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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR6029Btlr/Mmmh
Stock Number:
044201-MU
Citation ID:
RRID:MMRRC_044201-MU
Other Names:
R6029 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Sh3gl2
Name: SH3-domain GRB2-like 2
Synonyms: EEN-B1, endophilin I, Sh3d2a, 9530001L19Rik, B930049H17Rik, endophilin A1, EEN1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20404
Homologene: 20652

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
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Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Oxr1
Name: oxidation resistance 1
Synonyms: C7B, C7, 2210416C20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170719
VEGA: 15
Homologene: 24993

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Mapkapk3
Name: mitogen-activated protein kinase-activated protein kinase 3
Synonyms: MK3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102626
HGNC: HGNC:6888
Homologene: 55836

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 80,536,946 bp
  • G to A, chromosome 1 at 87,187,557 bp
  • T to C, chromosome 1 at 118,200,260 bp
  • T to G, chromosome 1 at 126,025,786 bp
  • T to G, chromosome 1 at 139,480,990 bp
  • C to A, chromosome 1 at 150,632,437 bp
  • T to C, chromosome 1 at 175,978,746 bp
  • T to C, chromosome 1 at 180,111,787 bp
  • A to G, chromosome 1 at 194,794,427 bp
  • A to T, chromosome 1 at 194,799,575 bp
  • T to A, chromosome 2 at 19,394,311 bp
  • G to A, chromosome 2 at 68,694,026 bp
  • T to C, chromosome 2 at 88,910,036 bp
  • T to A, chromosome 2 at 111,478,965 bp
  • A to T, chromosome 2 at 125,563,632 bp
  • G to A, chromosome 2 at 142,318,447 bp
  • G to A, chromosome 2 at 153,259,793 bp
  • T to A, chromosome 2 at 155,179,089 bp
  • T to C, chromosome 2 at 158,755,217 bp
  • T to C, chromosome 3 at 8,923,745 bp
  • T to C, chromosome 3 at 37,642,848 bp
  • G to A, chromosome 3 at 72,928,308 bp
  • T to A, chromosome 3 at 81,961,440 bp
  • T to C, chromosome 3 at 88,985,019 bp
  • G to T, chromosome 4 at 46,506,998 bp
  • G to A, chromosome 4 at 85,381,414 bp
  • C to A, chromosome 4 at 123,507,333 bp
  • A to G, chromosome 4 at 129,428,493 bp
  • A to G, chromosome 4 at 131,771,293 bp
  • A to G, chromosome 4 at 155,889,463 bp
  • A to T, chromosome 5 at 11,920,713 bp
  • C to T, chromosome 5 at 31,054,983 bp
  • C to A, chromosome 5 at 31,526,987 bp
  • T to C, chromosome 5 at 43,765,404 bp
  • G to T, chromosome 5 at 45,460,186 bp
  • A to G, chromosome 5 at 88,627,255 bp
  • A to T, chromosome 5 at 122,879,587 bp
  • T to C, chromosome 5 at 144,817,679 bp
  • T to C, chromosome 5 at 144,825,914 bp
  • T to G, chromosome 6 at 57,390,466 bp
  • T to C, chromosome 6 at 136,911,611 bp
  • A to T, chromosome 7 at 35,485,230 bp
  • A to G, chromosome 7 at 42,708,130 bp
  • G to T, chromosome 7 at 44,462,330 bp
  • C to T, chromosome 7 at 44,965,124 bp
  • A to G, chromosome 7 at 73,960,710 bp
  • T to C, chromosome 7 at 130,819,072 bp
  • A to T, chromosome 7 at 140,538,876 bp
  • A to T, chromosome 8 at 40,472,927 bp
  • A to G, chromosome 9 at 7,157,646 bp
  • G to A, chromosome 9 at 7,639,301 bp
  • ATTGCTGTTT to ATTGCTGTTTGCTGTTT, chromosome 9 at 38,026,540 bp
  • G to A, chromosome 9 at 39,702,400 bp
  • T to A, chromosome 9 at 62,841,514 bp
  • T to A, chromosome 9 at 69,364,452 bp
  • A to T, chromosome 9 at 86,825,080 bp
  • T to C, chromosome 9 at 97,456,581 bp
  • A to T, chromosome 9 at 103,344,953 bp
  • G to T, chromosome 9 at 107,289,226 bp
  • A to G, chromosome 9 at 107,583,554 bp
  • A to T, chromosome 9 at 107,652,175 bp
  • A to T, chromosome 9 at 108,513,690 bp
  • A to T, chromosome 9 at 109,329,425 bp
  • A to G, chromosome 10 at 22,148,933 bp
  • T to C, chromosome 10 at 61,747,762 bp
  • G to A, chromosome 10 at 93,846,632 bp
  • G to T, chromosome 11 at 4,784,566 bp
  • G to T, chromosome 11 at 33,623,477 bp
  • A to T, chromosome 11 at 49,564,601 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • A to G, chromosome 11 at 82,913,327 bp
  • G to T, chromosome 11 at 85,025,582 bp
  • G to A, chromosome 11 at 100,012,463 bp
  • G to T, chromosome 11 at 120,820,909 bp
  • A to G, chromosome 12 at 8,016,243 bp
  • G to A, chromosome 12 at 28,679,319 bp
  • T to A, chromosome 13 at 12,574,833 bp
  • A to T, chromosome 14 at 34,739,247 bp
  • T to G, chromosome 15 at 41,825,901 bp
  • T to A, chromosome 15 at 100,807,339 bp
  • C to T, chromosome 16 at 25,868,214 bp
  • T to A, chromosome 16 at 31,793,570 bp
  • T to C, chromosome 16 at 46,436,400 bp
  • A to G, chromosome 17 at 17,831,196 bp
  • T to A, chromosome 17 at 19,794,216 bp
  • A to G, chromosome 17 at 21,662,485 bp
  • C to T, chromosome 17 at 27,098,171 bp
  • T to C, chromosome 17 at 56,396,576 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • A to G, chromosome 19 at 3,802,104 bp
  • A to G, chromosome 19 at 29,754,967 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6029 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
044201-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.