Homozygous: Non-lethal. No abnormal physiology detected
Hetero/Hemizygous: No abnormal physiology detected. Induces CD4+ T cell tolerance to encoded epitopes from HA. When mated with TCR transgenic mice (strain Tg(Tcra/Tcrb)1Vbo or strain Tg(Tcra/Tcrb)#Ajca) the double transgenic mice spontaneously develop autoimmune inflammatory arthritis by distinct cellular pathways, depending on the specific TCR transgene strain.
Jordan MS, Boesteanu A, Reed AJ, Petrone AL, Holenbeck AE, Lerman MA, Naji A, Caton AJ. Thymic selection of CD4+CD25+ regulatory T cells induced by an agonist self-peptide. Nat Immunol. 2001 Apr;2(4):301-6. (Medline PMID: 11276200)
Seo SJ, Fields ML, Buckler JL, Reed AJ, Mandik-Nayak L, Nish SA, Noelle RJ, Turka LA, Finkelman FD, Caton AJ, Erikson J. The impact of T helper and T regulatory cells on the regulation of anti-double-stranded DNA B cells. Immunity. 2002 Apr;16(4):535-46. (Medline PMID: 11970877)
Reed AJ, Noorchashm H, Rostami SY, Zarrabi Y, Perate AR, Jeganathan AN, Caton AJ, Naji A. Alloreactive CD4 T cell activation in vivo: an autonomous function ofthe indirect pathway of alloantigen presentation. J Immunol. 2003 Dec15;171(12):6502-9. (Medline PMID: 14662850)
Rankin AL, Reed AJ, Oh S, Cozzo Picca C, Guay HM, Larkin J 3rd, Panarey L,Aitken MK, Koeberlein B, Lipsky PE, Tomaszewski JE, Naji A, Caton AJ. CD4+ Tcells recognizing a single self-peptide expressed by APCs induce spontaneousautoimmune arthritis. J Immunol. 2008 Jan 15;180(2):833-41. Erratum in: JImmunol. 2008 May 15;180(10):7047. (Medline PMID: 18178822)
Perng OA, Aitken M, Rankin AL, Garcia V, Kropf E, Erikson J, Garlick DS, CatonAJ. The degree of CD4+ T cell autoreactivity determines cellular pathwaysunderlying inflammatory arthritis. J Immunol. 2014 Apr 1;192(7):3043-56. doi:10.4049/jimmunol.1302528. Epub 2014 Mar 3. (Medline PMID: 24591372)
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Hetero/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||6 to 8 months||6 to 8 months|
Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at email@example.com for more details.
Cryopreserved material may be available upon request, please inquire to firstname.lastname@example.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|MMRRC Item #||Description||Distribution Fee / Unit (US $)||Units||Notes|
|Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
|$218.00 / Non-Profit||Per Mouse||The email@example.com may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).|
|Cryopreserved material may be available upon request, please inquire to firstname.lastname@example.org for more information.|
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.