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Strain Name:
C57BL/6J-MtgxR5865Btlr
Stock Number:
044074-MU
Citation ID:
RRID:MMRRC_044074-MU
Other Names:
R5865 (G1)
Major Collection:

Strain Information

Mc3r
Name: melanocortin 3 receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17201
HGNC: HGNC:6931
Homologene: 7412
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Kcnc4
Name: potassium voltage gated channel, Shaw-related subfamily, member 4
Synonyms: Kv3.4, Kcr2-4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99738
HGNC: HGNC:6236
Homologene: 68427
Nfkb1
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
Synonyms: NF kappaB1, p50 subunit of NF kappaB, p50/p105, p50, nuclear factor kappaB p50, NF-kappaB, NF-kappaB p50
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18033
HGNC: HGNC:7794
Homologene: 2971
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103554
Homologene: 113742
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214133
Homologene: 49498
Zwilch
Name: zwilch kinetochore protein
Synonyms: 2310031L18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68014
Homologene: 32381
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,471,563 bp
  • A to T, chromosome 1 at 74,146,755 bp
  • C to T, chromosome 1 at 136,151,137 bp
  • T to A, chromosome 2 at 3,225,337 bp
  • T to C, chromosome 2 at 26,293,368 bp
  • T to C, chromosome 2 at 85,904,521 bp
  • T to C, chromosome 2 at 154,266,836 bp
  • T to A, chromosome 2 at 172,249,672 bp
  • G to C, chromosome 3 at 5,402,659 bp
  • A to G, chromosome 3 at 30,679,140 bp
  • G to A, chromosome 3 at 32,712,128 bp
  • A to T, chromosome 3 at 107,458,199 bp
  • T to A, chromosome 3 at 133,487,099 bp
  • T to A, chromosome 3 at 135,603,780 bp
  • A to G, chromosome 4 at 86,586,357 bp
  • T to C, chromosome 4 at 98,771,393 bp
  • A to T, chromosome 4 at 118,621,135 bp
  • T to A, chromosome 5 at 14,714,478 bp
  • A to G, chromosome 6 at 4,754,375 bp
  • A to G, chromosome 6 at 42,596,697 bp
  • T to A, chromosome 7 at 30,474,385 bp
  • T to C, chromosome 7 at 119,975,108 bp
  • T to C, chromosome 7 at 125,575,176 bp
  • T to A, chromosome 7 at 141,650,504 bp
  • A to T, chromosome 9 at 41,983,034 bp
  • T to A, chromosome 9 at 64,172,908 bp
  • C to T, chromosome 9 at 64,191,266 bp
  • A to G, chromosome 9 at 75,297,488 bp
  • A to T, chromosome 9 at 79,604,478 bp
  • C to T, chromosome 9 at 89,085,606 bp
  • C to T, chromosome 9 at 122,853,487 bp
  • T to C, chromosome 11 at 30,791,993 bp
  • T to G, chromosome 11 at 50,803,954 bp
  • G to T, chromosome 11 at 64,997,957 bp
  • G to A, chromosome 11 at 115,126,300 bp
  • C to T, chromosome 11 at 115,990,922 bp
  • A to G, chromosome 13 at 22,219,225 bp
  • A to G, chromosome 13 at 104,236,194 bp
  • A to G, chromosome 14 at 50,928,875 bp
  • A to T, chromosome 16 at 11,122,688 bp
  • A to C, chromosome 17 at 14,955,010 bp
  • A to G, chromosome 17 at 20,584,295 bp
  • T to A, chromosome 17 at 25,491,244 bp
  • T to C, chromosome 18 at 36,961,421 bp
  • T to A, chromosome 18 at 42,536,348 bp
  • A to T, chromosome 18 at 66,083,151 bp
  • T to C, chromosome 19 at 5,496,354 bp
  • A to G, chromosome 19 at 11,304,217 bp
  • T to C, chromosome 19 at 37,910,934 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5865 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044074-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.