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Strain Name:
C57BL/6J-MtgxR5926Btlr/Mmmh
Stock Number:
044121-MU
Citation ID:
RRID:MMRRC_044121-MU
Other Names:
R5926 (G1)
Major Collection:

Strain Information

Hfe
Name: homeostatic iron regulator
Synonyms: MR2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15216
HGNC: HGNC:4886
Homologene: 88330
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Mark3
Name: MAP/microtubule affinity regulating kinase 3
Synonyms: ETK-1, 1600015G02Rik, A430080F22Rik, C-TAK1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17169
VEGA: 12
HGNC: HGNC:6897
Homologene: 55653
Cyb5r4
Name: cytochrome b5 reductase 4
Synonyms: B5+B5R, b5/b5r, 2810034J18Rik, Ncb5or
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 266690
Homologene: 69207
Kcnn2
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2
Synonyms: small conductance calcium-activated potassium channel 2, SK2, fri, bc
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 140492
HGNC: HGNC:6291
Homologene: 135651
Doc2a
Name: double C2, alpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13446
HGNC: HGNC:2985
Homologene: 2657
Notch1
Name: notch 1
Synonyms: lin-12, Tan1, Mis6, 9930111A19Rik, Motch A, N1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18128
HGNC: HGNC:7881
Homologene: 32049
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 15,313,011 bp
  • T to A, chromosome 1 at 60,363,242 bp
  • G to A, chromosome 1 at 75,254,598 bp
  • T to A, chromosome 1 at 88,330,747 bp
  • A to T, chromosome 1 at 92,491,566 bp
  • A to G, chromosome 1 at 192,413,077 bp
  • T to C, chromosome 2 at 12,348,100 bp
  • A to G, chromosome 2 at 26,476,104 bp
  • C to T, chromosome 2 at 32,315,804 bp
  • T to A, chromosome 2 at 130,443,556 bp
  • T to C, chromosome 2 at 161,564,686 bp
  • T to A, chromosome 2 at 168,706,271 bp
  • A to G, chromosome 3 at 94,998,996 bp
  • A to T, chromosome 4 at 61,833,049 bp
  • A to T, chromosome 4 at 86,599,316 bp
  • T to C, chromosome 4 at 86,874,568 bp
  • C to T, chromosome 5 at 37,085,280 bp
  • A to G, chromosome 5 at 150,534,622 bp
  • A to G, chromosome 6 at 21,217,085 bp
  • T to C, chromosome 6 at 115,619,898 bp
  • T to C, chromosome 6 at 125,604,174 bp
  • T to A, chromosome 6 at 128,560,645 bp
  • A to G, chromosome 7 at 29,104,360 bp
  • G to T, chromosome 7 at 45,725,295 bp
  • G to T, chromosome 7 at 84,343,946 bp
  • T to C, chromosome 7 at 107,933,903 bp
  • T to C, chromosome 7 at 108,713,587 bp
  • G to A, chromosome 7 at 126,784,853 bp
  • T to C, chromosome 7 at 126,849,525 bp
  • A to G, chromosome 9 at 54,338,438 bp
  • C to T, chromosome 9 at 87,057,261 bp
  • T to C, chromosome 9 at 106,838,362 bp
  • C to T, chromosome 10 at 79,902,528 bp
  • A to G, chromosome 11 at 68,951,955 bp
  • T to C, chromosome 11 at 80,478,302 bp
  • C to T, chromosome 11 at 100,768,237 bp
  • G to A, chromosome 12 at 29,832,332 bp
  • T to A, chromosome 12 at 72,077,185 bp
  • T to A, chromosome 12 at 111,592,734 bp
  • A to T, chromosome 13 at 23,708,264 bp
  • A to T, chromosome 13 at 55,534,299 bp
  • A to G, chromosome 13 at 63,545,055 bp
  • A to G, chromosome 13 at 64,920,725 bp
  • T to A, chromosome 14 at 37,055,009 bp
  • T to A, chromosome 15 at 41,866,254 bp
  • A to G, chromosome 18 at 45,685,284 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5926 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044121-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.