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Strain Name:
C57BL/6J-MtgxR5918Btlr/Mmmh
Stock Number:
044115-MU
Citation ID:
RRID:MMRRC_044115-MU
Other Names:
R5918 (G1)
Major Collection:

Strain Information

Ppp1r37
Name: protein phosphatase 1, regulatory subunit 37
Synonyms: Lrrc68
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232947
Homologene: 17851
Lactb2
Name: lactamase, beta 2
Synonyms: E430032H21Rik, Cgi-83
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 212442
Homologene: 9349
Senp6
Name: SUMO/sentrin specific peptidase 6
Synonyms: E130319N12Rik, 2810017C20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 215351
Homologene: 9196
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Gpa33
Name: glycoprotein A33 transmembrane
Synonyms: A33 antigen, 2210401D16Rik, 2010310L10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 59290
HGNC: HGNC:4445
Homologene: 4245
Sipa1l3
Name: signal-induced proliferation-associated 1 like 3
Synonyms: 2610511M17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74206
Homologene: 77938
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 13,650,730 bp
  • T to C, chromosome 1 at 46,221,643 bp
  • T to A, chromosome 1 at 60,267,892 bp
  • T to C, chromosome 1 at 166,130,538 bp
  • G to T, chromosome 1 at 188,356,814 bp
  • A to C, chromosome 2 at 36,251,077 bp
  • T to C, chromosome 2 at 38,739,091 bp
  • A to T, chromosome 2 at 45,111,259 bp
  • T to C, chromosome 2 at 52,197,894 bp
  • T to C, chromosome 2 at 73,156,784 bp
  • T to C, chromosome 2 at 76,749,954 bp
  • G to T, chromosome 2 at 121,164,037 bp
  • A to G, chromosome 2 at 177,769,706 bp
  • A to G, chromosome 3 at 88,636,080 bp
  • C to T, chromosome 3 at 108,752,387 bp
  • C to T, chromosome 4 at 58,069,345 bp
  • A to G, chromosome 4 at 126,814,271 bp
  • A to T, chromosome 5 at 35,974,525 bp
  • C to T, chromosome 5 at 51,463,237 bp
  • G to A, chromosome 5 at 105,608,251 bp
  • T to C, chromosome 5 at 110,615,466 bp
  • T to G, chromosome 5 at 142,487,602 bp
  • A to G, chromosome 6 at 29,285,943 bp
  • G to T, chromosome 7 at 17,159,745 bp
  • G to T, chromosome 7 at 19,532,111 bp
  • T to G, chromosome 7 at 23,808,947 bp
  • T to C, chromosome 7 at 29,009,152 bp
  • T to C, chromosome 7 at 29,397,206 bp
  • A to G, chromosome 7 at 44,991,464 bp
  • A to C, chromosome 7 at 72,228,540 bp
  • T to A, chromosome 7 at 107,106,621 bp
  • G to T, chromosome 8 at 91,090,130 bp
  • C to T, chromosome 8 at 94,098,303 bp
  • A to T, chromosome 8 at 110,636,472 bp
  • T to C, chromosome 9 at 19,137,388 bp
  • T to A, chromosome 9 at 45,175,116 bp
  • T to C, chromosome 9 at 80,114,116 bp
  • T to G, chromosome 10 at 81,277,912 bp
  • C to G, chromosome 10 at 93,570,886 bp
  • C to T, chromosome 11 at 61,737,044 bp
  • C to A, chromosome 11 at 65,834,199 bp
  • C to T, chromosome 11 at 68,525,671 bp
  • T to A, chromosome 11 at 74,083,260 bp
  • T to C, chromosome 11 at 74,196,118 bp
  • T to C, chromosome 11 at 79,569,222 bp
  • T to C, chromosome 11 at 97,533,497 bp
  • C to T, chromosome 11 at 99,073,671 bp
  • A to G, chromosome 11 at 116,657,540 bp
  • A to G, chromosome 12 at 110,976,766 bp
  • A to C, chromosome 12 at 113,153,667 bp
  • A to G, chromosome 14 at 50,186,968 bp
  • G to T, chromosome 14 at 60,772,288 bp
  • A to T, chromosome 15 at 7,159,416 bp
  • A to G, chromosome 15 at 33,028,167 bp
  • A to T, chromosome 15 at 98,912,427 bp
  • C to T, chromosome 16 at 5,104,901 bp
  • T to C, chromosome 17 at 18,325,768 bp
  • T to A, chromosome 17 at 22,850,981 bp
  • C to A, chromosome 19 at 7,910,046 bp
  • T to A, chromosome 19 at 13,919,775 bp
  • T to C, chromosome 19 at 20,378,493 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5918 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044115-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.