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Strain Name:
C57BL/6J-MtgxR6109Btlr/Mmmh
Stock Number:
044259-MU
Citation ID:
RRID:MMRRC_044259-MU
Other Names:
R6109 (G1)
Major Collection:

Strain Information

Mitf
Name: melanogenesis associated transcription factor
Synonyms: mi, wh, BCC2, bHLHe32, Gsfbcc2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17342
HGNC: HGNC:7105
Homologene: 4892
Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Pdk1
Name: pyruvate dehydrogenase kinase, isoenzyme 1
Synonyms: D530020C15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228026
HGNC: HGNC:8809
Homologene: 134437
Gk5
Name: glycerol kinase 5
Synonyms: G630067D24Rik, C330018K18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235533
Homologene: 15141
Wdr70
Name: WD repeat domain 70
Synonyms: 4833422F06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 545085
VEGA: 15
Homologene: 9972
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 36,498,479 bp
  • A to G, chromosome 1 at 78,463,270 bp
  • T to C, chromosome 1 at 79,810,671 bp
  • G to A, chromosome 1 at 104,994,014 bp
  • C to A, chromosome 1 at 175,637,908 bp
  • A to G, chromosome 2 at 25,779,296 bp
  • A to G, chromosome 2 at 71,883,506 bp
  • T to C, chromosome 3 at 90,542,074 bp
  • A to G, chromosome 3 at 92,933,687 bp
  • T to A, chromosome 3 at 108,100,135 bp
  • C to T, chromosome 3 at 109,664,365 bp
  • A to G, chromosome 3 at 153,941,943 bp
  • A to G, chromosome 4 at 139,417,364 bp
  • C to T, chromosome 5 at 113,357,541 bp
  • C to T, chromosome 5 at 122,344,297 bp
  • C to T, chromosome 5 at 125,055,846 bp
  • C to A, chromosome 5 at 138,820,095 bp
  • A to T, chromosome 6 at 41,688,862 bp
  • A to T, chromosome 6 at 43,305,169 bp
  • C to T, chromosome 6 at 97,996,468 bp
  • T to C, chromosome 7 at 24,668,313 bp
  • T to A, chromosome 7 at 81,493,592 bp
  • T to C, chromosome 7 at 103,565,139 bp
  • C to T, chromosome 7 at 127,194,815 bp
  • C to T, chromosome 7 at 137,206,226 bp
  • T to C, chromosome 8 at 16,199,860 bp
  • T to C, chromosome 9 at 18,655,359 bp
  • T to A, chromosome 9 at 32,260,111 bp
  • A to G, chromosome 9 at 39,060,196 bp
  • C to T, chromosome 9 at 56,259,283 bp
  • C to T, chromosome 9 at 65,299,952 bp
  • C to A, chromosome 9 at 96,140,610 bp
  • A to G, chromosome 10 at 36,986,389 bp
  • A to G, chromosome 10 at 50,649,247 bp
  • G to A, chromosome 10 at 129,801,820 bp
  • C to A, chromosome 10 at 129,801,821 bp
  • C to A, chromosome 11 at 5,628,941 bp
  • C to A, chromosome 11 at 58,920,304 bp
  • T to G, chromosome 11 at 95,578,057 bp
  • T to C, chromosome 11 at 100,087,965 bp
  • T to C, chromosome 11 at 119,231,978 bp
  • G to T, chromosome 13 at 21,381,139 bp
  • G to T, chromosome 13 at 59,473,746 bp
  • C to T, chromosome 13 at 68,590,218 bp
  • A to T, chromosome 13 at 77,126,680 bp
  • A to G, chromosome 13 at 100,427,182 bp
  • A to T, chromosome 15 at 8,079,154 bp
  • C to T, chromosome 15 at 10,377,422 bp
  • T to C, chromosome 15 at 76,373,716 bp
  • T to A, chromosome 16 at 33,069,111 bp
  • T to A, chromosome 16 at 44,953,682 bp
  • T to A, chromosome 17 at 23,607,129 bp
  • A to G, chromosome 17 at 24,813,019 bp
  • T to A, chromosome 17 at 32,797,374 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • A to T, chromosome 17 at 35,263,937 bp
  • T to A, chromosome 17 at 46,301,697 bp
  • T to C, chromosome 17 at 46,310,377 bp
  • G to T, chromosome 18 at 37,265,253 bp
  • A to T, chromosome 18 at 60,943,234 bp
  • A to T, chromosome 18 at 62,937,366 bp
  • T to C, chromosome 19 at 5,467,119 bp
  • T to C, chromosome 19 at 11,101,912 bp
  • T to C, chromosome Y at 921,501 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6109 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044259-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.