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Strain Name:
C57BL/6J-MtgxR5806Btlr/Mmmh
Stock Number:
043392-MU
Citation ID:
RRID:MMRRC_043392-MU
Other Names:
R5806 (G1)
Major Collection:

Strain Information

Zfp143
Name: zinc finger protein 143
Synonyms: D7Ertd805e, pHZ-1, KRAB14, Zfp79, Zfp80-rs1, Staf
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20841
Homologene: 56444
Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117599
Homologene: 50463
Srrt
Name: serrate RNA effector molecule homolog (Arabidopsis)
Synonyms: Asr2, Ars2, 2810019G02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 83701
Homologene: 9298
Ddx55
Name: DEAD box helicase 55
Synonyms: 2810021H22Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 55
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67848
Homologene: 12196
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 133,355,511 bp
  • A to C, chromosome 1 at 140,509,496 bp
  • A to T, chromosome 1 at 171,531,152 bp
  • T to C, chromosome 2 at 88,683,151 bp
  • C to A, chromosome 2 at 130,397,473 bp
  • T to A, chromosome 2 at 143,963,331 bp
  • T to C, chromosome 2 at 181,199,730 bp
  • C to A, chromosome 2 at 181,502,887 bp
  • T to A, chromosome 3 at 68,950,476 bp
  • T to C, chromosome 3 at 109,080,578 bp
  • A to T, chromosome 3 at 154,827,611 bp
  • A to T, chromosome 4 at 119,468,466 bp
  • A to G, chromosome 4 at 123,371,887 bp
  • A to G, chromosome 4 at 126,586,106 bp
  • A to G, chromosome 4 at 127,355,925 bp
  • C to A, chromosome 5 at 20,651,204 bp
  • A to C, chromosome 5 at 21,823,563 bp
  • T to C, chromosome 5 at 31,040,770 bp
  • G to A, chromosome 5 at 93,167,578 bp
  • A to G, chromosome 5 at 99,240,872 bp
  • A to T, chromosome 5 at 110,755,554 bp
  • A to G, chromosome 5 at 124,559,199 bp
  • A to G, chromosome 5 at 130,877,819 bp
  • A to T, chromosome 5 at 137,297,917 bp
  • A to G, chromosome 6 at 115,736,412 bp
  • A to T, chromosome 6 at 125,181,154 bp
  • T to A, chromosome 7 at 27,029,079 bp
  • T to A, chromosome 7 at 27,250,625 bp
  • A to T, chromosome 7 at 44,115,877 bp
  • T to C, chromosome 7 at 79,448,848 bp
  • T to C, chromosome 7 at 106,158,206 bp
  • A to T, chromosome 7 at 110,086,235 bp
  • A to T, chromosome 7 at 127,559,163 bp
  • T to C, chromosome 7 at 135,704,605 bp
  • A to G, chromosome 7 at 141,862,835 bp
  • T to C, chromosome 7 at 144,461,268 bp
  • A to T, chromosome 8 at 105,378,910 bp
  • C to A, chromosome 8 at 105,837,496 bp
  • T to A, chromosome 8 at 123,381,504 bp
  • C to T, chromosome 9 at 50,618,450 bp
  • T to C, chromosome 9 at 72,962,054 bp
  • A to G, chromosome 10 at 24,907,221 bp
  • A to G, chromosome 10 at 93,490,984 bp
  • A to T, chromosome 10 at 118,166,658 bp
  • G to A, chromosome 10 at 120,092,519 bp
  • A to G, chromosome 11 at 60,776,920 bp
  • G to T, chromosome 11 at 60,780,382 bp
  • T to C, chromosome 11 at 67,181,315 bp
  • T to C, chromosome 11 at 68,892,963 bp
  • A to T, chromosome 11 at 73,887,547 bp
  • G to C, chromosome 11 at 106,398,705 bp
  • T to A, chromosome 12 at 32,204,953 bp
  • T to C, chromosome 12 at 70,890,020 bp
  • A to G, chromosome 12 at 103,403,397 bp
  • A to T, chromosome 12 at 110,651,653 bp
  • A to G, chromosome 13 at 55,663,337 bp
  • A to G, chromosome 13 at 93,093,937 bp
  • T to C, chromosome 13 at 95,042,040 bp
  • T to C, chromosome 13 at 98,268,497 bp
  • C to T, chromosome 13 at 100,444,735 bp
  • A to G, chromosome 14 at 31,667,512 bp
  • A to G, chromosome 14 at 54,261,690 bp
  • A to T, chromosome 14 at 54,282,807 bp
  • A to T, chromosome 15 at 10,319,204 bp
  • A to G, chromosome 15 at 101,950,502 bp
  • G to A, chromosome 16 at 57,161,693 bp
  • A to T, chromosome 16 at 97,454,151 bp
  • G to A, chromosome 17 at 37,481,726 bp
  • A to T, chromosome 17 at 56,710,717 bp
  • T to A, chromosome 18 at 66,076,355 bp
  • A to G, chromosome 18 at 78,856,482 bp
  • C to T, chromosome 18 at 84,558,614 bp
  • T to G, chromosome 19 at 36,918,549 bp
  • T to C, chromosome 19 at 45,026,367 bp
  • T to C, chromosome 19 at 46,366,410 bp
  • G to T, chromosome Y at 1,170,921 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5806 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043392-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.