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Strain Name:
C57BL/6J-MtgxR5875Btlr/Mmmh
Stock Number:
044082-MU
Citation ID:
RRID:MMRRC_044082-MU
Other Names:
R5875 (G1)
Major Collection:

Strain Information

Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Lpp
Name: LIM domain containing preferred translocation partner in lipoma
Synonyms: B130055L10Rik, 9430020K16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 210126
VEGA: 16
HGNC: HGNC:6679
Homologene: 4075
Arl1
Name: ADP-ribosylation factor-like 1
Synonyms: 2310008D22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 104303
HGNC: HGNC:692
Homologene: 20319
Frmd5
Name: FERM domain containing 5
Synonyms: 1500032A09Rik, A930004K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228564
Homologene: 18206
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: 3110043H24Rik, 4930432B04Rik, D730027C18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75786
Homologene: 8844
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 38,807,047 bp
  • T to A, chromosome 1 at 87,717,974 bp
  • A to G, chromosome 2 at 26,433,367 bp
  • T to A, chromosome 2 at 37,787,254 bp
  • A to G, chromosome 2 at 67,505,080 bp
  • T to A, chromosome 2 at 72,483,557 bp
  • T to C, chromosome 2 at 74,692,082 bp
  • A to G, chromosome 2 at 91,560,861 bp
  • T to A, chromosome 2 at 119,571,815 bp
  • A to G, chromosome 2 at 121,558,478 bp
  • A to G, chromosome 2 at 127,186,817 bp
  • T to C, chromosome 3 at 36,561,193 bp
  • T to C, chromosome 3 at 95,294,814 bp
  • A to C, chromosome 3 at 144,922,889 bp
  • G to A, chromosome 4 at 3,745,631 bp
  • A to T, chromosome 4 at 59,412,203 bp
  • T to A, chromosome 4 at 123,432,314 bp
  • T to C, chromosome 4 at 124,780,406 bp
  • A to C, chromosome 4 at 153,432,341 bp
  • A to G, chromosome 5 at 4,077,285 bp
  • A to T, chromosome 5 at 14,680,600 bp
  • G to A, chromosome 5 at 21,742,946 bp
  • A to C, chromosome 5 at 100,796,470 bp
  • A to T, chromosome 5 at 108,672,037 bp
  • A to C, chromosome 5 at 110,651,310 bp
  • A to G, chromosome 5 at 120,805,562 bp
  • G to A, chromosome 5 at 124,716,327 bp
  • T to C, chromosome 6 at 73,469,045 bp
  • T to C, chromosome 6 at 88,202,491 bp
  • T to C, chromosome 6 at 129,874,828 bp
  • A to G, chromosome 7 at 13,008,310 bp
  • A to T, chromosome 7 at 41,042,936 bp
  • G to A, chromosome 7 at 42,708,046 bp
  • T to C, chromosome 7 at 79,121,973 bp
  • C to A, chromosome 7 at 127,866,302 bp
  • A to G, chromosome 8 at 70,075,389 bp
  • A to T, chromosome 8 at 91,051,836 bp
  • G to A, chromosome 8 at 105,913,099 bp
  • G to A, chromosome 9 at 35,189,169 bp
  • G to C, chromosome 9 at 75,450,560 bp
  • A to T, chromosome 10 at 78,053,938 bp
  • T to C, chromosome 10 at 88,741,979 bp
  • T to G, chromosome 10 at 116,348,166 bp
  • T to A, chromosome 10 at 118,860,697 bp
  • T to C, chromosome 11 at 6,405,072 bp
  • T to A, chromosome 11 at 61,493,698 bp
  • T to C, chromosome 11 at 86,238,312 bp
  • G to T, chromosome 11 at 113,830,059 bp
  • G to A, chromosome 11 at 117,759,708 bp
  • T to G, chromosome 13 at 34,932,446 bp
  • T to C, chromosome 13 at 58,311,104 bp
  • A to T, chromosome 13 at 84,294,858 bp
  • A to G, chromosome 13 at 93,095,184 bp
  • A to G, chromosome 13 at 95,443,796 bp
  • T to A, chromosome 13 at 119,894,094 bp
  • T to A, chromosome 14 at 25,635,966 bp
  • C to T, chromosome 14 at 30,929,530 bp
  • T to A, chromosome 15 at 100,972,822 bp
  • A to G, chromosome 16 at 14,467,037 bp
  • G to A, chromosome 16 at 24,608,309 bp
  • G to A, chromosome 16 at 32,793,819 bp
  • T to A, chromosome 17 at 19,588,830 bp
  • T to A, chromosome 17 at 56,707,955 bp
  • T to A, chromosome 18 at 40,257,394 bp
  • A to T, chromosome 18 at 74,707,902 bp
  • T to C, chromosome 18 at 77,563,181 bp
  • T to C, chromosome 19 at 5,383,795 bp
  • T to C, chromosome 19 at 8,872,220 bp
  • A to G, chromosome 19 at 30,089,302 bp
  • A to G, chromosome 19 at 41,928,103 bp
  • A to T, chromosome 19 at 47,076,110 bp
  • A to G, chromosome 19 at 55,189,626 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5875 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044082-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.