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Strain Name:
C57BL/6J-MtgxR5778Btlr/Mmmh
Stock Number:
043376-MU
Citation ID:
RRID:MMRRC_043376-MU
Other Names:
R5778 (G1)
Major Collection:

Strain Information

Eno2
Name: enolase 2, gamma neuronal
Synonyms: Eno-2, D6Ertd375e, NSE
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13807
HGNC: HGNC:3353
Homologene: 74414
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Dusp16
Name: dual specificity phosphatase 16
Synonyms: MKP-7, MKP7, 3830417M17Rik, D6Ertd213e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70686
Homologene: 15604
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: Tweek, FSA, 4932438A13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229227
Homologene: 52105
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 105,591,722 bp
  • G to T, chromosome 1 at 152,552,421 bp
  • A to T, chromosome 1 at 157,161,290 bp
  • T to G, chromosome 1 at 157,470,787 bp
  • C to T, chromosome 1 at 170,963,388 bp
  • G to T, chromosome 2 at 59,699,347 bp
  • T to A, chromosome 2 at 88,149,617 bp
  • T to C, chromosome 2 at 155,406,768 bp
  • A to C, chromosome 2 at 181,855,198 bp
  • A to T, chromosome 3 at 36,958,714 bp
  • T to A, chromosome 3 at 104,772,189 bp
  • A to G, chromosome 3 at 158,170,743 bp
  • A to G, chromosome 4 at 43,660,683 bp
  • C to T, chromosome 4 at 140,037,521 bp
  • A to T, chromosome 5 at 44,007,047 bp
  • A to T, chromosome 5 at 73,072,778 bp
  • T to C, chromosome 5 at 110,719,584 bp
  • G to A, chromosome 5 at 143,202,387 bp
  • A to T, chromosome 5 at 143,719,576 bp
  • A to T, chromosome 6 at 124,766,298 bp
  • G to T, chromosome 6 at 134,718,314 bp
  • A to T, chromosome 6 at 135,244,350 bp
  • A to T, chromosome 6 at 148,869,322 bp
  • A to T, chromosome 7 at 9,976,347 bp
  • A to T, chromosome 7 at 17,051,984 bp
  • C to G, chromosome 7 at 28,994,221 bp
  • T to C, chromosome 7 at 79,836,391 bp
  • T to C, chromosome 8 at 25,659,818 bp
  • T to C, chromosome 8 at 71,692,938 bp
  • T to A, chromosome 8 at 72,693,117 bp
  • A to G, chromosome 8 at 94,479,526 bp
  • G to A, chromosome 8 at 122,856,587 bp
  • A to G, chromosome 8 at 123,892,125 bp
  • T to A, chromosome 9 at 7,350,106 bp
  • G to T, chromosome 9 at 19,921,041 bp
  • C to A, chromosome 10 at 57,801,001 bp
  • A to G, chromosome 10 at 79,866,503 bp
  • A to G, chromosome 10 at 80,520,173 bp
  • C to A, chromosome 10 at 118,294,863 bp
  • A to G, chromosome 10 at 126,899,582 bp
  • G to A, chromosome 11 at 101,525,301 bp
  • A to T, chromosome 11 at 102,064,443 bp
  • A to T, chromosome 11 at 121,714,738 bp
  • T to A, chromosome 12 at 8,015,074 bp
  • T to C, chromosome 12 at 85,819,480 bp
  • A to T, chromosome 12 at 114,612,431 bp
  • C to A, chromosome 12 at 114,723,877 bp
  • A to G, chromosome 13 at 22,102,382 bp
  • C to T, chromosome 13 at 27,661,000 bp
  • T to A, chromosome 13 at 55,306,979 bp
  • C to T, chromosome 13 at 58,183,317 bp
  • T to C, chromosome 14 at 79,770,757 bp
  • A to G, chromosome 15 at 27,856,164 bp
  • T to A, chromosome 15 at 71,479,032 bp
  • A to T, chromosome 15 at 86,032,955 bp
  • A to T, chromosome 15 at 89,387,320 bp
  • T to C, chromosome 15 at 102,003,939 bp
  • T to A, chromosome 17 at 80,127,717 bp
  • A to T, chromosome 18 at 20,409,222 bp
  • T to A, chromosome 18 at 84,015,680 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5778 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043376-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.