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Strain Name:
C57BL/6J-MtgxR6115Btlr/Mmmh
Stock Number:
044264-MU
Citation ID:
RRID:MMRRC_044264-MU
Other Names:
R6115 (G1)
Major Collection:

Strain Information

Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Tm9sf4
Name: transmembrane 9 superfamily member 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99237
Homologene: 21620
Sfpq
Name: splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
Synonyms: D4Ertd314e, 9030402K04Rik, 2810416M14Rik, 5730453G22Rik, 1110004P21Rik, REP1, PSF
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 71514
Homologene: 3714
Nsmce4a
Name: NSE4 homolog A, SMC5-SMC6 complex component
Synonyms: 2410003A14Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67872
Homologene: 9745
Prpf31
Name: pre-mRNA processing factor 31
Synonyms: 2810404O06Rik, PRP31, RP11, 1500019O16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68988
Homologene: 5980
Unc5b
Name: unc-5 netrin receptor B
Synonyms: 6330415E02Rik, Unc5h2, D10Bwg0792e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 107449
VEGA: 10
Homologene: 32538
Lrfn4
Name: leucine rich repeat and fibronectin type III domain containing 4
Synonyms: SALM3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225875
Homologene: 36415
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 127,677,448 bp
  • T to C, chromosome 1 at 130,719,063 bp
  • T to C, chromosome 2 at 33,569,106 bp
  • T to G, chromosome 2 at 35,013,038 bp
  • A to T, chromosome 2 at 36,568,951 bp
  • T to C, chromosome 2 at 63,979,310 bp
  • T to A, chromosome 2 at 66,563,629 bp
  • A to G, chromosome 2 at 111,329,213 bp
  • A to T, chromosome 2 at 111,664,642 bp
  • T to C, chromosome 2 at 153,182,489 bp
  • G to C, chromosome 2 at 178,348,245 bp
  • C to T, chromosome 3 at 36,182,593 bp
  • A to T, chromosome 3 at 133,132,613 bp
  • A to T, chromosome 4 at 127,021,348 bp
  • C to G, chromosome 5 at 63,897,974 bp
  • T to A, chromosome 5 at 72,360,729 bp
  • A to T, chromosome 5 at 151,584,997 bp
  • A to G, chromosome 6 at 57,583,206 bp
  • A to G, chromosome 6 at 77,636,839 bp
  • A to C, chromosome 6 at 93,708,070 bp
  • T to C, chromosome 7 at 3,639,706 bp
  • A to T, chromosome 7 at 12,849,774 bp
  • A to T, chromosome 7 at 83,614,962 bp
  • G to A, chromosome 7 at 83,652,567 bp
  • A to G, chromosome 7 at 105,713,987 bp
  • G to T, chromosome 7 at 130,546,992 bp
  • G to T, chromosome 7 at 139,120,712 bp
  • T to A, chromosome 8 at 13,033,958 bp
  • T to C, chromosome 8 at 26,125,966 bp
  • T to G, chromosome 8 at 43,652,061 bp
  • T to C, chromosome 8 at 94,903,371 bp
  • T to A, chromosome 8 at 122,790,672 bp
  • A to T, chromosome 9 at 56,489,832 bp
  • A to T, chromosome 9 at 60,615,161 bp
  • G to T, chromosome 9 at 66,967,687 bp
  • T to A, chromosome 9 at 88,688,754 bp
  • C to A, chromosome 10 at 60,777,546 bp
  • G to A, chromosome 10 at 103,028,443 bp
  • T to G, chromosome 10 at 130,422,803 bp
  • C to A, chromosome 11 at 69,446,649 bp
  • T to C, chromosome 11 at 69,776,322 bp
  • T to A, chromosome 13 at 46,801,313 bp
  • A to T, chromosome 13 at 112,952,801 bp
  • A to T, chromosome 16 at 45,555,769 bp
  • T to G, chromosome 16 at 97,475,368 bp
  • G to A, chromosome 17 at 20,268,376 bp
  • A to G, chromosome 18 at 5,108,675 bp
  • A to G, chromosome 18 at 36,974,735 bp
  • T to A, chromosome 18 at 37,727,543 bp
  • T to C, chromosome 19 at 4,613,909 bp
  • T to C, chromosome 19 at 12,935,584 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6115 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044264-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.