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Strain Name:
C57BL/6J-MtgxR5712Btlr/Mmmh
Stock Number:
043334-MU
Citation ID:
RRID:MMRRC_043334-MU
Other Names:
R5712 (G1)
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Maco1
Name: macoilin 1
Synonyms: 9230118A01Rik, C61, 1110007C24Rik, Tmem57
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66146
Homologene: 14449
Wnt7a
Name: wingless-type MMTV integration site family, member 7A
Synonyms: Wnt-7a, tw
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22421
Homologene: 20969
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2700025J07Rik, 2810454G14Rik, 2610102K23Rik, 5730534O06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72567
Homologene: 8832
Pcnt
Name: pericentrin (kendrin)
Synonyms: Pcnt2, m275Asp, m239Asp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18541
VEGA: 10
Lrch4
Name: leucine-rich repeats and calponin homology (CH) domain containing 4
Synonyms: 2900069C24Rik, LRRN4, LRN, 2810008P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231798
HGNC: HGNC:6691
Homologene: 20532
Gpatch2l
Name: G patch domain containing 2 like
Synonyms: 1700020O03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70373
VEGA: 12
Homologene: 9942
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 52,196,752 bp
  • A to C, chromosome 1 at 133,730,540 bp
  • A to G, chromosome 1 at 170,166,845 bp
  • T to C, chromosome 2 at 83,008,848 bp
  • T to C, chromosome 2 at 113,845,301 bp
  • T to A, chromosome 2 at 157,030,921 bp
  • C to T, chromosome 3 at 83,033,133 bp
  • T to A, chromosome 3 at 89,051,990 bp
  • T to A, chromosome 3 at 123,121,210 bp
  • A to G, chromosome 4 at 115,855,006 bp
  • G to T, chromosome 4 at 118,614,795 bp
  • A to G, chromosome 4 at 128,745,095 bp
  • G to T, chromosome 4 at 129,556,310 bp
  • A to G, chromosome 4 at 133,210,408 bp
  • A to T, chromosome 4 at 134,828,058 bp
  • A to C, chromosome 4 at 145,087,173 bp
  • T to A, chromosome 5 at 105,094,555 bp
  • T to C, chromosome 5 at 136,694,238 bp
  • A to G, chromosome 5 at 137,400,098 bp
  • A to C, chromosome 5 at 137,637,926 bp
  • A to G, chromosome 5 at 145,044,753 bp
  • T to A, chromosome 6 at 58,956,699 bp
  • T to C, chromosome 6 at 91,366,204 bp
  • G to A, chromosome 7 at 4,784,289 bp
  • T to C, chromosome 7 at 12,998,083 bp
  • A to T, chromosome 7 at 23,835,500 bp
  • G to A, chromosome 7 at 28,737,472 bp
  • T to A, chromosome 7 at 105,619,536 bp
  • T to A, chromosome 8 at 25,919,650 bp
  • A to G, chromosome 8 at 60,937,298 bp
  • C to T, chromosome 9 at 31,414,636 bp
  • T to C, chromosome 9 at 108,082,908 bp
  • T to G, chromosome 10 at 20,333,531 bp
  • T to C, chromosome 10 at 76,429,271 bp
  • T to A, chromosome 10 at 127,856,887 bp
  • T to C, chromosome 11 at 4,218,378 bp
  • A to G, chromosome 11 at 70,453,232 bp
  • T to C, chromosome 11 at 76,907,032 bp
  • A to T, chromosome 11 at 87,874,853 bp
  • A to T, chromosome 11 at 120,620,851 bp
  • T to C, chromosome 12 at 70,042,769 bp
  • A to G, chromosome 12 at 86,244,480 bp
  • A to G, chromosome 12 at 87,429,113 bp
  • T to A, chromosome 12 at 111,424,042 bp
  • A to T, chromosome 13 at 49,563,519 bp
  • A to G, chromosome 14 at 30,074,997 bp
  • G to A, chromosome 14 at 56,471,399 bp
  • T to A, chromosome 14 at 75,658,900 bp
  • T to C, chromosome 14 at 79,428,765 bp
  • C to T, chromosome 15 at 64,754,866 bp
  • A to T, chromosome 15 at 91,702,222 bp
  • C to A, chromosome 17 at 43,626,408 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5712 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043334-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.