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Strain Name:
C57BL/6J-MtgxR6132Btlr/Mmmh
Stock Number:
044279-MU
Citation ID:
RRID:MMRRC_044279-MU
Other Names:
R6132 (G1)
Major Collection:

Strain Information

Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52463
Homologene: 12735
Kdm5a
Name: lysine demethylase 5A
Synonyms: RBP2, Rbbp2, Jarid1a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 214899
HGNC: HGNC:9886
Homologene: 3419
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 93,939,966 bp
  • T to C, chromosome 1 at 127,850,476 bp
  • A to G, chromosome 1 at 160,146,071 bp
  • A to T, chromosome 2 at 85,430,146 bp
  • A to G, chromosome 2 at 140,159,779 bp
  • A to T, chromosome 2 at 143,078,600 bp
  • C to G, chromosome 3 at 95,591,834 bp
  • C to T, chromosome 4 at 41,517,160 bp
  • G to A, chromosome 4 at 148,156,108 bp
  • C to T, chromosome 4 at 154,895,018 bp
  • T to G, chromosome 5 at 13,523,175 bp
  • T to A, chromosome 5 at 32,910,467 bp
  • T to C, chromosome 5 at 53,200,189 bp
  • G to A, chromosome 6 at 31,431,220 bp
  • A to G, chromosome 6 at 33,758,098 bp
  • C to T, chromosome 6 at 120,374,931 bp
  • G to A, chromosome 7 at 18,957,869 bp
  • T to C, chromosome 7 at 120,361,420 bp
  • A to G, chromosome 7 at 141,889,597 bp
  • T to A, chromosome 8 at 126,434,809 bp
  • ATTGCTGTTT to ATTGCTGTTTGCTGTTT, chromosome 9 at 38,026,540 bp
  • A to T, chromosome 9 at 110,085,779 bp
  • C to T, chromosome 9 at 119,613,695 bp
  • T to A, chromosome 10 at 51,615,260 bp
  • A to C, chromosome 10 at 62,813,300 bp
  • T to C, chromosome 10 at 79,976,440 bp
  • G to A, chromosome 10 at 81,499,196 bp
  • A to T, chromosome 11 at 21,999,406 bp
  • A to G, chromosome 11 at 43,705,628 bp
  • G to T, chromosome 11 at 60,537,995 bp
  • A to G, chromosome 11 at 83,959,457 bp
  • A to G, chromosome 11 at 96,075,024 bp
  • T to A, chromosome 12 at 44,570,224 bp
  • T to C, chromosome 12 at 75,945,147 bp
  • A to C, chromosome 13 at 49,590,367 bp
  • A to G, chromosome 13 at 55,362,225 bp
  • A to C, chromosome 13 at 61,161,768 bp
  • G to T, chromosome 13 at 67,293,296 bp
  • A to G, chromosome 13 at 74,660,282 bp
  • G to T, chromosome 13 at 81,506,076 bp
  • T to A, chromosome 14 at 26,717,911 bp
  • A to G, chromosome 15 at 84,032,972 bp
  • A to T, chromosome 16 at 31,836,241 bp
  • A to G, chromosome 16 at 59,269,004 bp
  • T to C, chromosome 17 at 20,268,404 bp
  • G to T, chromosome 17 at 25,241,861 bp
  • C to T, chromosome 17 at 26,763,403 bp
  • G to T, chromosome 18 at 65,726,355 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • T to A, chromosome 18 at 89,115,646 bp
  • A to G, chromosome 19 at 39,703,414 bp
  • T to A, chromosome 19 at 44,960,861 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6132 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044279-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.