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Strain Name:
C57BL/6J-MtgxR5931Btlr/Mmmh
Stock Number:
044126-MU
Citation ID:
RRID:MMRRC_044126-MU
Other Names:
R5931 (G1)
Major Collection:

Strain Information

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Cdh1
Name: cadherin 1
Synonyms: E-cadherin, Ecad, UM, uvomorulin, L-CAM, E-cad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12550
HGNC: HGNC:1748
Homologene: 20917
Gabra6
Name: gamma-aminobutyric acid type A receptor subunit alpha 6
Synonyms: Gabra-6, alpha6, GABA-ARalpha6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14399
HGNC: HGNC:4080
Homologene: 20220
Ppp1cb
Name: protein phosphatase 1 catalytic subunit beta
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19046
HGNC: HGNC:9282
Homologene: 37659
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: Lcmt, LCMT-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 30949
Homologene: 41123
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,350,674 bp
  • C to T, chromosome 1 at 85,679,083 bp
  • T to C, chromosome 1 at 106,777,335 bp
  • TTGCTGCTGCTGCTGCTGC to TTGCTGCTGCTGCTGC, chromosome 1 at 132,357,755 bp
  • T to C, chromosome 1 at 156,656,487 bp
  • A to T, chromosome 1 at 194,810,870 bp
  • C to T, chromosome 2 at 73,568,100 bp
  • A to T, chromosome 2 at 122,178,594 bp
  • G to A, chromosome 2 at 130,814,189 bp
  • A to T, chromosome 2 at 130,933,436 bp
  • C to T, chromosome 2 at 181,330,815 bp
  • A to T, chromosome 3 at 116,523,425 bp
  • A to C, chromosome 3 at 145,092,125 bp
  • T to C, chromosome 4 at 116,590,260 bp
  • A to C, chromosome 4 at 134,507,720 bp
  • T to A, chromosome 5 at 32,483,466 bp
  • A to G, chromosome 5 at 110,735,520 bp
  • C to T, chromosome 5 at 111,085,109 bp
  • T to C, chromosome 5 at 120,963,873 bp
  • T to A, chromosome 5 at 130,229,508 bp
  • A to G, chromosome 6 at 49,469,302 bp
  • G to A, chromosome 6 at 52,172,588 bp
  • A to G, chromosome 6 at 80,021,739 bp
  • A to T, chromosome 6 at 91,919,115 bp
  • C to T, chromosome 6 at 130,053,053 bp
  • C to A, chromosome 7 at 16,960,884 bp
  • A to G, chromosome 7 at 123,421,616 bp
  • A to T, chromosome 8 at 48,646,498 bp
  • G to T, chromosome 8 at 84,995,746 bp
  • A to G, chromosome 8 at 88,729,842 bp
  • T to C, chromosome 8 at 106,666,332 bp
  • T to C, chromosome 9 at 15,561,322 bp
  • A to T, chromosome 9 at 19,537,333 bp
  • A to G, chromosome 9 at 38,116,374 bp
  • T to C, chromosome 9 at 60,872,012 bp
  • T to A, chromosome 9 at 104,020,746 bp
  • T to C, chromosome 10 at 76,471,166 bp
  • G to A, chromosome 11 at 42,307,441 bp
  • A to G, chromosome 11 at 101,261,221 bp
  • A to G, chromosome 11 at 106,426,873 bp
  • T to G, chromosome 11 at 108,014,310 bp
  • C to A, chromosome 12 at 8,341,915 bp
  • T to A, chromosome 12 at 76,008,865 bp
  • C to T, chromosome 12 at 85,222,787 bp
  • T to A, chromosome 12 at 113,260,090 bp
  • A to G, chromosome 13 at 97,099,197 bp
  • T to A, chromosome 13 at 115,088,843 bp
  • A to C, chromosome 14 at 55,498,940 bp
  • A to G, chromosome 14 at 57,696,131 bp
  • A to T, chromosome 14 at 72,568,867 bp
  • A to G, chromosome 14 at 73,238,928 bp
  • T to A, chromosome 14 at 103,605,624 bp
  • G to A, chromosome 15 at 7,243,857 bp
  • A to T, chromosome 15 at 28,453,279 bp
  • T to A, chromosome 16 at 17,857,309 bp
  • G to A, chromosome 16 at 22,558,038 bp
  • A to T, chromosome 16 at 32,794,574 bp
  • A to G, chromosome 17 at 34,729,193 bp
  • G to A, chromosome 17 at 56,780,778 bp
  • A to T, chromosome 18 at 36,954,755 bp
  • T to A, chromosome 18 at 44,872,956 bp
  • T to A, chromosome 18 at 84,981,125 bp
  • C to G, chromosome 19 at 29,615,729 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5931 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044126-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.