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Strain Name:
C57BL/6J-MtgxR5952Btlr/Mmmh
Stock Number:
044142-MU
Citation ID:
RRID:MMRRC_044142-MU
Other Names:
R5952 (G1)
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Epb41l1
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13821
HGNC: HGNC:3378
Homologene: 8126
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Ddx23
Name: DEAD box helicase 23
Synonyms: 4921506D17Rik, 3110082M05Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 74351
Homologene: 3542
Hira
Name: histone cell cycle regulator
Synonyms: Tuple1, D16Ertd95e, Gm15797
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 15260
HGNC: HGNC:4916
Homologene: 48172
Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 6,248,182 bp
  • G to A, chromosome 1 at 39,999,922 bp
  • T to C, chromosome 1 at 127,822,740 bp
  • A to G, chromosome 1 at 143,745,134 bp
  • A to G, chromosome 1 at 178,475,874 bp
  • A to G, chromosome 1 at 190,885,420 bp
  • A to G, chromosome 1 at 193,332,362 bp
  • T to C, chromosome 2 at 76,880,225 bp
  • T to A, chromosome 2 at 156,503,788 bp
  • G to T, chromosome 2 at 156,524,983 bp
  • A to T, chromosome 2 at 160,782,017 bp
  • A to G, chromosome 3 at 5,396,970 bp
  • A to G, chromosome 3 at 36,965,621 bp
  • A to T, chromosome 3 at 136,928,571 bp
  • C to T, chromosome 4 at 115,414,517 bp
  • C to A, chromosome 4 at 149,130,201 bp
  • A to G, chromosome 6 at 23,247,428 bp
  • T to C, chromosome 6 at 40,491,542 bp
  • A to G, chromosome 6 at 41,107,219 bp
  • G to A, chromosome 6 at 55,679,017 bp
  • A to T, chromosome 6 at 86,109,746 bp
  • G to A, chromosome 7 at 5,093,912 bp
  • C to T, chromosome 7 at 43,355,927 bp
  • T to C, chromosome 7 at 99,925,633 bp
  • T to A, chromosome 7 at 103,597,967 bp
  • T to C, chromosome 7 at 127,487,752 bp
  • G to T, chromosome 8 at 25,181,995 bp
  • A to T, chromosome 8 at 47,496,917 bp
  • A to T, chromosome 10 at 28,585,675 bp
  • A to G, chromosome 10 at 30,560,842 bp
  • A to G, chromosome 10 at 41,357,563 bp
  • A to G, chromosome 10 at 63,015,595 bp
  • A to T, chromosome 10 at 69,986,463 bp
  • A to T, chromosome 11 at 49,134,572 bp
  • A to G, chromosome 11 at 60,479,420 bp
  • G to T, chromosome 11 at 78,190,268 bp
  • A to T, chromosome 12 at 11,260,896 bp
  • T to G, chromosome 12 at 48,555,936 bp
  • A to G, chromosome 12 at 71,063,206 bp
  • A to T, chromosome 12 at 76,632,384 bp
  • A to T, chromosome 12 at 114,366,584 bp
  • A to T, chromosome 13 at 23,482,808 bp
  • A to G, chromosome 15 at 47,733,505 bp
  • A to G, chromosome 15 at 77,773,332 bp
  • A to G, chromosome 15 at 83,572,240 bp
  • A to T, chromosome 15 at 98,658,240 bp
  • C to A, chromosome 16 at 18,935,065 bp
  • A to G, chromosome 18 at 4,674,554 bp
  • T to C, chromosome 18 at 24,701,387 bp
  • T to C, chromosome 18 at 37,487,080 bp
  • T to C, chromosome 18 at 37,638,282 bp
  • A to G, chromosome 18 at 67,506,709 bp
  • T to A, chromosome 19 at 10,964,122 bp
  • T to C, chromosome 19 at 12,919,830 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5952 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044142-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.