Homozygous: Decreased TNF secretion from macrophages of mice stimulated with polyinosinic:polycytidylic acid ("poly(I:C)").
Hetero/Hemizygous: Not Characterized
The TALEN constructs recognizing these two targets were built using the TALEN toolbox (from Addgene, deposited by Feng Zhang MIT). TALEN mRNAs were in vitro transcribed using the Invitrogen mMESSAGE mMACHINE kit and used for microinjection. This strain has a 171-bp deletion, which encompasses the ATG start codon. The original mutant was generated on a C57BL/6J background; all subsequent crosses to maintain strain were on C57BL/6J background only.
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Undetermined||Undetermined|
When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.