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Strain Name:
C57BL/6J-MtgxR6027Btlr/Mmmh
Stock Number:
044199-MU
Citation ID:
RRID:MMRRC_044199-MU
Other Names:
R6027 (G1)
Major Collection:

Strain Information

Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: Fadk, FAK, FRNK
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Hmox1
Name: heme oxygenase 1
Synonyms: Hsp32, HO-1, heme oxygenase 1, D8Wsu38e, Hmox, HO1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15368
HGNC: HGNC:5013
Homologene: 31075
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Fkbp3
Name: FK506 binding protein 3
Synonyms: FKBP25, 25kDa
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 30795
VEGA: 12
HGNC: HGNC:3719
Homologene: 1525
Asah2
Name: N-acylsphingosine amidohydrolase 2
Synonyms: neutral/alkaline ceramidase
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54447
VEGA: 19
Homologene: 10310
Armc9
Name: armadillo repeat containing 9
Synonyms: 5730415N24Rik, 3830422A13Rik, 4831423D23Rik, 4930438O05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78795
Homologene: 11847
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,837,076 bp
  • T to C, chromosome 1 at 85,577,318 bp
  • G to C, chromosome 1 at 86,244,667 bp
  • G to A, chromosome 1 at 86,387,331 bp
  • A to T, chromosome 1 at 93,025,643 bp
  • T to A, chromosome 1 at 95,653,674 bp
  • T to C, chromosome 1 at 136,483,059 bp
  • T to G, chromosome 1 at 139,463,056 bp
  • A to G, chromosome 1 at 150,802,895 bp
  • T to G, chromosome 2 at 86,657,804 bp
  • T to A, chromosome 2 at 163,451,611 bp
  • A to G, chromosome 3 at 36,222,038 bp
  • T to C, chromosome 3 at 88,985,019 bp
  • G to T, chromosome 3 at 125,713,376 bp
  • T to C, chromosome 3 at 126,997,879 bp
  • A to G, chromosome 4 at 43,835,842 bp
  • T to C, chromosome 4 at 113,096,564 bp
  • G to A, chromosome 4 at 128,559,946 bp
  • C to T, chromosome 5 at 63,808,220 bp
  • A to G, chromosome 5 at 114,165,600 bp
  • A to C, chromosome 5 at 148,333,964 bp
  • T to C, chromosome 7 at 3,824,639 bp
  • T to G, chromosome 7 at 100,938,508 bp
  • T to C, chromosome 7 at 122,133,341 bp
  • T to A, chromosome 7 at 126,487,748 bp
  • C to A, chromosome 8 at 75,096,871 bp
  • C to T, chromosome 8 at 85,096,752 bp
  • C to A, chromosome 8 at 95,372,176 bp
  • A to G, chromosome 8 at 117,158,295 bp
  • A to T, chromosome 9 at 14,357,597 bp
  • ATTGCTGTTT to ATTGCTGTTTGCTGTTT, chromosome 9 at 38,026,540 bp
  • A to T, chromosome 9 at 44,819,290 bp
  • C to T, chromosome 9 at 79,656,578 bp
  • A to C, chromosome 9 at 90,191,025 bp
  • G to T, chromosome 10 at 52,163,968 bp
  • A to T, chromosome 10 at 79,887,018 bp
  • A to G, chromosome 10 at 86,048,086 bp
  • A to G, chromosome 10 at 88,433,225 bp
  • C to A, chromosome 11 at 8,916,272 bp
  • T to C, chromosome 11 at 29,547,808 bp
  • C to T, chromosome 11 at 58,978,463 bp
  • T to A, chromosome 11 at 78,483,558 bp
  • T to C, chromosome 11 at 84,398,177 bp
  • T to C, chromosome 11 at 107,074,945 bp
  • T to A, chromosome 12 at 11,306,178 bp
  • T to C, chromosome 12 at 40,077,516 bp
  • G to T, chromosome 12 at 65,073,918 bp
  • T to A, chromosome 12 at 104,031,077 bp
  • T to A, chromosome 13 at 34,910,994 bp
  • A to T, chromosome 13 at 34,985,947 bp
  • G to A, chromosome 13 at 104,479,535 bp
  • T to C, chromosome 14 at 12,220,613 bp
  • A to T, chromosome 14 at 54,970,802 bp
  • A to G, chromosome 14 at 55,486,123 bp
  • G to A, chromosome 14 at 56,629,158 bp
  • T to A, chromosome 15 at 73,229,913 bp
  • A to G, chromosome 15 at 83,967,721 bp
  • A to G, chromosome 16 at 97,744,074 bp
  • A to G, chromosome 17 at 8,964,677 bp
  • C to T, chromosome 17 at 33,818,114 bp
  • T to A, chromosome 18 at 44,860,436 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • T to C, chromosome 19 at 32,044,951 bp
  • T to A, chromosome 19 at 46,582,852 bp
  • A to G, chromosome 19 at 58,946,830 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6027 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044199-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.