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Strain Name:
C57BL/6J-MtgxR5752Btlr/Mmmh
Stock Number:
043357-MU
Citation ID:
RRID:MMRRC_043357-MU
Other Names:
R5752 (G1)
Major Collection:

Strain Information

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Sh3gl3
Name: SH3-domain GRB2-like 3
Synonyms: SH3P13, EEN-B2, endophilin III, Sh3d2c, Sh3d2c2, endophilin A3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20408
Homologene: 37723
Ube2k
Name: ubiquitin-conjugating enzyme E2K
Synonyms: D5Ertd601e, Hip2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53323
HGNC: HGNC:4914
Homologene: 3903
Daam1
Name: dishevelled associated activator of morphogenesis 1
Synonyms: 1700066F09Rik, 2310028E21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 208846
VEGA: 12
Homologene: 36635
Pot1b
Name: protection of telomeres 1B
Synonyms: 2810458H16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72836
VEGA: 17
Homologene: 87058
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,547,972 bp
  • G to C, chromosome 1 at 85,577,202 bp
  • A to T, chromosome 1 at 164,137,242 bp
  • T to A, chromosome 1 at 191,575,893 bp
  • G to A, chromosome 2 at 25,399,769 bp
  • A to G, chromosome 2 at 41,295,612 bp
  • A to G, chromosome 2 at 51,627,868 bp
  • G to A, chromosome 2 at 67,184,635 bp
  • A to T, chromosome 2 at 76,947,984 bp
  • G to T, chromosome 2 at 118,711,051 bp
  • G to T, chromosome 2 at 130,906,544 bp
  • T to A, chromosome 3 at 121,732,404 bp
  • C to A, chromosome 3 at 152,452,122 bp
  • GGTG to GGTGTG, chromosome 4 at 129,223,980 bp
  • T to C, chromosome 4 at 145,148,970 bp
  • C to T, chromosome 4 at 155,996,304 bp
  • T to C, chromosome 5 at 5,589,204 bp
  • A to G, chromosome 5 at 65,566,068 bp
  • C to A, chromosome 5 at 92,323,856 bp
  • T to C, chromosome 5 at 107,139,807 bp
  • C to T, chromosome 6 at 7,689,365 bp
  • T to G, chromosome 6 at 8,015,025 bp
  • C to T, chromosome 6 at 83,831,270 bp
  • C to T, chromosome 7 at 25,355,114 bp
  • A to G, chromosome 7 at 78,783,595 bp
  • A to C, chromosome 7 at 81,899,703 bp
  • A to G, chromosome 7 at 82,174,948 bp
  • T to C, chromosome 7 at 99,767,948 bp
  • C to T, chromosome 7 at 127,109,244 bp
  • T to C, chromosome 8 at 27,156,586 bp
  • A to G, chromosome 8 at 70,165,617 bp
  • T to A, chromosome 8 at 83,397,041 bp
  • A to C, chromosome 9 at 8,120,745 bp
  • T to A, chromosome 9 at 61,407,471 bp
  • A to C, chromosome 9 at 104,192,774 bp
  • C to A, chromosome 10 at 83,520,380 bp
  • C to T, chromosome 10 at 86,758,346 bp
  • T to A, chromosome 10 at 128,041,928 bp
  • A to T, chromosome 11 at 55,289,237 bp
  • A to G, chromosome 12 at 44,282,887 bp
  • T to C, chromosome 12 at 71,946,546 bp
  • G to A, chromosome 12 at 102,313,119 bp
  • A to G, chromosome 12 at 119,262,614 bp
  • T to C, chromosome 13 at 59,743,202 bp
  • T to C, chromosome 13 at 89,679,950 bp
  • T to C, chromosome 15 at 88,932,728 bp
  • A to T, chromosome 15 at 100,704,126 bp
  • A to T, chromosome 17 at 32,631,332 bp
  • A to G, chromosome 17 at 33,818,063 bp
  • C to A, chromosome 17 at 46,890,040 bp
  • A to T, chromosome 17 at 55,687,834 bp
  • G to A, chromosome 18 at 39,286,672 bp
  • C to A, chromosome 19 at 4,626,020 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5752 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043357-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.