094
Dgkh
Name: diacylglycerol kinase, eta
Synonyms: 5930402B05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 380921
VEGA: 14
HGNC: HGNC:2854
Homologene: 99373
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 36,216,153 bp
  • A to T, chromosome 1 at 58,095,197 bp
  • A to G, chromosome 1 at 58,466,072 bp
  • A to G, chromosome 1 at 63,183,467 bp
  • T to A, chromosome 1 at 135,882,597 bp
  • A to C, chromosome 1 at 166,165,145 bp
  • A to G, chromosome 1 at 189,657,082 bp
  • A to G, chromosome 2 at 31,185,589 bp
  • A to G, chromosome 2 at 37,801,126 bp
  • A to T, chromosome 2 at 76,881,753 bp
  • T to A, chromosome 2 at 85,331,373 bp
  • T to C, chromosome 2 at 151,589,215 bp
  • T to A, chromosome 2 at 165,807,698 bp
  • T to C, chromosome 2 at 178,358,705 bp
  • C to T, chromosome 3 at 39,002,968 bp
  • T to C, chromosome 3 at 95,067,439 bp
  • T to C, chromosome 3 at 108,396,735 bp
  • T to A, chromosome 4 at 94,855,324 bp
  • T to C, chromosome 4 at 130,764,127 bp
  • A to G, chromosome 5 at 73,418,141 bp
  • G to A, chromosome 5 at 104,059,780 bp
  • A to T, chromosome 5 at 109,429,564 bp
  • A to T, chromosome 5 at 127,784,795 bp
  • A to T, chromosome 5 at 145,349,325 bp
  • T to C, chromosome 6 at 29,135,409 bp
  • A to G, chromosome 6 at 121,840,181 bp
  • G to T, chromosome 6 at 146,773,328 bp
  • C to T, chromosome 7 at 30,959,031 bp
  • A to T, chromosome 7 at 65,312,443 bp
  • A to G, chromosome 7 at 75,866,147 bp
  • C to T, chromosome 7 at 130,261,863 bp
  • G to A, chromosome 7 at 138,280,046 bp
  • C to A, chromosome 8 at 60,919,353 bp
  • A to G, chromosome 8 at 83,929,815 bp
  • A to T, chromosome 9 at 7,116,638 bp
  • A to G, chromosome 9 at 18,815,626 bp
  • G to A, chromosome 9 at 20,437,841 bp
  • A to G, chromosome 9 at 21,090,312 bp
  • A to G, chromosome 9 at 22,424,490 bp
  • T to C, chromosome 9 at 38,180,815 bp
  • T to A, chromosome 9 at 50,768,405 bp
  • A to G, chromosome 9 at 57,960,659 bp
  • G to A, chromosome 9 at 108,838,472 bp
  • T to A, chromosome 10 at 63,135,801 bp
  • A to T, chromosome 10 at 93,555,586 bp
  • C to A, chromosome 10 at 111,977,383 bp
  • A to T, chromosome 10 at 126,899,625 bp
  • T to C, chromosome 11 at 8,909,889 bp
  • A to G, chromosome 11 at 32,422,812 bp
  • A to G, chromosome 11 at 53,537,217 bp
  • A to G, chromosome 11 at 58,206,629 bp
  • G to T, chromosome 11 at 65,024,238 bp
  • T to A, chromosome 11 at 91,575,830 bp
  • G to A, chromosome 11 at 104,338,166 bp
  • C to T, chromosome 11 at 106,008,985 bp
  • G to A, chromosome 12 at 65,016,650 bp
  • G to A, chromosome 12 at 110,665,988 bp
  • A to G, chromosome 13 at 17,991,706 bp
  • A to G, chromosome 13 at 23,696,236 bp
  • T to A, chromosome 13 at 46,674,338 bp
  • A to T, chromosome 13 at 65,182,989 bp
  • A to T, chromosome 14 at 20,713,427 bp
  • T to C, chromosome 14 at 51,418,572 bp
  • T to A, chromosome 14 at 54,366,832 bp
  • G to C, chromosome 14 at 55,773,443 bp
  • T to C, chromosome 14 at 78,627,761 bp
  • T to C, chromosome 14 at 88,467,324 bp
  • T to C, chromosome 14 at 118,618,927 bp
  • T to A, chromosome 15 at 57,951,666 bp
  • A to G, chromosome 15 at 97,758,437 bp
  • A to G, chromosome 15 at 99,082,264 bp
  • T to G, chromosome 15 at 99,945,372 bp
  • T to A, chromosome 15 at 102,216,203 bp
  • A to T, chromosome 16 at 17,655,191 bp
  • A to G, chromosome 16 at 19,065,670 bp
  • C to A, chromosome 16 at 20,120,247 bp
  • A to G, chromosome 16 at 58,872,484 bp
  • C to T, chromosome 16 at 85,899,268 bp
  • A to G, chromosome 17 at 20,540,519 bp
  • C to T, chromosome 17 at 35,686,508 bp
  • T to A, chromosome 17 at 51,742,795 bp
  • G to A, chromosome 18 at 36,968,836 bp
  • G to C, chromosome 18 at 64,531,382 bp
  • A to G, chromosome 18 at 80,130,850 bp
  • T to C, chromosome 19 at 4,858,477 bp
  • T to C, chromosome 19 at 36,604,320 bp
  • T to A, chromosome 19 at 45,007,293 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5652 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043298-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.