Strain Name:
C.Cg-Tg(H2-Ea-HA)HACIIAjca/Mmjax
Stock Number:
043832-JAX
Citation ID:
RRID:MMRRC_043832-JAX
Other Names:
HACII, Tg(H2-Ea-HA)#Ajca

Gene Information

Tg(H2-Ea-HA)HACIIAjca
Name: transgene insertion HACII, Andrew J Caton
Synonyms: HACII
Type: Allele
Species: Influenza virus
Chromosome: random insertion
Alteration at locus: Transgenic
HA
Name: haemagglutinin, Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Type: Gene
Species: Influenza virus
Alteration at locus: Transgenic
H2-Ea
Name: histocompatibility 2, class II antigen E alpha
Synonyms: Ia3, H2-Ea-ps, Ia-3, I-Ealpha, H-2Ea
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Transgenic
Genetic Alterations
Random transgene insertion of influenza virus hemagglutinin (HA - NCBI Gene:956529) driven by the MHC Class II I-Ea pseudogene promoter (H2-Ea-ps - MGI:95900). 
Phenotype

Homozygous: Non-lethal. No abnormal physiology detected

Hetero/Hemizygous: No abnormal physiology detected. Induces CD4+ T cell tolerance to encoded epitopes from HA. When mated with TCR transgenic mice (strain Tg(Tcra/Tcrb)1Vbo or strain Tg(Tcra/Tcrb)#Ajca) the double transgenic mice spontaneously develop autoimmune inflammatory arthritis by distinct cellular pathways, depending on the specific TCR transgene strain.

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Autoantigens/genetics
  • CD4-Positive T-Lymphocytes/classification
  • CD4-Positive T-Lymphocytes/immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Phenotype
  • Receptors, Antigen, T-Cell/genetics
  • Receptors, Antigen, T-Cell/metabolism
  • Receptors, Interleukin-2/metabolism
  • Self Tolerance
  • Thymus Gland/cytology
  • Thymus Gland/immunology
  • Abatacept
  • Antigens, CD
  • Antigens, Differentiation/immunology
  • B-Lymphocytes/cytology
  • B-Lymphocytes/immunology
  • B7-1 Antigen/immunology
  • CD28 Antigens/immunology
  • CD40 Antigens/immunology
  • CD40 Ligand/immunology
  • CTLA-4 Antigen
  • Cell Differentiation
  • Clonal Anergy/immunology
  • DNA/immunology
  • Female
  • Immunoconjugates
  • Lymphocyte Activation/immunology
  • Male
  • Mice, Inbred MRL lpr
  • Models, Immunological
  • Receptors, CXCR5
  • Receptors, Chemokine
  • Receptors, Cytokine/biosynthesis
  • Receptors, Cytokine/immunology
  • Receptors, Interleukin-2/immunology
  • Spleen/cytology
  • Spleen/immunology
  • T-Lymphocytes, Helper-Inducer/immunology
  • Adoptive Transfer
  • Antigen Presentation/genetics
  • Antigen Presentation/immunology
  • Antigen-Presenting Cells/immunology
  • Antigen-Presenting Cells/metabolism
  • Antigen-Presenting Cells/virology
  • CD4-Positive T-Lymphocytes/transplantation
  • Cell Differentiation/genetics
  • Cell Differentiation/immunology
  • Cells, Cultured
  • Clone Cells
  • Heart Transplantation/immunology
  • Hemagglutinin Glycoproteins, Influenza Virus/biosynthesis
  • Hemagglutinin Glycoproteins, Influenza Virus/genetics
  • Histocompatibility Antigens Class II/immunology
  • Histocompatibility Antigens Class II/metabolism
  • Isoantigens/immunology
  • Isoantigens/metabolism
  • Lymphocyte Activation/genetics
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, SCID
  • Signal Transduction/genetics
  • Signal Transduction/immunology
  • Skin Transplantation/immunology
  • Antigen Presentation
  • Arthritis/immunology
  • Autoantigens/immunology
  • Autoimmune Diseases/immunology
  • Hemagglutinins/genetics
  • Hemagglutinins/immunology
  • Histocompatibility Antigens Class I/genetics
  • Peptides/immunology
  • Promoter Regions, Genetic
  • T-Lymphocytes, Regulatory/immunology
  • Antibodies, Neutralizing/immunology
  • Antibodies, Neutralizing/pharmacology
  • Arthritis/genetics
  • Arthritis/prevention & control
  • Autoimmunity/genetics
  • Autoimmunity/immunology
  • B-Lymphocytes/metabolism
  • CD4-Positive T-Lymphocytes/metabolism
  • Flow Cytometry
  • Forelimb/immunology
  • Forelimb/metabolism
  • Forelimb/pathology
  • Forkhead Transcription Factors/immunology
  • Forkhead Transcription Factors/metabolism
  • Hindlimb/immunology
  • Hindlimb/metabolism
  • Hindlimb/pathology
  • Receptors, Antigen, T-Cell/immunology
  • Sex Factors
  • Signal Transduction/drug effects
  • Tumor Necrosis Factor-alpha/immunology
  • Tumor Necrosis Factor-alpha/metabolism
Strain Development
Backcrossed 13 generations (N13) with BALB/cAnNCrl (Charles River Strain 028), then intermated (F10-15) to homozygosity. Maintained as homozygotes. Upon arrival at the MMRRC: backcrossing to BALB/cByJ (Jackson Strain #001026).
Suggested Control Mice
Wild-type littermates
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Andrew J. Caton, Ph.D., The Wistar Institute.
Primary Reference

