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Strain Name:
C57BL/6J-MtgxR5945Btlr/Mmmh
Stock Number:
044137-MU
Citation ID:
RRID:MMRRC_044137-MU
Other Names:
R5945 (G1)
Major Collection:

Strain Information

Itpk1
Name: inositol 1,3,4-triphosphate 5/6 kinase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217837
HGNC: HGNC:6177
Homologene: 8588
Apobec3
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3
Synonyms: Rfv-3, CEM15, Rfv3, Gm20117
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 80287
Homologene: 105420
Crhr2
Name: corticotropin releasing hormone receptor 2
Synonyms: Crfr2, CRF-R2, CRFR2beta, CRFR2alpha, CRF 2 receptor, CRH-R2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12922
HGNC: HGNC:2358
Homologene: 55612
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Epb41l4a
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: NBL4, Epb4.1l4a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13824
VEGA: 18
Homologene: 8398
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Arhgef10
Name: Rho guanine nucleotide exchange factor 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234094
Homologene: 22827
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 10,038,010 bp
  • T to C, chromosome 1 at 14,898,135 bp
  • C to T, chromosome 1 at 72,192,200 bp
  • T to A, chromosome 1 at 151,411,170 bp
  • T to C, chromosome 2 at 23,039,965 bp
  • T to G, chromosome 2 at 25,655,497 bp
  • T to A, chromosome 2 at 28,859,890 bp
  • T to C, chromosome 2 at 30,828,203 bp
  • C to A, chromosome 2 at 53,114,199 bp
  • A to G, chromosome 2 at 60,240,987 bp
  • A to T, chromosome 2 at 87,962,602 bp
  • A to G, chromosome 2 at 104,066,616 bp
  • A to G, chromosome 3 at 38,983,206 bp
  • A to T, chromosome 3 at 93,445,337 bp
  • C to T, chromosome 3 at 158,023,344 bp
  • A to T, chromosome 4 at 19,283,579 bp
  • G to A, chromosome 4 at 41,428,670 bp
  • T to C, chromosome 4 at 108,021,713 bp
  • T to A, chromosome 4 at 115,756,467 bp
  • T to A, chromosome 4 at 136,287,217 bp
  • T to C, chromosome 4 at 136,568,701 bp
  • T to A, chromosome 4 at 143,437,723 bp
  • T to A, chromosome 4 at 145,538,043 bp
  • G to T, chromosome 4 at 152,379,951 bp
  • A to G, chromosome 5 at 36,485,850 bp
  • C to T, chromosome 5 at 90,786,316 bp
  • A to T, chromosome 5 at 110,682,866 bp
  • A to G, chromosome 5 at 140,353,480 bp
  • G to A, chromosome 5 at 143,324,886 bp
  • T to A, chromosome 6 at 55,100,682 bp
  • T to G, chromosome 6 at 83,037,511 bp
  • T to A, chromosome 6 at 141,994,282 bp
  • T to A, chromosome 6 at 145,619,745 bp
  • C to A, chromosome 7 at 27,702,416 bp
  • T to C, chromosome 7 at 41,215,612 bp
  • T to C, chromosome 7 at 98,619,383 bp
  • T to C, chromosome 8 at 14,980,028 bp
  • T to C, chromosome 8 at 64,749,728 bp
  • T to C, chromosome 8 at 72,731,165 bp
  • T to C, chromosome 8 at 72,852,200 bp
  • T to A, chromosome 8 at 93,363,626 bp
  • A to G, chromosome 9 at 4,456,122 bp
  • T to A, chromosome 9 at 72,362,332 bp
  • T to C, chromosome 9 at 103,046,790 bp
  • A to G, chromosome 9 at 110,384,596 bp
  • A to G, chromosome 9 at 121,584,463 bp
  • G to A, chromosome 10 at 67,267,520 bp
  • C to T, chromosome 11 at 9,293,398 bp
  • A to T, chromosome 11 at 53,996,028 bp
  • T to C, chromosome 11 at 76,234,471 bp
  • T to C, chromosome 11 at 100,745,322 bp
  • T to C, chromosome 12 at 3,500,439 bp
  • T to C, chromosome 12 at 51,629,496 bp
  • C to A, chromosome 12 at 84,926,347 bp
  • A to G, chromosome 12 at 102,588,553 bp
  • A to T, chromosome 13 at 11,660,122 bp
  • A to G, chromosome 13 at 55,628,632 bp
  • A to T, chromosome 13 at 63,573,419 bp
  • T to G, chromosome 13 at 114,966,590 bp
  • A to G, chromosome 14 at 50,148,763 bp
  • A to G, chromosome 14 at 54,514,887 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • T to A, chromosome 15 at 79,365,481 bp
  • C to T, chromosome 15 at 79,897,846 bp
  • T to C, chromosome 15 at 89,109,217 bp
  • T to A, chromosome 15 at 91,245,777 bp
  • T to C, chromosome 15 at 93,455,532 bp
  • C to T, chromosome 15 at 102,118,195 bp
  • G to A, chromosome 16 at 4,545,629 bp
  • T to C, chromosome 16 at 17,894,701 bp
  • C to A, chromosome 16 at 29,237,243 bp
  • A to G, chromosome 16 at 34,723,588 bp
  • A to G, chromosome 16 at 44,099,946 bp
  • A to G, chromosome 16 at 59,168,119 bp
  • C to T, chromosome 16 at 59,185,728 bp
  • T to C, chromosome 17 at 14,106,339 bp
  • C to G, chromosome 17 at 20,959,413 bp
  • A to G, chromosome 17 at 33,932,038 bp
  • T to C, chromosome 17 at 36,460,626 bp
  • A to G, chromosome 17 at 57,301,870 bp
  • T to A, chromosome 18 at 33,828,730 bp
  • G to A, chromosome 18 at 37,321,470 bp
  • G to T, chromosome 19 at 5,891,866 bp
  • A to C, chromosome 19 at 11,480,499 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5945 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044137-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.