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Strain Name:
C57BL/6J-MtgxR5991Btlr/Mmmh
Stock Number:
044171-MU
Citation ID:
RRID:MMRRC_044171-MU
Other Names:
R5991 (G1)
Major Collection:

Strain Information

Col1a1
Name: collagen, type I, alpha 1
Synonyms: Col1a-1, Mov-13, Cola1, Cola-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12842
HGNC: HGNC:2197
Homologene: 73874
Plin3
Name: perilipin 3
Synonyms: 1300012C15Rik, Tip47, M6prbp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66905
VEGA: 17
Homologene: 4247
Zmym6
Name: zinc finger, MYM-type 6
Synonyms: D4Wsu24e, 9330177P20Rik, Zfp258
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100177
Homologene: 5186
Tead3
Name: TEA domain family member 3
Synonyms: ETFR-1, TEAD-3, DTEF-1, Tcf13r2, TEF-5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21678
Homologene: 81821
Cnot7
Name: CCR4-NOT transcription complex, subunit 7
Synonyms: Caf1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18983
Homologene: 49011
Ltbp4
Name: latent transforming growth factor beta binding protein 4
Synonyms: 2310046A13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108075
HGNC: HGNC:6717
Homologene: 2645
Dnttip1
Name: deoxynucleotidyltransferase, terminal, interacting protein 1
Synonyms: 6430706C13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76233
Homologene: 14189
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 37,915,719 bp
  • T to A, chromosome 1 at 44,180,830 bp
  • T to C, chromosome 2 at 21,368,508 bp
  • A to G, chromosome 2 at 35,006,105 bp
  • A to T, chromosome 2 at 62,518,521 bp
  • C to T, chromosome 2 at 82,990,468 bp
  • A to G, chromosome 2 at 85,042,296 bp
  • A to G, chromosome 2 at 86,046,910 bp
  • C to T, chromosome 2 at 89,054,782 bp
  • T to A, chromosome 2 at 90,138,234 bp
  • C to T, chromosome 2 at 104,437,983 bp
  • C to T, chromosome 2 at 112,092,615 bp
  • A to T, chromosome 2 at 164,754,180 bp
  • G to T, chromosome 3 at 15,320,758 bp
  • T to A, chromosome 3 at 96,168,589 bp
  • G to A, chromosome 4 at 56,916,733 bp
  • C to A, chromosome 4 at 88,401,019 bp
  • T to C, chromosome 4 at 120,522,620 bp
  • T to A, chromosome 4 at 127,108,473 bp
  • A to G, chromosome 5 at 31,534,069 bp
  • A to G, chromosome 5 at 38,227,865 bp
  • A to T, chromosome 5 at 41,816,863 bp
  • G to C, chromosome 6 at 57,159,655 bp
  • A to G, chromosome 6 at 126,880,225 bp
  • A to G, chromosome 7 at 12,193,496 bp
  • A to G, chromosome 7 at 27,309,316 bp
  • G to T, chromosome 7 at 29,104,610 bp
  • T to A, chromosome 7 at 80,283,414 bp
  • T to A, chromosome 7 at 139,990,287 bp
  • A to C, chromosome 8 at 40,495,655 bp
  • T to C, chromosome 8 at 46,209,336 bp
  • G to T, chromosome 8 at 94,098,286 bp
  • A to G, chromosome 8 at 105,691,391 bp
  • G to A, chromosome 8 at 111,050,400 bp
  • G to A, chromosome 9 at 72,619,538 bp
  • A to T, chromosome 10 at 5,604,426 bp
  • A to G, chromosome 11 at 46,843,203 bp
  • T to C, chromosome 11 at 94,937,919 bp
  • T to C, chromosome 11 at 100,379,569 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • T to C, chromosome 11 at 107,171,145 bp
  • G to A, chromosome 11 at 113,943,254 bp
  • T to A, chromosome 13 at 33,259,807 bp
  • A to G, chromosome 15 at 81,648,466 bp
  • T to C, chromosome 16 at 19,514,757 bp
  • G to A, chromosome 16 at 35,841,457 bp
  • T to A, chromosome 17 at 6,289,037 bp
  • G to A, chromosome 17 at 24,209,069 bp
  • A to T, chromosome 17 at 28,334,378 bp
  • G to T, chromosome 17 at 45,659,352 bp
  • T to C, chromosome 17 at 56,286,576 bp
  • T to C, chromosome 18 at 37,301,508 bp
  • T to C, chromosome 18 at 53,721,798 bp
  • T to A, chromosome 18 at 62,657,885 bp
  • C to T, chromosome 19 at 29,454,467 bp
  • T to A, chromosome 19 at 54,003,049 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5991 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044171-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.