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Strain Name:
C57BL/6J-MtgxR6052Btlr/Mmmh
Stock Number:
044220-MU
Citation ID:
RRID:MMRRC_044220-MU
Other Names:
R6052 (G1)
Major Collection:

Strain Information

Wnt3
Name: wingless-type MMTV integration site family, member 3
Synonyms: Wnt-3, Int-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22415
Homologene: 22527
Rest
Name: RE1-silencing transcription factor
Synonyms: 2610008J04Rik, NRSF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19712
HGNC: HGNC:9966
Homologene: 4099
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Nae1
Name: NEDD8 activating enzyme E1 subunit 1
Synonyms: Appbp1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234664
HGNC: HGNC:621
Homologene: 68370
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 14,283,150 bp
  • T to C, chromosome 1 at 45,345,013 bp
  • T to A, chromosome 1 at 58,254,318 bp
  • T to C, chromosome 1 at 84,912,709 bp
  • T to C, chromosome 1 at 181,666,487 bp
  • T to C, chromosome 1 at 183,424,057 bp
  • A to C, chromosome 2 at 32,212,285 bp
  • A to G, chromosome 2 at 32,779,851 bp
  • T to C, chromosome 2 at 53,159,281 bp
  • T to G, chromosome 2 at 122,097,356 bp
  • T to C, chromosome 2 at 154,510,581 bp
  • T to C, chromosome 2 at 163,470,116 bp
  • T to A, chromosome 3 at 15,990,753 bp
  • T to C, chromosome 3 at 64,117,361 bp
  • G to C, chromosome 4 at 43,555,052 bp
  • C to A, chromosome 4 at 43,568,330 bp
  • T to A, chromosome 4 at 116,151,602 bp
  • C to T, chromosome 4 at 116,599,948 bp
  • T to A, chromosome 4 at 117,564,628 bp
  • T to A, chromosome 4 at 143,655,652 bp
  • T to G, chromosome 5 at 77,281,180 bp
  • T to C, chromosome 5 at 90,253,832 bp
  • T to A, chromosome 5 at 96,764,866 bp
  • T to A, chromosome 5 at 124,886,186 bp
  • T to C, chromosome 6 at 21,772,638 bp
  • T to A, chromosome 6 at 42,486,654 bp
  • C to T, chromosome 6 at 70,143,689 bp
  • A to T, chromosome 7 at 30,958,617 bp
  • T to A, chromosome 7 at 44,829,040 bp
  • G to A, chromosome 7 at 45,720,130 bp
  • G to A, chromosome 7 at 101,404,033 bp
  • A to T, chromosome 7 at 106,926,689 bp
  • A to G, chromosome 7 at 108,523,738 bp
  • A to G, chromosome 7 at 110,441,534 bp
  • T to C, chromosome 8 at 41,200,511 bp
  • C to T, chromosome 8 at 70,291,426 bp
  • G to A, chromosome 8 at 71,914,505 bp
  • T to C, chromosome 8 at 104,534,544 bp
  • T to C, chromosome 8 at 105,254,598 bp
  • T to A, chromosome 8 at 105,990,638 bp
  • C to A, chromosome 8 at 111,078,816 bp
  • G to A, chromosome 8 at 122,506,269 bp
  • T to A, chromosome 9 at 15,922,679 bp
  • A to G, chromosome 9 at 64,886,945 bp
  • T to A, chromosome 9 at 103,190,216 bp
  • T to A, chromosome 9 at 108,487,612 bp
  • T to C, chromosome 9 at 115,067,315 bp
  • A to G, chromosome 9 at 123,185,331 bp
  • T to A, chromosome 10 at 52,163,903 bp
  • T to A, chromosome 10 at 94,943,773 bp
  • C to A, chromosome 10 at 129,333,202 bp
  • A to G, chromosome 11 at 16,911,554 bp
  • G to T, chromosome 11 at 32,255,453 bp
  • T to C, chromosome 11 at 50,297,119 bp
  • A to G, chromosome 11 at 65,196,256 bp
  • A to G, chromosome 11 at 78,018,218 bp
  • C to A, chromosome 11 at 103,808,174 bp
  • G to A, chromosome 12 at 26,450,578 bp
  • A to T, chromosome 12 at 50,366,300 bp
  • T to C, chromosome 12 at 110,918,891 bp
  • A to T, chromosome 13 at 17,721,215 bp
  • G to A, chromosome 13 at 67,293,951 bp
  • A to G, chromosome 13 at 119,705,459 bp
  • T to C, chromosome 14 at 70,683,719 bp
  • C to T, chromosome 14 at 93,885,846 bp
  • A to G, chromosome 15 at 81,009,263 bp
  • A to G, chromosome 15 at 101,922,759 bp
  • G to A, chromosome 16 at 4,613,739 bp
  • T to A, chromosome 16 at 34,360,885 bp
  • C to A, chromosome 16 at 43,821,283 bp
  • C to A, chromosome 16 at 63,603,604 bp
  • C to T, chromosome 16 at 90,762,383 bp
  • T to A, chromosome 17 at 6,280,615 bp
  • A to G, chromosome 17 at 24,318,191 bp
  • A to T, chromosome 17 at 25,248,470 bp
  • A to G, chromosome 17 at 34,027,455 bp
  • G to A, chromosome 17 at 34,214,709 bp
  • A to G, chromosome 18 at 84,014,069 bp
  • T to G, chromosome 19 at 5,697,402 bp
  • C to A, chromosome 19 at 13,654,507 bp
  • T to C, chromosome 19 at 40,720,484 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6052 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044220-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.