Strain Name:
C57BL/6J-MtgxR6000Btlr
Stock Number:
044179-MU
Citation ID:
RRID:MMRRC_044179-MU
Other Names:
R6000 (G1)
Major Collection:

Gene Information

Gene Symbol: Dst [MGI:104627] (Mus musculus (mouse))
Name: dystonin
Synonyms: ah, 2310001O04Rik, bullous pemphigoid antigen 1, A830042E19Rik, Bpag, Macf2, athetoid, Bpag1, nmf339, nmf203, BPAG1, bullous pemphigoid antigen 1, BPAG1-n
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Ep400 [MGI:1276124] (Mus musculus (mouse))
Name: E1A binding protein p400
Synonyms: 1700020J09Rik, mDomino, p400
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Prkdc [MGI:104779] (Mus musculus (mouse))
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNAPDcs, DNA-PK, slip, DOXNPH, XRCC7, DNA-PKcs, dxnph
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Sgsm1 [MGI:107320] (Mus musculus (mouse))
Name: small G protein signaling modulator 1
Synonyms: Rutbc2, 2410098H20Rik, D5Bwg1524e
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Ylpm1 [MGI:1926195] (Mus musculus (mouse))
Name: YLP motif containing 1
Synonyms: ZAP, A930013E17Rik, Zap3
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Igll1 [MGI:96529] (Mus musculus (mouse))
Name: immunoglobulin lambda-like polypeptide 1
Synonyms: Igl-5, Lambda 5, Igll
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Alox12b [MGI:1274782] (Mus musculus (mouse))
Name: arachidonate 12-lipoxygenase, 12R type
Synonyms: 12R-LOX, e-LOX2, Aloxe2
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,212,223 bp
  • C to T, chromosome 1 at 53,061,669 bp
  • A to T, chromosome 1 at 58,911,812 bp
  • G to A, chromosome 1 at 59,289,659 bp
  • A to T, chromosome 1 at 84,383,929 bp
  • G to T, chromosome 1 at 188,267,026 bp
  • A to T, chromosome 2 at 76,017,860 bp
  • C to A, chromosome 2 at 76,745,172 bp
  • A to T, chromosome 2 at 76,885,151 bp
  • C to A, chromosome 2 at 88,729,231 bp
  • G to T, chromosome 2 at 119,374,508 bp
  • A to T, chromosome 3 at 40,654,148 bp
  • A to C, chromosome 3 at 49,754,464 bp
  • A to G, chromosome 3 at 121,156,513 bp
  • T to A, chromosome 4 at 43,518,301 bp
  • A to T, chromosome 4 at 118,710,244 bp
  • A to T, chromosome 4 at 136,684,030 bp
  • A to G, chromosome 5 at 90,603,877 bp
  • G to T, chromosome 5 at 99,941,367 bp
  • G to A, chromosome 5 at 102,966,475 bp
  • G to A, chromosome 5 at 102,966,476 bp
  • A to G, chromosome 5 at 109,676,864 bp
  • A to G, chromosome 5 at 110,683,201 bp
  • A to T, chromosome 5 at 113,286,838 bp
  • A to G, chromosome 5 at 123,254,372 bp
  • A to G, chromosome 5 at 144,211,421 bp
  • A to T, chromosome 6 at 48,907,639 bp
  • T to C, chromosome 6 at 121,021,320 bp
  • T to C, chromosome 6 at 121,765,079 bp
  • T to G, chromosome 6 at 128,784,157 bp
  • A to T, chromosome 7 at 18,482,692 bp
  • A to T, chromosome 7 at 19,347,161 bp
  • A to C, chromosome 7 at 25,271,998 bp
  • T to C, chromosome 7 at 81,361,680 bp
  • T to A, chromosome 7 at 86,725,934 bp
  • T to C, chromosome 7 at 109,029,650 bp
  • A to G, chromosome 8 at 122,891,195 bp
  • C to T, chromosome 9 at 37,315,764 bp
  • T to A, chromosome 9 at 61,374,014 bp
  • A to T, chromosome 9 at 108,301,485 bp
  • T to C, chromosome 10 at 39,158,300 bp
  • C to T, chromosome 10 at 41,561,163 bp
  • T to A, chromosome 10 at 80,638,944 bp
  • T to A, chromosome 10 at 100,541,787 bp
  • A to G, chromosome 11 at 8,950,427 bp
  • A to T, chromosome 11 at 12,369,684 bp
  • CTGCT to CTGCTTGCT, chromosome 11 at 65,032,084 bp
  • A to G, chromosome 11 at 69,169,568 bp
  • A to G, chromosome 11 at 69,403,598 bp
  • C to A, chromosome 12 at 26,447,151 bp
  • C to A, chromosome 12 at 40,216,244 bp
  • A to G, chromosome 12 at 51,445,674 bp
  • C to A, chromosome 12 at 84,997,256 bp
  • C to A, chromosome 12 at 87,057,197 bp
  • A to T, chromosome 14 at 62,419,050 bp
  • A to G, chromosome 14 at 70,567,833 bp
  • G to A, chromosome 15 at 98,313,436 bp
  • G to A, chromosome 15 at 99,426,885 bp
  • T to C, chromosome 15 at 103,478,815 bp
  • A to T, chromosome 16 at 15,829,697 bp
  • C to A, chromosome 16 at 16,863,941 bp
  • T to A, chromosome 16 at 19,700,948 bp
  • T to C, chromosome 16 at 88,759,539 bp
  • A to T, chromosome 17 at 25,036,960 bp
  • G to T, chromosome 17 at 33,939,615 bp
  • A to T, chromosome 19 at 9,013,111 bp
  • A to G, chromosome 19 at 39,307,606 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6000 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044179-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.