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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR5988Btlr/Mmmh
Stock Number:
044168-MU
Citation ID:
RRID:MMRRC_044168-MU
Other Names:
R5988 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Dnajc3
Name: DnaJ heat shock protein family (Hsp40) member C3
Synonyms: p58IPK, mp58, Prkri, Dnajc3, Dnajc3a, Dnajc3b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100037258
VEGA: 14
HGNC: HGNC:9439
Homologene: 2486

Additional Resources
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Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Acvr1c
Name: activin A receptor, type IC
Synonyms: ALK7, Alk-7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269275
Homologene: 26724

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Il21r
Name: interleukin 21 receptor
Synonyms: NILR
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 60504
HGNC: HGNC:6006
Homologene: 11040

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, neurexin II alpha, 6430591O13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: PTP36, C130080N23Rik, OTTMUSG00000022087
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 22,596,463 bp
  • G to A, chromosome 1 at 78,499,206 bp
  • T to A, chromosome 1 at 172,957,156 bp
  • A to C, chromosome 1 at 173,481,340 bp
  • G to T, chromosome 1 at 186,704,581 bp
  • A to G, chromosome 1 at 189,850,387 bp
  • A to G, chromosome 2 at 13,582,485 bp
  • T to A, chromosome 2 at 28,452,554 bp
  • A to G, chromosome 2 at 36,937,224 bp
  • T to A, chromosome 2 at 49,946,166 bp
  • A to T, chromosome 2 at 58,315,874 bp
  • C to T, chromosome 2 at 66,726,214 bp
  • T to C, chromosome 2 at 76,969,788 bp
  • T to C, chromosome 2 at 90,689,209 bp
  • A to G, chromosome 2 at 156,307,330 bp
  • A to T, chromosome 2 at 180,594,233 bp
  • T to G, chromosome 3 at 8,666,319 bp
  • A to C, chromosome 3 at 107,895,954 bp
  • G to A, chromosome 4 at 49,502,871 bp
  • A to G, chromosome 4 at 81,284,575 bp
  • A to T, chromosome 4 at 144,273,146 bp
  • T to C, chromosome 4 at 144,453,205 bp
  • A to G, chromosome 4 at 148,668,507 bp
  • T to A, chromosome 5 at 21,660,583 bp
  • A to G, chromosome 5 at 25,311,120 bp
  • C to A, chromosome 5 at 32,830,851 bp
  • A to G, chromosome 5 at 101,884,138 bp
  • A to T, chromosome 5 at 145,794,918 bp
  • C to T, chromosome 6 at 85,809,058 bp
  • G to A, chromosome 6 at 89,357,540 bp
  • A to C, chromosome 6 at 94,628,042 bp
  • T to A, chromosome 6 at 131,678,643 bp
  • T to C, chromosome 6 at 138,043,531 bp
  • T to C, chromosome 7 at 5,115,502 bp
  • A to G, chromosome 7 at 28,294,112 bp
  • T to C, chromosome 7 at 44,540,580 bp
  • T to C, chromosome 7 at 48,874,995 bp
  • T to A, chromosome 7 at 64,226,805 bp
  • A to G, chromosome 7 at 80,876,199 bp
  • A to T, chromosome 7 at 104,916,918 bp
  • C to A, chromosome 7 at 111,077,230 bp
  • T to A, chromosome 7 at 123,182,380 bp
  • T to C, chromosome 7 at 125,632,288 bp
  • G to A, chromosome 8 at 15,041,854 bp
  • A to G, chromosome 8 at 22,062,028 bp
  • G to A, chromosome 8 at 25,748,562 bp
  • A to G, chromosome 8 at 45,029,456 bp
  • T to C, chromosome 8 at 70,243,225 bp
  • T to A, chromosome 8 at 92,360,671 bp
  • A to G, chromosome 8 at 106,202,333 bp
  • G to T, chromosome 8 at 114,706,341 bp
  • A to G, chromosome 9 at 15,341,474 bp
  • T to A, chromosome 9 at 35,272,520 bp
  • T to C, chromosome 9 at 38,349,749 bp
  • A to T, chromosome 9 at 44,353,924 bp
  • A to C, chromosome 9 at 58,020,834 bp
  • A to G, chromosome 10 at 23,985,256 bp
  • T to C, chromosome 10 at 56,088,337 bp
  • A to G, chromosome 10 at 74,379,357 bp
  • G to A, chromosome 10 at 75,175,335 bp
  • A to T, chromosome 10 at 116,972,592 bp
  • C to T, chromosome 11 at 5,281,776 bp
  • A to G, chromosome 11 at 46,756,137 bp
  • A to T, chromosome 11 at 48,837,686 bp
  • A to G, chromosome 11 at 58,220,187 bp
  • G to T, chromosome 11 at 69,778,964 bp
  • A to T, chromosome 11 at 70,251,587 bp
  • T to C, chromosome 11 at 74,288,853 bp
  • T to C, chromosome 11 at 103,615,896 bp
  • T to C, chromosome 12 at 25,095,724 bp
  • T to A, chromosome 12 at 55,377,217 bp
  • T to C, chromosome 12 at 75,929,417 bp
  • T to A, chromosome 12 at 88,321,432 bp
  • C to T, chromosome 13 at 14,637,780 bp
  • A to T, chromosome 13 at 21,396,291 bp
  • T to A, chromosome 13 at 49,672,614 bp
  • T to C, chromosome 13 at 56,735,985 bp
  • A to G, chromosome 13 at 75,968,117 bp
  • C to G, chromosome 14 at 20,324,561 bp
  • A to T, chromosome 14 at 21,423,548 bp
  • T to A, chromosome 14 at 29,993,073 bp
  • T to C, chromosome 14 at 52,217,938 bp
  • G to T, chromosome 14 at 54,965,394 bp
  • T to C, chromosome 14 at 62,587,537 bp
  • G to A, chromosome 14 at 70,203,303 bp
  • T to C, chromosome 14 at 118,957,964 bp
  • T to A, chromosome 15 at 9,182,406 bp
  • C to T, chromosome 15 at 12,834,496 bp
  • A to T, chromosome 15 at 39,292,182 bp
  • T to A, chromosome 15 at 97,384,347 bp
  • C to T, chromosome 16 at 4,523,028 bp
  • T to C, chromosome 16 at 18,302,690 bp
  • T to C, chromosome 17 at 12,433,895 bp
  • T to A, chromosome 17 at 24,620,766 bp
  • C to T, chromosome 17 at 32,011,175 bp
  • T to C, chromosome 17 at 38,336,020 bp
  • T to C, chromosome 17 at 56,109,567 bp
  • G to T, chromosome 18 at 56,575,492 bp
  • T to C, chromosome 19 at 6,492,871 bp
  • T to C, chromosome 19 at 6,855,980 bp
  • C to A, chromosome 19 at 9,009,347 bp
  • T to G, chromosome 19 at 24,114,100 bp
  • T to A, chromosome 19 at 29,121,767 bp
  • A to G, chromosome 19 at 47,364,638 bp
  • C to G, chromosome 19 at 47,654,220 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5988 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
044168-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.