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Strain Name:
C57BL/6J-MtgxR5995Btlr/Mmmh
Stock Number:
044174-MU
Citation ID:
RRID:MMRRC_044174-MU
Other Names:
R5995 (G1)
Major Collection:

Strain Information

Nfkbil1
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor like 1
Synonyms: Def-7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18038
HGNC: HGNC:7800
Homologene: 3671
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain II-B, NMHC-B, SMemb, nonmuscle myosin heavy chain IIB, 9330167F11Rik, 5730504C04Rik, NMHC II-B, Myhn2, Myosin IIB, Myhn-2, myosin IIB, Fltn, Fltn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Supt20
Name: SPT20 SAGA complex component
Synonyms: p38 interacting protein, p38IP, D3Ertd300e, Fam48a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56790
Homologene: 134155
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: 5330419I02Rik, D530019K20Rik, G1-403-16, Wdr9, repro5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 93871
Homologene: 23130
Cyp7a1
Name: cytochrome P450, family 7, subfamily a, polypeptide 1
Synonyms: cholesterol 7 alpha hydroxylase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13122
HGNC: HGNC:2651
Homologene: 30987
Abcb8
Name: ATP-binding cassette, sub-family B member 8
Synonyms: 4833412N02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74610
HGNC: HGNC:49
Homologene: 5203
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 33,878,439 bp
  • A to T, chromosome 1 at 53,620,670 bp
  • A to G, chromosome 2 at 76,761,292 bp
  • T to C, chromosome 2 at 87,135,856 bp
  • A to G, chromosome 2 at 102,861,670 bp
  • T to C, chromosome 3 at 54,709,053 bp
  • C to T, chromosome 4 at 6,272,371 bp
  • T to A, chromosome 4 at 113,893,832 bp
  • C to A, chromosome 4 at 130,017,103 bp
  • A to T, chromosome 4 at 145,701,909 bp
  • T to C, chromosome 4 at 150,168,340 bp
  • T to A, chromosome 5 at 3,607,704 bp
  • C to T, chromosome 5 at 24,400,139 bp
  • A to G, chromosome 5 at 36,486,374 bp
  • T to A, chromosome 5 at 109,047,055 bp
  • T to C, chromosome 5 at 137,378,809 bp
  • T to A, chromosome 6 at 52,170,283 bp
  • A to G, chromosome 6 at 54,944,554 bp
  • T to A, chromosome 7 at 17,053,769 bp
  • T to G, chromosome 7 at 25,097,444 bp
  • A to C, chromosome 7 at 126,589,363 bp
  • T to A, chromosome 8 at 71,623,110 bp
  • T to A, chromosome 8 at 104,850,901 bp
  • A to G, chromosome 9 at 39,772,692 bp
  • G to A, chromosome 9 at 40,994,540 bp
  • T to C, chromosome 9 at 53,395,895 bp
  • A to T, chromosome 9 at 78,700,279 bp
  • C to G, chromosome 9 at 103,364,003 bp
  • A to G, chromosome 9 at 122,157,222 bp
  • A to C, chromosome 10 at 6,832,520 bp
  • A to T, chromosome 10 at 45,670,391 bp
  • T to C, chromosome 11 at 48,915,713 bp
  • A to T, chromosome 11 at 57,289,285 bp
  • T to C, chromosome 11 at 68,814,983 bp
  • A to T, chromosome 11 at 71,181,746 bp
  • T to C, chromosome 11 at 73,589,250 bp
  • T to C, chromosome 12 at 69,250,774 bp
  • A to G, chromosome 13 at 40,280,422 bp
  • A to G, chromosome 13 at 81,466,259 bp
  • T to G, chromosome 14 at 50,892,651 bp
  • C to T, chromosome 14 at 51,364,348 bp
  • A to T, chromosome 15 at 76,185,905 bp
  • A to T, chromosome 16 at 96,064,787 bp
  • T to C, chromosome 17 at 18,168,951 bp
  • G to T, chromosome 17 at 35,220,798 bp
  • A to G, chromosome 17 at 36,041,485 bp
  • C to A, chromosome 17 at 37,263,648 bp
  • C to A, chromosome 18 at 13,717,624 bp
  • T to A, chromosome 18 at 37,821,060 bp
  • T to A, chromosome 18 at 44,886,253 bp
  • T to A, chromosome 18 at 61,990,010 bp
  • G to T, chromosome 18 at 64,341,548 bp
  • T to C, chromosome 18 at 89,421,018 bp
  • A to T, chromosome 19 at 11,870,862 bp
  • A to C, chromosome 19 at 12,890,597 bp
  • G to A, chromosome 19 at 53,851,267 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5995 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044174-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.