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Strain Name:
C57BL/6J-MtgxR5886Btlr/Mmmh
Stock Number:
044088-MU
Citation ID:
RRID:MMRRC_044088-MU
Other Names:
R5886 (G1)
Major Collection:

Strain Information

Tk1
Name: thymidine kinase 1
Synonyms: Tk-1, D530002A18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21877
Homologene: 37749
Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, C030040A15Rik, OTTMUSG00000012483
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109241
Homologene: 81861
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27223
Homologene: 4137
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 59,558,250 bp
  • C to T, chromosome 1 at 84,730,458 bp
  • T to C, chromosome 1 at 116,571,672 bp
  • C to T, chromosome 1 at 132,067,727 bp
  • A to G, chromosome 1 at 134,520,199 bp
  • A to T, chromosome 2 at 13,320,023 bp
  • A to T, chromosome 2 at 29,790,614 bp
  • A to G, chromosome 2 at 49,272,452 bp
  • A to G, chromosome 2 at 85,799,803 bp
  • A to G, chromosome 2 at 120,321,572 bp
  • A to G, chromosome 2 at 121,205,021 bp
  • A to T, chromosome 2 at 122,303,810 bp
  • C to A, chromosome 3 at 89,220,163 bp
  • T to A, chromosome 3 at 104,462,698 bp
  • T to A, chromosome 4 at 57,856,295 bp
  • A to G, chromosome 4 at 62,191,234 bp
  • T to C, chromosome 5 at 17,681,986 bp
  • G to A, chromosome 5 at 43,615,656 bp
  • A to G, chromosome 7 at 28,887,605 bp
  • A to G, chromosome 7 at 130,729,120 bp
  • G to T, chromosome 7 at 140,149,865 bp
  • T to C, chromosome 8 at 43,520,273 bp
  • G to A, chromosome 8 at 45,027,681 bp
  • A to T, chromosome 8 at 45,033,395 bp
  • T to A, chromosome 8 at 79,672,807 bp
  • T to C, chromosome 8 at 84,523,022 bp
  • T to A, chromosome 9 at 39,060,382 bp
  • G to T, chromosome 9 at 39,669,956 bp
  • A to G, chromosome 9 at 57,548,462 bp
  • G to A, chromosome 9 at 83,399,681 bp
  • A to G, chromosome 9 at 110,987,136 bp
  • A to T, chromosome 9 at 123,091,081 bp
  • G to A, chromosome 10 at 99,445,147 bp
  • A to G, chromosome 11 at 69,884,336 bp
  • A to G, chromosome 11 at 70,982,182 bp
  • C to T, chromosome 11 at 83,003,334 bp
  • A to G, chromosome 11 at 88,834,660 bp
  • C to T, chromosome 11 at 99,540,081 bp
  • G to A, chromosome 11 at 100,761,933 bp
  • A to G, chromosome 11 at 103,208,121 bp
  • CC to GA, chromosome 11 at 117,817,122 bp
  • T to A, chromosome 12 at 52,181,309 bp
  • T to A, chromosome 12 at 76,327,845 bp
  • T to C, chromosome 12 at 102,260,415 bp
  • C to A, chromosome 13 at 32,802,836 bp
  • A to G, chromosome 13 at 42,156,612 bp
  • A to T, chromosome 13 at 96,660,183 bp
  • C to T, chromosome 14 at 20,561,842 bp
  • C to G, chromosome 14 at 66,304,053 bp
  • T to C, chromosome 14 at 66,304,054 bp
  • T to C, chromosome 14 at 73,029,491 bp
  • A to T, chromosome 15 at 58,098,514 bp
  • G to T, chromosome 15 at 74,606,447 bp
  • A to C, chromosome 15 at 75,891,475 bp
  • A to T, chromosome 15 at 89,103,247 bp
  • T to C, chromosome 15 at 98,226,791 bp
  • T to A, chromosome 16 at 37,979,013 bp
  • T to C, chromosome 16 at 44,790,203 bp
  • C to T, chromosome 17 at 25,171,818 bp
  • T to C, chromosome 17 at 28,876,863 bp
  • C to T, chromosome 17 at 30,794,717 bp
  • A to T, chromosome 17 at 32,524,039 bp
  • T to C, chromosome 17 at 53,451,554 bp
  • T to C, chromosome 18 at 20,874,680 bp
  • T to A, chromosome 18 at 36,790,097 bp
  • T to C, chromosome 18 at 76,859,429 bp
  • A to G, chromosome 19 at 6,066,542 bp
  • T to C, chromosome 19 at 16,664,562 bp
  • C to A, chromosome 19 at 27,243,771 bp
  • T to C, chromosome 19 at 29,719,277 bp
  • C to T, chromosome 19 at 60,467,576 bp
  • T to A, chromosome 19 at 60,756,830 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5886 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044088-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.