Jordan MS, Boesteanu A, Reed AJ, Petrone AL, Holenbeck AE, Lerman MA, Naji A, Caton AJ. Thymic selection of CD4+CD25+ regulatory T cells induced by an agonist self-peptide. Nat Immunol. 2001 Apr;2(4):301-6. (Medline PMID: 11276200)

Seo SJ, Fields ML, Buckler JL, Reed AJ, Mandik-Nayak L, Nish SA, Noelle RJ, Turka LA, Finkelman FD, Caton AJ, Erikson J. The impact of T helper and T regulatory cells on the regulation of anti-double-stranded DNA B cells. Immunity. 2002 Apr;16(4):535-46. (Medline PMID: 11970877)

Reed AJ, Noorchashm H, Rostami SY, Zarrabi Y, Perate AR, Jeganathan AN, Caton AJ, Naji A. Alloreactive CD4 T cell activation in vivo: an autonomous function ofthe indirect pathway of alloantigen presentation. J Immunol. 2003 Dec15;171(12):6502-9. (Medline PMID: 14662850)

Rankin AL, Reed AJ, Oh S, Cozzo Picca C, Guay HM, Larkin J 3rd, Panarey L,Aitken MK, Koeberlein B, Lipsky PE, Tomaszewski JE, Naji A, Caton AJ. CD4+ Tcells recognizing a single self-peptide expressed by APCs induce spontaneousautoimmune arthritis. J Immunol. 2008 Jan 15;180(2):833-41. Erratum in: JImmunol. 2008 May 15;180(10):7047. (Medline PMID: 18178822)

Perng OA, Aitken M, Rankin AL, Garcia V, Kropf E, Erikson J, Garlick DS, CatonAJ. The degree of CD4+ T cell autoreactivity determines cellular pathwaysunderlying inflammatory arthritis. J Immunol. 2014 Apr 1;192(7):3043-56. doi:10.4049/jimmunol.1302528. Epub 2014 Mar 3. (Medline PMID: 24591372)

Colony and Husbandry Information

Related strains: HA28 (MMRRC:43825) and HA104 mice (MMRRC:43831)

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Albino
MMRRC Breeding System
Backcrossing then sib-mating
Generation
N13 (BALB/cAnNCrl, Charles River Strain 028), F10-15
Overall Breeding Performance
Poor
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3-4 weeks
Average Pups Weaned
Less than 4

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043832-JAX-HET-F
043832-JAX-HET-M
043832-JAX-WT-F
043832-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
043832-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.