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Strain Name:
C57BL/6J-MtgxR6155Btlr/Mmmh
Stock Number:
044302-MU
Citation ID:
RRID:MMRRC_044302-MU
Other Names:
R6155 (G1)
Major Collection:

Strain Information

Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354
Gabra6
Name: gamma-aminobutyric acid type A receptor subunit alpha 6
Synonyms: Gabra-6, alpha6, GABA-ARalpha6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14399
HGNC: HGNC:4080
Homologene: 20220
Ptch2
Name: patched 2
Synonyms: ptc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19207
HGNC: HGNC:9586
Homologene: 37842
Rasgrp2
Name: RAS, guanyl releasing protein 2
Synonyms: CalDAG-GEFI, Caldaggef1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19395
HGNC: HGNC:9879
Homologene: 4250
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Lgals9
Name: lectin, galactose binding, soluble 9
Synonyms: LGALS35, galectin-9, gal-9, Lgals5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16859
Homologene: 134662
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 17,753,017 bp
  • G to A, chromosome 1 at 39,287,476 bp
  • T to A, chromosome 1 at 43,956,489 bp
  • G to T, chromosome 1 at 130,910,740 bp
  • A to G, chromosome 1 at 133,393,267 bp
  • A to G, chromosome 1 at 167,466,727 bp
  • A to G, chromosome 1 at 178,321,797 bp
  • A to C, chromosome 2 at 5,910,359 bp
  • A to G, chromosome 2 at 89,296,421 bp
  • A to T, chromosome 2 at 101,576,010 bp
  • T to C, chromosome 2 at 125,581,700 bp
  • T to C, chromosome 2 at 155,407,448 bp
  • A to G, chromosome 2 at 179,913,524 bp
  • T to A, chromosome 3 at 87,759,568 bp
  • T to G, chromosome 3 at 92,789,072 bp
  • A to T, chromosome 3 at 142,853,693 bp
  • A to G, chromosome 3 at 144,819,357 bp
  • A to G, chromosome 3 at 152,427,682 bp
  • T to A, chromosome 4 at 117,096,908 bp
  • T to A, chromosome 4 at 143,965,142 bp
  • T to C, chromosome 4 at 152,022,038 bp
  • A to G, chromosome 4 at 155,890,548 bp
  • A to T, chromosome 5 at 33,431,051 bp
  • A to G, chromosome 5 at 35,935,609 bp
  • A to G, chromosome 5 at 72,700,726 bp
  • T to C, chromosome 5 at 104,963,644 bp
  • G to A, chromosome 5 at 124,770,599 bp
  • A to G, chromosome 5 at 124,785,175 bp
  • A to T, chromosome 6 at 88,479,125 bp
  • A to C, chromosome 6 at 128,980,273 bp
  • A to C, chromosome 7 at 28,896,141 bp
  • T to C, chromosome 7 at 41,428,690 bp
  • T to C, chromosome 7 at 108,081,286 bp
  • T to C, chromosome 8 at 15,903,231 bp
  • T to A, chromosome 8 at 45,252,082 bp
  • C to A, chromosome 9 at 54,886,527 bp
  • T to A, chromosome 9 at 66,433,423 bp
  • C to A, chromosome 9 at 110,387,781 bp
  • A to T, chromosome 10 at 20,118,441 bp
  • A to G, chromosome 11 at 5,687,442 bp
  • A to C, chromosome 11 at 30,137,403 bp
  • C to G, chromosome 11 at 34,294,123 bp
  • T to A, chromosome 11 at 42,316,523 bp
  • T to A, chromosome 11 at 49,552,584 bp
  • C to T, chromosome 11 at 78,963,505 bp
  • T to A, chromosome 11 at 97,712,452 bp
  • A to C, chromosome 12 at 40,036,520 bp
  • T to C, chromosome 12 at 57,737,616 bp
  • T to C, chromosome 12 at 116,438,011 bp
  • A to T, chromosome 13 at 38,944,596 bp
  • G to A, chromosome 14 at 29,978,589 bp
  • T to C, chromosome 14 at 50,736,619 bp
  • A to T, chromosome 14 at 70,508,007 bp
  • T to C, chromosome 15 at 6,793,977 bp
  • T to C, chromosome 16 at 16,891,509 bp
  • A to T, chromosome 16 at 18,151,394 bp
  • A to G, chromosome 16 at 93,862,425 bp
  • T to C, chromosome 17 at 19,065,881 bp
  • G to A, chromosome 17 at 36,167,507 bp
  • T to C, chromosome 17 at 71,108,695 bp
  • T to C, chromosome 17 at 83,588,378 bp
  • A to T, chromosome 18 at 32,939,447 bp
  • T to C, chromosome 18 at 37,686,493 bp
  • C to T, chromosome 19 at 4,986,870 bp
  • C to A, chromosome 19 at 6,402,501 bp
  • G to A, chromosome 19 at 6,966,165 bp
  • T to C, chromosome 19 at 48,398,697 bp
  • T to C, chromosome 19 at 56,722,904 bp
  • T to C, chromosome 19 at 58,730,133 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6155 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044302-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.