Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6031Btlr/Mmmh
Stock Number:
044203-MU
Citation ID:
RRID:MMRRC_044203-MU
Other Names:
R6031 (G1)
Major Collection:

Strain Information

Atg7
Name: autophagy related 7
Synonyms: 1810013K23Rik, Apg7l
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74244
Homologene: 4662
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
Numa1
Name: nuclear mitotic apparatus protein 1
Synonyms: 6720401E04Rik, NuMA
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101706
HGNC: HGNC:8059
Homologene: 38150
Ndc80
Name: NDC80 kinetochore complex component
Synonyms: HEC1, 2610020P18Rik, Kntc2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67052
VEGA: 17
Homologene: 38141
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 88,354,469 bp
  • A to T, chromosome 1 at 136,280,438 bp
  • A to G, chromosome 1 at 191,340,840 bp
  • A to C, chromosome 2 at 25,243,764 bp
  • G to A, chromosome 2 at 28,613,808 bp
  • A to G, chromosome 2 at 30,824,195 bp
  • A to T, chromosome 2 at 76,830,597 bp
  • T to A, chromosome 2 at 89,406,972 bp
  • G to T, chromosome 2 at 118,789,794 bp
  • A to G, chromosome 2 at 128,967,812 bp
  • T to C, chromosome 3 at 85,613,511 bp
  • T to A, chromosome 3 at 89,218,094 bp
  • A to G, chromosome 3 at 129,926,389 bp
  • A to G, chromosome 3 at 145,139,863 bp
  • A to G, chromosome 4 at 25,278,038 bp
  • G to A, chromosome 4 at 41,757,202 bp
  • T to C, chromosome 4 at 74,187,451 bp
  • A to T, chromosome 4 at 118,830,391 bp
  • A to G, chromosome 4 at 134,279,041 bp
  • A to T, chromosome 4 at 141,034,357 bp
  • A to C, chromosome 4 at 145,168,509 bp
  • T to C, chromosome 5 at 122,129,736 bp
  • A to C, chromosome 6 at 34,312,674 bp
  • A to T, chromosome 6 at 37,334,434 bp
  • A to G, chromosome 6 at 57,071,173 bp
  • A to T, chromosome 6 at 85,622,955 bp
  • A to G, chromosome 6 at 86,098,673 bp
  • C to A, chromosome 6 at 87,086,320 bp
  • T to A, chromosome 6 at 114,671,233 bp
  • T to C, chromosome 6 at 125,133,566 bp
  • A to T, chromosome 7 at 4,663,466 bp
  • G to T, chromosome 7 at 24,276,300 bp
  • T to A, chromosome 7 at 102,012,012 bp
  • C to T, chromosome 7 at 106,885,927 bp
  • A to T, chromosome 7 at 140,162,809 bp
  • A to T, chromosome 7 at 142,376,714 bp
  • A to G, chromosome 8 at 45,398,528 bp
  • T to A, chromosome 9 at 15,988,492 bp
  • T to C, chromosome 9 at 21,275,269 bp
  • A to C, chromosome 9 at 29,009,375 bp
  • G to A, chromosome 9 at 35,561,184 bp
  • C to T, chromosome 10 at 24,903,748 bp
  • C to T, chromosome 10 at 50,842,183 bp
  • A to G, chromosome 10 at 129,174,355 bp
  • G to T, chromosome 11 at 51,826,586 bp
  • A to G, chromosome 11 at 57,217,782 bp
  • A to T, chromosome 11 at 75,447,869 bp
  • T to C, chromosome 11 at 76,790,888 bp
  • A to T, chromosome 11 at 97,476,139 bp
  • A to G, chromosome 12 at 3,967,106 bp
  • T to C, chromosome 12 at 84,417,990 bp
  • T to C, chromosome 13 at 49,705,831 bp
  • C to T, chromosome 13 at 100,684,369 bp
  • G to A, chromosome 14 at 55,632,139 bp
  • A to T, chromosome 14 at 119,070,826 bp
  • T to A, chromosome 15 at 35,471,968 bp
  • A to T, chromosome 15 at 100,983,984 bp
  • T to A, chromosome 16 at 32,256,718 bp
  • T to C, chromosome 16 at 59,215,933 bp
  • T to A, chromosome 16 at 65,839,481 bp
  • T to C, chromosome 17 at 8,995,252 bp
  • C to T, chromosome 17 at 21,993,376 bp
  • T to C, chromosome 17 at 71,511,488 bp
  • A to G, chromosome 17 at 75,539,432 bp
  • T to C, chromosome 17 at 90,588,790 bp
  • A to T, chromosome 18 at 37,497,723 bp
  • T to A, chromosome 19 at 3,371,556 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6031 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044203-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.