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Strain Name:
C57BL/6J-MtgxR5898Btlr/Mmmh
Stock Number:
044097-MU
Citation ID:
RRID:MMRRC_044097-MU
Other Names:
R5898 (G1)
Major Collection:

Strain Information

Shc1
Name: src homology 2 domain-containing transforming protein C1
Synonyms: p66, ShcA, p66shc
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20416
Homologene: 7934
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Wdr47
Name: WD repeat domain 47
Synonyms: 1810073M12Rik, nemitin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99512
Homologene: 8984
Adsl
Name: adenylosuccinate lyase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11564
HGNC: HGNC:291
Homologene: 12
Usp14
Name: ubiquitin specific peptidase 14
Synonyms: dUB-type TGT, 2610005K12Rik, ataxia, ax, NMF375, 2610037B11Rik, nmf375
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 59025
Homologene: 3780
Erbin
Name: Erbb2 interacting protein
Synonyms: 1700028E05Rik, Erbin, Erbb2ip
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 59079
Homologene: 41282
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 75,186,272 bp
  • A to C, chromosome 1 at 93,479,301 bp
  • T to C, chromosome 1 at 97,744,162 bp
  • T to C, chromosome 1 at 135,585,146 bp
  • T to C, chromosome 1 at 135,882,521 bp
  • T to C, chromosome 1 at 173,568,979 bp
  • T to A, chromosome 1 at 176,824,822 bp
  • T to G, chromosome 1 at 180,421,315 bp
  • C to T, chromosome 2 at 34,997,437 bp
  • T to C, chromosome 2 at 60,422,760 bp
  • A to T, chromosome 2 at 62,573,503 bp
  • T to C, chromosome 2 at 65,514,695 bp
  • C to T, chromosome 2 at 104,845,033 bp
  • A to T, chromosome 2 at 120,745,040 bp
  • T to A, chromosome 2 at 131,073,633 bp
  • T to C, chromosome 2 at 150,839,778 bp
  • T to A, chromosome 2 at 158,204,657 bp
  • T to A, chromosome 3 at 89,426,967 bp
  • T to A, chromosome 3 at 108,637,885 bp
  • G to T, chromosome 3 at 145,016,761 bp
  • A to G, chromosome 3 at 146,427,831 bp
  • A to T, chromosome 4 at 62,326,057 bp
  • G to T, chromosome 4 at 96,577,714 bp
  • A to T, chromosome 5 at 112,802,330 bp
  • A to T, chromosome 5 at 124,386,791 bp
  • A to T, chromosome 5 at 149,625,158 bp
  • A to G, chromosome 6 at 29,467,958 bp
  • A to G, chromosome 6 at 119,274,231 bp
  • T to C, chromosome 6 at 122,580,773 bp
  • A to T, chromosome 6 at 149,513,703 bp
  • A to G, chromosome 7 at 28,288,154 bp
  • A to T, chromosome 7 at 42,662,539 bp
  • C to T, chromosome 7 at 75,729,146 bp
  • G to A, chromosome 7 at 79,433,321 bp
  • T to C, chromosome 7 at 81,537,227 bp
  • T to C, chromosome 7 at 104,950,261 bp
  • T to C, chromosome 7 at 120,078,501 bp
  • T to G, chromosome 7 at 131,241,967 bp
  • C to G, chromosome 8 at 56,541,021 bp
  • A to T, chromosome 8 at 65,000,364 bp
  • G to T, chromosome 8 at 84,098,452 bp
  • A to G, chromosome 8 at 122,487,943 bp
  • T to A, chromosome 9 at 4,330,476 bp
  • T to C, chromosome 9 at 7,148,717 bp
  • T to C, chromosome 9 at 15,938,461 bp
  • T to A, chromosome 9 at 24,758,859 bp
  • T to A, chromosome 9 at 56,207,338 bp
  • T to A, chromosome 9 at 56,885,222 bp
  • T to C, chromosome 9 at 57,630,302 bp
  • G to T, chromosome 9 at 64,685,964 bp
  • T to A, chromosome 9 at 69,408,262 bp
  • A to T, chromosome 10 at 5,643,988 bp
  • G to T, chromosome 10 at 100,612,900 bp
  • G to A, chromosome 10 at 100,615,208 bp
  • C to A, chromosome 11 at 4,144,538 bp
  • T to C, chromosome 11 at 6,617,372 bp
  • T to A, chromosome 11 at 53,873,733 bp
  • A to T, chromosome 11 at 59,546,852 bp
  • C to A, chromosome 11 at 67,192,719 bp
  • C to T, chromosome 11 at 118,114,213 bp
  • C to T, chromosome 12 at 81,104,757 bp
  • A to C, chromosome 13 at 60,782,704 bp
  • A to T, chromosome 13 at 100,299,321 bp
  • A to G, chromosome 13 at 103,839,305 bp
  • A to T, chromosome 14 at 45,602,668 bp
  • G to T, chromosome 14 at 52,811,472 bp
  • T to A, chromosome 14 at 54,363,301 bp
  • T to A, chromosome 14 at 73,161,965 bp
  • A to G, chromosome 15 at 80,961,353 bp
  • A to G, chromosome 15 at 82,455,524 bp
  • G to T, chromosome 15 at 86,100,784 bp
  • T to A, chromosome 16 at 23,491,927 bp
  • A to T, chromosome 16 at 35,422,965 bp
  • A to G, chromosome 16 at 45,544,760 bp
  • T to A, chromosome 17 at 13,125,577 bp
  • A to T, chromosome 17 at 21,314,551 bp
  • T to A, chromosome 17 at 34,182,292 bp
  • A to G, chromosome 17 at 64,625,380 bp
  • T to A, chromosome 18 at 10,022,819 bp
  • T to A, chromosome 18 at 37,357,484 bp
  • T to A, chromosome 18 at 65,307,623 bp
  • C to A, chromosome 19 at 9,013,767 bp
  • T to C, chromosome 19 at 9,018,211 bp
  • A to G, chromosome 19 at 43,760,029 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5898 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044097-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.