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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR5930Btlr/Mmmh
Stock Number:
044125-MU
Citation ID:
RRID:MMRRC_044125-MU
Other Names:
R5930 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Gabra6
Name: gamma-aminobutyric acid type A receptor subunit alpha 6
Synonyms: Gabra-6, alpha6, GABA-ARalpha6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14399
HGNC: HGNC:4080
Homologene: 20220

Additional Resources
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Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Upf1
Name: UPF1 RNA helicase and ATPase
Synonyms: PNORF-1, B430202H16Rik, Rent1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19704
HGNC: HGNC:9962
Homologene: 2185

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Agap1
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Ggap1, Centg2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 347722
Homologene: 56689

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,385,173 bp
  • T to A, chromosome 1 at 61,768,130 bp
  • C to T, chromosome 1 at 89,843,096 bp
  • T to A, chromosome 1 at 132,523,432 bp
  • A to T, chromosome 1 at 162,839,616 bp
  • A to G, chromosome 1 at 163,997,177 bp
  • G to T, chromosome 1 at 191,009,551 bp
  • T to A, chromosome 2 at 12,230,208 bp
  • T to C, chromosome 2 at 76,139,831 bp
  • A to T, chromosome 2 at 125,095,731 bp
  • C to T, chromosome 2 at 127,530,133 bp
  • GGCCG to GG, chromosome 2 at 162,967,336 bp
  • T to C, chromosome 3 at 80,707,249 bp
  • T to C, chromosome 3 at 94,436,410 bp
  • A to T, chromosome 3 at 108,980,152 bp
  • T to C, chromosome 4 at 18,886,119 bp
  • T to A, chromosome 4 at 25,214,756 bp
  • A to G, chromosome 4 at 109,430,878 bp
  • C to A, chromosome 4 at 115,491,410 bp
  • C to T, chromosome 4 at 118,809,378 bp
  • A to T, chromosome 4 at 133,871,571 bp
  • A to G, chromosome 4 at 144,272,983 bp
  • A to G, chromosome 4 at 148,156,595 bp
  • T to C, chromosome 5 at 124,791,791 bp
  • C to T, chromosome 5 at 142,721,402 bp
  • A to T, chromosome 6 at 5,157,002 bp
  • A to G, chromosome 6 at 65,605,953 bp
  • T to G, chromosome 6 at 124,715,649 bp
  • C to T, chromosome 6 at 130,053,053 bp
  • T to C, chromosome 6 at 146,603,911 bp
  • T to A, chromosome 7 at 25,326,441 bp
  • A to G, chromosome 7 at 27,174,502 bp
  • T to A, chromosome 7 at 46,443,464 bp
  • T to C, chromosome 7 at 51,585,331 bp
  • A to G, chromosome 7 at 80,253,166 bp
  • A to T, chromosome 7 at 96,854,719 bp
  • A to T, chromosome 7 at 102,804,274 bp
  • A to G, chromosome 7 at 104,154,155 bp
  • T to A, chromosome 7 at 104,421,450 bp
  • T to A, chromosome 7 at 127,846,489 bp
  • A to G, chromosome 7 at 127,905,973 bp
  • C to T, chromosome 7 at 140,230,446 bp
  • T to A, chromosome 8 at 13,837,263 bp
  • T to A, chromosome 8 at 14,896,621 bp
  • A to G, chromosome 8 at 25,182,199 bp
  • A to G, chromosome 8 at 45,044,036 bp
  • G to A, chromosome 8 at 70,344,262 bp
  • G to A, chromosome 8 at 78,436,391 bp
  • A to G, chromosome 8 at 93,896,245 bp
  • A to G, chromosome 8 at 110,710,349 bp
  • A to T, chromosome 8 at 121,611,358 bp
  • C to T, chromosome 8 at 123,460,070 bp
  • A to G, chromosome 9 at 19,920,910 bp
  • A to G, chromosome 9 at 21,824,416 bp
  • A to T, chromosome 9 at 31,103,760 bp
  • T to C, chromosome 9 at 72,971,998 bp
  • A to C, chromosome 9 at 109,030,394 bp
  • C to T, chromosome 9 at 110,887,364 bp
  • T to C, chromosome 10 at 7,142,993 bp
  • G to T, chromosome 10 at 59,130,986 bp
  • T to A, chromosome 10 at 118,860,268 bp
  • T to C, chromosome 10 at 128,801,454 bp
  • G to A, chromosome 11 at 8,958,969 bp
  • G to A, chromosome 11 at 42,307,441 bp
  • C to T, chromosome 11 at 69,834,149 bp
  • A to T, chromosome 11 at 72,712,245 bp
  • T to C, chromosome 11 at 78,937,915 bp
  • C to A, chromosome 11 at 83,863,985 bp
  • G to A, chromosome 11 at 84,819,192 bp
  • T to A, chromosome 11 at 99,785,636 bp
  • A to T, chromosome 11 at 100,893,670 bp
  • G to T, chromosome 11 at 107,073,196 bp
  • T to A, chromosome 12 at 83,677,866 bp
  • A to G, chromosome 13 at 15,548,625 bp
  • T to A, chromosome 13 at 23,486,228 bp
  • C to T, chromosome 13 at 49,589,636 bp
  • C to T, chromosome 13 at 59,727,015 bp
  • C to T, chromosome 13 at 81,397,451 bp
  • T to A, chromosome 14 at 27,776,858 bp
  • C to T, chromosome 14 at 31,173,145 bp
  • C to A, chromosome 14 at 50,515,792 bp
  • T to C, chromosome 15 at 79,303,528 bp
  • T to C, chromosome 15 at 81,237,843 bp
  • T to A, chromosome 15 at 85,086,121 bp
  • A to T, chromosome 16 at 32,751,705 bp
  • T to A, chromosome 16 at 62,928,061 bp
  • A to T, chromosome 17 at 24,718,030 bp
  • T to C, chromosome 17 at 25,943,719 bp
  • A to T, chromosome 17 at 27,110,921 bp
  • G to A, chromosome 17 at 32,629,983 bp
  • C to T, chromosome 17 at 36,862,360 bp
  • C to A, chromosome 17 at 38,067,750 bp
  • T to A, chromosome 17 at 56,769,579 bp
  • A to C, chromosome 18 at 37,491,935 bp
  • A to G, chromosome 18 at 39,150,092 bp
  • A to G, chromosome 18 at 40,530,829 bp
  • A to G, chromosome 18 at 64,478,649 bp
  • T to A, chromosome 19 at 3,902,424 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5930 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
044125-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.