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Strain Name:
C57BL/6J-MtgxR5977Btlr/Mmmh
Stock Number:
044159-MU
Citation ID:
RRID:MMRRC_044159-MU
Other Names:
R5977 (G1)
Major Collection:

Strain Information

Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Cr1l
Name: complement C3b/C4b receptor 1 like
Synonyms: mCRY, Crry
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12946
Homologene: 128275
Cbfa2t2
Name: CBFA2/RUNX1 translocation partner 2
Synonyms: MTGR1, C330013D05Rik, Cbfa2t2h
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12396
HGNC: HGNC:1536
Homologene: 3733
Cemip2
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 83921
VEGA: 19
Homologene: 75008
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Arl15
Name: ADP-ribosylation factor-like 15
Synonyms: C230032K13Rik, Arfrp2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218639
Homologene: 56843
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,960,218 bp
  • T to G, chromosome 1 at 34,502,702 bp
  • C to T, chromosome 1 at 88,216,260 bp
  • A to G, chromosome 1 at 90,821,849 bp
  • A to T, chromosome 1 at 195,114,768 bp
  • T to G, chromosome 2 at 90,906,143 bp
  • T to C, chromosome 2 at 154,517,777 bp
  • A to T, chromosome 3 at 8,666,358 bp
  • C to T, chromosome 3 at 64,716,043 bp
  • G to A, chromosome 4 at 44,894,982 bp
  • C to A, chromosome 4 at 47,035,748 bp
  • C to T, chromosome 4 at 128,059,034 bp
  • T to C, chromosome 4 at 143,417,659 bp
  • T to C, chromosome 5 at 9,140,451 bp
  • A to G, chromosome 5 at 33,389,860 bp
  • A to G, chromosome 5 at 67,747,285 bp
  • A to G, chromosome 5 at 115,130,553 bp
  • T to C, chromosome 6 at 22,254,608 bp
  • T to A, chromosome 6 at 39,383,926 bp
  • T to C, chromosome 6 at 113,482,731 bp
  • A to G, chromosome 6 at 128,581,122 bp
  • G to A, chromosome 7 at 10,580,490 bp
  • A to T, chromosome 7 at 12,554,737 bp
  • A to G, chromosome 7 at 12,632,216 bp
  • G to A, chromosome 7 at 50,600,090 bp
  • A to G, chromosome 7 at 85,090,548 bp
  • T to A, chromosome 7 at 86,920,333 bp
  • T to A, chromosome 7 at 86,954,907 bp
  • A to G, chromosome 7 at 100,863,148 bp
  • T to A, chromosome 7 at 110,377,986 bp
  • A to G, chromosome 7 at 118,141,357 bp
  • T to C, chromosome 7 at 141,796,367 bp
  • G to A, chromosome 8 at 62,021,410 bp
  • ATTGCTGTTT to ATTGCTGTTTGCTGTTT, chromosome 9 at 38,026,540 bp
  • A to C, chromosome 9 at 45,721,298 bp
  • T to A, chromosome 9 at 66,433,322 bp
  • A to T, chromosome 10 at 78,847,738 bp
  • A to T, chromosome 10 at 100,615,244 bp
  • C to A, chromosome 10 at 127,750,879 bp
  • C to T, chromosome 10 at 128,382,697 bp
  • A to C, chromosome 11 at 4,040,055 bp
  • G to A, chromosome 11 at 49,340,765 bp
  • C to T, chromosome 11 at 60,852,502 bp
  • T to C, chromosome 11 at 69,520,881 bp
  • C to T, chromosome 11 at 78,167,825 bp
  • G to A, chromosome 12 at 83,784,369 bp
  • A to C, chromosome 12 at 114,946,209 bp
  • A to C, chromosome 13 at 22,383,246 bp
  • C to T, chromosome 13 at 24,069,736 bp
  • A to G, chromosome 13 at 41,103,127 bp
  • G to A, chromosome 13 at 67,591,621 bp
  • C to T, chromosome 13 at 81,435,559 bp
  • T to C, chromosome 13 at 100,611,689 bp
  • T to G, chromosome 13 at 113,934,109 bp
  • G to T, chromosome 14 at 57,382,779 bp
  • T to A, chromosome 15 at 7,318,245 bp
  • T to A, chromosome 15 at 8,130,237 bp
  • A to G, chromosome 15 at 34,099,574 bp
  • C to T, chromosome 15 at 83,629,488 bp
  • C to G, chromosome 16 at 12,267,428 bp
  • T to G, chromosome 16 at 36,042,004 bp
  • T to C, chromosome 16 at 49,153,503 bp
  • T to C, chromosome 17 at 74,603,036 bp
  • A to G, chromosome 18 at 37,485,667 bp
  • A to G, chromosome 18 at 52,869,321 bp
  • T to G, chromosome 19 at 21,557,596 bp
  • A to G, chromosome 19 at 21,826,083 bp
  • A to G, chromosome 19 at 42,735,472 bp
  • T to C, chromosome 19 at 48,796,450 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5977 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044159-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.