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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR5769Btlr/Mmmh
Stock Number:
043369-MU
Citation ID:
RRID:MMRRC_043369-MU
Other Names:
R5769 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250

Additional Resources
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Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
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ClinGen
Nup188
Name: nucleoporin 188
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227699
Homologene: 45683

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tesk2
Name: testis-specific kinase 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230661
Homologene: 5188

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Jrk
Name: jerky
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16469
HGNC: HGNC:6199
Homologene: 2762

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Fbxo38
Name: F-box protein 38
Synonyms: SP329, 6030410I24Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107035
VEGA: 18
Homologene: 34526

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Mms19
Name: MMS19 cytosolic iron-sulfur assembly component
Synonyms: 2610042O15Rik, Mms19, C86341, Mms19l
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72199
Homologene: 41480

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 37,950,750 bp
  • G to T, chromosome 1 at 91,412,949 bp
  • A to G, chromosome 1 at 135,452,257 bp
  • A to G, chromosome 1 at 180,179,116 bp
  • T to C, chromosome 1 at 191,372,666 bp
  • A to G, chromosome 2 at 30,330,735 bp
  • G to A, chromosome 2 at 104,434,953 bp
  • A to G, chromosome 2 at 150,268,278 bp
  • A to T, chromosome 2 at 150,925,998 bp
  • T to A, chromosome 3 at 72,928,235 bp
  • A to G, chromosome 3 at 145,135,731 bp
  • G to T, chromosome 4 at 105,013,321 bp
  • T to A, chromosome 4 at 105,015,644 bp
  • A to G, chromosome 4 at 116,802,315 bp
  • A to C, chromosome 4 at 117,728,965 bp
  • T to A, chromosome 4 at 138,898,110 bp
  • A to T, chromosome 4 at 155,902,400 bp
  • A to C, chromosome 5 at 8,683,426 bp
  • G to T, chromosome 5 at 30,958,291 bp
  • G to A, chromosome 5 at 32,317,522 bp
  • T to C, chromosome 5 at 120,916,854 bp
  • T to A, chromosome 5 at 138,646,333 bp
  • A to G, chromosome 6 at 47,815,512 bp
  • T to A, chromosome 6 at 66,611,833 bp
  • A to G, chromosome 6 at 83,050,600 bp
  • A to C, chromosome 7 at 12,615,282 bp
  • T to A, chromosome 7 at 62,378,113 bp
  • G to A, chromosome 7 at 109,978,738 bp
  • G to A, chromosome 7 at 120,089,952 bp
  • T to A, chromosome 7 at 127,559,822 bp
  • T to A, chromosome 8 at 20,897,527 bp
  • A to T, chromosome 8 at 71,386,219 bp
  • A to G, chromosome 9 at 78,064,967 bp
  • G to A, chromosome 9 at 99,093,159 bp
  • A to T, chromosome 9 at 121,448,838 bp
  • T to C, chromosome 10 at 20,960,082 bp
  • C to T, chromosome 10 at 75,638,019 bp
  • T to C, chromosome 10 at 105,370,046 bp
  • A to T, chromosome 10 at 127,876,889 bp
  • A to T, chromosome 11 at 86,346,003 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • A to G, chromosome 11 at 116,246,669 bp
  • T to A, chromosome 12 at 98,790,619 bp
  • A to G, chromosome 13 at 76,759,808 bp
  • T to A, chromosome 13 at 112,953,717 bp
  • T to C, chromosome 15 at 34,484,558 bp
  • T to C, chromosome 15 at 74,706,068 bp
  • T to C, chromosome 15 at 89,123,616 bp
  • C to T, chromosome 16 at 9,761,526 bp
  • A to G, chromosome 16 at 90,938,253 bp
  • A to T, chromosome 17 at 9,667,843 bp
  • T to C, chromosome 17 at 34,032,847 bp
  • A to T, chromosome 17 at 66,118,116 bp
  • A to T, chromosome 18 at 23,651,554 bp
  • A to T, chromosome 18 at 58,105,199 bp
  • G to A, chromosome 18 at 62,514,965 bp
  • T to C, chromosome 19 at 4,451,044 bp
  • A to G, chromosome 19 at 41,964,386 bp
  • T to A, chromosome 19 at 47,102,637 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5769 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
043369-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.