Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5849Btlr/Mmmh
Stock Number:
044066-MU
Citation ID:
RRID:MMRRC_044066-MU
Other Names:
R5849 (G1)
Major Collection:

Strain Information

Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Rnf220
Name: ring finger protein 220
Synonyms: 5730503K05Rik, 4931406I20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66743
Homologene: 10036
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Madcam1
Name: mucosal vascular addressin cell adhesion molecule 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17123
VEGA: 10
HGNC: HGNC:6765
Homologene: 8413
Aopep
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Zfp384
Name: zinc finger protein 384
Synonyms: C130073D16Rik, Ciz, Nmp4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269800
Homologene: 15849
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,736,684 bp
  • T to A, chromosome 1 at 75,527,355 bp
  • T to A, chromosome 1 at 133,608,914 bp
  • G to A, chromosome 2 at 25,823,256 bp
  • A to T, chromosome 2 at 59,799,904 bp
  • T to C, chromosome 2 at 76,746,242 bp
  • A to T, chromosome 2 at 85,770,424 bp
  • A to T, chromosome 2 at 112,129,878 bp
  • T to C, chromosome 2 at 113,834,847 bp
  • T to A, chromosome 2 at 167,106,026 bp
  • T to A, chromosome 3 at 35,916,184 bp
  • A to T, chromosome 3 at 36,032,877 bp
  • A to G, chromosome 3 at 105,458,795 bp
  • T to G, chromosome 4 at 62,086,935 bp
  • T to C, chromosome 4 at 86,825,986 bp
  • T to C, chromosome 4 at 88,868,359 bp
  • T to C, chromosome 4 at 117,277,612 bp
  • C to T, chromosome 4 at 118,161,840 bp
  • T to A, chromosome 5 at 29,658,409 bp
  • C to G, chromosome 5 at 36,973,264 bp
  • T to G, chromosome 5 at 92,604,995 bp
  • T to C, chromosome 5 at 121,373,471 bp
  • T to A, chromosome 6 at 24,891,556 bp
  • A to G, chromosome 6 at 37,957,729 bp
  • T to A, chromosome 6 at 40,476,903 bp
  • G to A, chromosome 6 at 125,024,099 bp
  • G to T, chromosome 7 at 5,031,087 bp
  • A to T, chromosome 7 at 6,460,994 bp
  • A to G, chromosome 7 at 28,744,258 bp
  • A to G, chromosome 7 at 68,190,033 bp
  • T to C, chromosome 7 at 76,325,098 bp
  • A to T, chromosome 7 at 80,803,158 bp
  • T to A, chromosome 7 at 103,085,521 bp
  • A to G, chromosome 7 at 127,317,320 bp
  • T to C, chromosome 7 at 143,015,587 bp
  • G to A, chromosome 9 at 45,868,088 bp
  • G to A, chromosome 9 at 107,682,616 bp
  • A to G, chromosome 9 at 120,453,693 bp
  • A to G, chromosome 10 at 41,348,049 bp
  • T to A, chromosome 10 at 79,664,990 bp
  • C to T, chromosome 10 at 81,499,323 bp
  • T to A, chromosome 11 at 44,990,504 bp
  • T to A, chromosome 11 at 59,804,760 bp
  • C to A, chromosome 11 at 60,190,521 bp
  • C to A, chromosome 11 at 69,686,967 bp
  • C to A, chromosome 11 at 83,069,576 bp
  • T to C, chromosome 11 at 88,047,858 bp
  • C to A, chromosome 11 at 109,977,813 bp
  • T to C, chromosome 11 at 115,260,991 bp
  • A to G, chromosome 11 at 115,881,933 bp
  • A to G, chromosome 12 at 8,958,829 bp
  • T to A, chromosome 12 at 24,500,525 bp
  • C to A, chromosome 12 at 76,439,157 bp
  • A to G, chromosome 13 at 46,686,976 bp
  • A to G, chromosome 13 at 63,015,498 bp
  • A to T, chromosome 13 at 92,249,878 bp
  • C to T, chromosome 14 at 21,848,120 bp
  • A to G, chromosome 14 at 47,389,435 bp
  • C to A, chromosome 14 at 52,314,247 bp
  • G to A, chromosome 15 at 6,794,006 bp
  • T to A, chromosome 15 at 76,297,347 bp
  • A to G, chromosome 15 at 83,390,518 bp
  • A to T, chromosome 16 at 32,774,839 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5849 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044066-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.