Strain Name:
C57BL/6J-MtgxR5666Btlr
Stock Number:
043309-MU
Citation ID:
RRID:MMRRC_043309-MU
Other Names:
R5666 (G1)
Major Collection:

Gene Information

Gene Symbol: Pde1c [MGI:108413] (Mus musculus (mouse))
Name: phosphodiesterase 1C
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Sstr2 [MGI:98328] (Mus musculus (mouse))
Name: somatostatin receptor 2
Synonyms: sst2, Smstr2, SSTR-2, Smstr-2
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Foxm1 [MGI:1347487] (Mus musculus (mouse))
Name: forkhead box M1
Synonyms: WIN, Fkh16, HFH-11B, Foxm1b, Mpm2, Trident
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Abce1 [MGI:1195458] (Mus musculus (mouse))
Name: ATP-binding cassette, sub-family E (OABP), member 1
Synonyms: RNS4l (Eye), Oabp, Rnaseli
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Slc4a2 [MGI:109351] (Mus musculus (mouse))
Name: solute carrier family 4 (anion exchanger), member 2
Synonyms: Ae2, B3RP
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Fzd6 [MGI:108474] (Mus musculus (mouse))
Name: frizzled class receptor 6
Synonyms: Fz6
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Nmt1 [MGI:102579] (Mus musculus (mouse))
Name: N-myristoyltransferase 1
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 69,577,900 bp
  • A to G, chromosome 1 at 82,485,579 bp
  • T to C, chromosome 1 at 91,391,141 bp
  • A to G, chromosome 1 at 144,536,942 bp
  • C to A, chromosome 1 at 154,204,217 bp
  • C to T, chromosome 1 at 175,921,613 bp
  • T to A, chromosome 2 at 36,870,389 bp
  • T to C, chromosome 2 at 75,677,118 bp
  • T to C, chromosome 2 at 90,265,964 bp
  • C to T, chromosome 2 at 91,212,581 bp
  • C to G, chromosome 2 at 120,085,567 bp
  • T to A, chromosome 2 at 154,569,181 bp
  • A to G, chromosome 2 at 160,897,330 bp
  • A to G, chromosome 2 at 180,943,202 bp
  • T to C, chromosome 3 at 116,924,101 bp
  • A to T, chromosome 3 at 144,569,793 bp
  • A to T, chromosome 4 at 24,898,636 bp
  • C to T, chromosome 4 at 96,658,693 bp
  • T to C, chromosome 4 at 107,389,526 bp
  • T to A, chromosome 4 at 138,780,280 bp
  • G to T, chromosome 4 at 140,949,231 bp
  • A to G, chromosome 4 at 155,774,465 bp
  • C to T, chromosome 5 at 5,673,681 bp
  • G to T, chromosome 5 at 24,434,838 bp
  • T to C, chromosome 5 at 75,173,495 bp
  • T to C, chromosome 5 at 99,979,209 bp
  • A to T, chromosome 5 at 108,999,044 bp
  • T to C, chromosome 6 at 37,965,601 bp
  • A to C, chromosome 6 at 56,126,857 bp
  • A to T, chromosome 6 at 87,053,813 bp
  • C to T, chromosome 6 at 128,373,167 bp
  • T to C, chromosome 6 at 128,375,555 bp
  • T to C, chromosome 6 at 131,630,379 bp
  • A to T, chromosome 7 at 73,441,717 bp
  • C to T, chromosome 7 at 132,054,896 bp
  • C to T, chromosome 7 at 143,807,431 bp
  • C to A, chromosome 8 at 79,690,277 bp
  • T to A, chromosome 9 at 23,473,463 bp
  • T to C, chromosome 9 at 44,715,781 bp
  • A to G, chromosome 9 at 45,344,955 bp
  • A to G, chromosome 9 at 62,060,511 bp
  • A to C, chromosome 11 at 52,144,698 bp
  • T to A, chromosome 11 at 67,221,352 bp
  • T to C, chromosome 11 at 69,353,351 bp
  • G to T, chromosome 11 at 100,423,526 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • C to T, chromosome 11 at 103,058,215 bp
  • T to A, chromosome 11 at 113,624,713 bp
  • T to A, chromosome 12 at 51,999,860 bp
  • G to A, chromosome 12 at 75,950,959 bp
  • G to A, chromosome 12 at 81,378,031 bp
  • T to A, chromosome 13 at 46,531,083 bp
  • T to C, chromosome 13 at 93,045,949 bp
  • A to T, chromosome 15 at 39,031,115 bp
  • T to C, chromosome 15 at 76,454,233 bp
  • A to G, chromosome 16 at 17,575,951 bp
  • A to G, chromosome 16 at 21,222,491 bp
  • A to T, chromosome 16 at 45,250,419 bp
  • G to A, chromosome 16 at 58,797,061 bp
  • G to A, chromosome 16 at 96,718,164 bp
  • T to C, chromosome 18 at 34,954,247 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5666 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043309-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.